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Journal of Natural Remedies

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2019

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Rizal, Rizal

Mangosteen Peel Extract (Garcinia mangostana L.) and its Constituents to Lower Lipid Content on Adipogenesis Cells Model (3T3-L1)

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Authors

Wahyu Widowati ¹, Lusiana Darsono ¹, Jo Suherman ¹, Ervi Afifah ², Rizal Rizal ², Yukko Arinta ², Rismawati Laila Qodariah ², Tjandrawati Mozef ³, Tri Suciati ⁴

Affiliations
1 Maranatha Christian University, Bandung - 40164, ID
2 Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung - 40163, ID
3 Research Center for Chemistry, Indonesian Institute of Sciences, Bandung, West Java, ID
4 Bandung Institute of Technology, Bandung, West Java, ID

Source

Journal of Natural Remedies, Vol 18, No 2 (2018), Pagination: 41-48

Abstract

Obesity is one of the risk factor for dyslipidemia incident, cancer, diabetes mellitus, and cardiovascular disease. Treatment of obesity using common commercial drugs shows low rate of success, hence natural products may provide better or more efficient therapeutic approach. Mangosteen (Garcinia mangostana L.) (Clusiaceae) peel extract contains xanthones and it is potentially used as alternative medicine for obesity. This research was done to determine the anti-obesity characteristics of Mangosteen Peel Extract (MPE) and its xanthones α-Mangostin (AM) and γ-Mangostin (GM) on 3T3L1 cell line. Anti-obesity effects of MPE and xanthones were investigated using differentiated-3T3L1 preadipocyte cells. Inhibitory activity of the extract and compounds on the production of Triglyceride (TG), Cholesterol (CHOL), Glucose-6-Phosphate Dehydrogenase (G6PDH) activity, and lipid droplets were examined. MPE and its compound were capable to inhibit the production of TG, CHOL, G6PDH, lipid droplets. MPE 50 μg/mL and GM 75 μM were the most active to lower TG 57.95% and 59.72%, CHOL 33.33% and 31.68%, G6PDH 52.90%, 41.95%, lipid droplets 72.99% and 70.07% respectively. In conclusion mangosteen peel extract and γ-Mangostin are the most active antiobesity compared to α-Mangostin.

Keywords

Glucose-6-Phosphate Dehydrogenase, Mangosteen, Obesity, Xanthones, 3T3-L1 Cells.

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Antioxidant Properties of Red Betel (Piper crocatum) Leaf Extract and Its Compounds

Abstract Views :339 | PDF Views:214

Authors

I. Nyoman Ehrich Lister ¹, Chrismis Novalinda Ginting ¹, Ermi Girsang ¹, Adek Armansyah ¹, Hans Hotma Marpaung ¹, Ade Putra Fratama Sinaga ¹, Rr. Anisa Siwianti Handayani ², Rizal Rizal ²

Affiliations
1 Universitas Prima Indonesia, Jl. Belanga No. 1 simp. Ayahanda, Medan –20118, North Sumatera, ID
2 Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk II no.9 Bandung – 40163, West Java, ID

Source

Journal of Natural Remedies, Vol 19, No 4 (2019), Pagination: 198-205

Abstract

Background: Piper crocatum, also known as red betel, is a potential herbal medicine. Aims: Current study was planned to determine the antioxidant activities of red betel (Piper crocatum Ruiz and Pav.) leaves extract (RBLE) as compared to eugenol and hydroxychavicol compounds. Methods: DPPH radical scavenging, H₂O₂ scavenging, ABTS reduction, and FRAP reduction assay were carried out. Results: In DPPH scavenging, RBLE showed an IC₅₀ value of 3.98 μg/mL, eugenol of 2.98 μg/mL, and hydroxychavicol of 18.00 μg/mL. Meanwhile, H₂O₂ scavenging activity showed an IC₅₀ value of RBLE, eugenol, and hydroxychavicol as 186.33 μg/mL, 97.36 μg/mL, and 41.06 μg/mL respectively. ABTS reduction assay showed an IC₅₀ value of 38.43 μg/mL, 181 μg/mL, and 3.10 μg/mL for RBLE, eugenol, and hydroxychavicol respectively. The highest FRAP reduction activity was shown by Eugenol with a concentration of 50 μg/mL which was equal to 424.67 μM Fe (II)/μg. Conclusion: The RBLE and its compounds (eugenol and hydroxychavicol) have antioxidant activity as indicated by the results of the DPPH scavenging, H₂O₂ scavenging, ABTS reduction, and FRAP reduction assays. However, RBLE had the lowest antioxidant activity compared to other compounds.

Keywords

Eugenol, Free Radicals, Hydroxychavicol, Piper crocatum.

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Rizal, Rizal

Mangosteen Peel Extract (Garcinia mangostana L.) and its Constituents to Lower Lipid Content on Adipogenesis Cells Model (3T3-L1)

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References

Antioxidant Properties of Red Betel (Piper crocatum) Leaf Extract and Its Compounds

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