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Pudi, Rajesh
- New RP-UPLC method for The Simultaneous Determination of Haloperidol and Benzhexol in Tablets
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Authors
Affiliations
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, IN
1 Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2847-2850Abstract
Haloperidol is an anti-psychotic drug and Benzhexol (Trihexyphenidyl) is an anticholinergic drug. A newstability indicating UPLC method has been developed for the simultaneous determination of Haloperidol and Benzhexol and the method was validated. Waters UPLC with Empower software, with Acquity UPLC SB C18 x 1.7μ. column and PDA detector was used for the simultaneous determination of Haloperidol and Benzhexol. A mixture of phosphate buffer and Acetonitrile taken in the ratio 56:44 v/v was used as mobile phase with a flow rate of 0.2 ml/min (UV detection at 245 nm) (Injection volume 1μl). Haloperidol and Benzhexol have shown linearregression equations, y=13361x+476.2(R² = 0.9998) and y =10609 x + 899.5 (R² = 0.9998). The LOD and LOQ are found to be 0.03 μg/mL and 0.10 μg/mL for Haloperidol and 0.03μg/mL and 0.09 μg/mL for Benzhexolrespectively. The present liquid chromatographic method is precise, accurate and can be used for the simultaneous estimation of Haloperidol and Benzhexol tablets.Keywords
Haloperidol, Benzhexol (Trihexyphenidyl), RP-UPLC, Isocratic Mode, Validation.
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