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Unissa, Rahamat
- A Review on Zika Virus
Authors
1 Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad, Telangana, IN
2 Department of Pharmaceutical Chemistry, Acharya and B M Reddy College of Pharmacy, Bangalore, Karnataka, IN
Source
Asian Journal of Pharmaceutical Research, Vol 6, No 3 (2016), Pagination: 183-187Abstract
The Zika virus is a mosquito-transmitted infection common in Africa and Asia, it did not begin spreading widely in the Western Hemisphere, when an outbreak occurred in Brazil. Few people with less immunity, showed rapidly spreading with infection. Millions of people in tropical regions of the Americas now have been infected. The infection causes no symptoms and leads to no lasting harm. Scientific concern is focused on women who become infected while pregnant and those who develop a temporary form of paralysis after exposure to the virus. The World Health Organization has declared the Zika virus an international public health emergency, that it causes birth defects. As many as four million people could be infected by the end of the year.
Officials at the Centers for Disease Control and Prevention have urged pregnant women against travel to more than thirty countries, mostly in the Caribbean and Latin America, where the outbreak is growing. Some pregnant women who have been to these regions should be tested for the infection.
The infection appears to be linked to the development of unusually small heads and brain damage in newborns - called microcephaly - as well as other neurological defects. In adults, the virus is linked to a form of temporary paralysis. The vast majority of Zika infections are transmitted by mosquitoes, sexually transmitted in only few cases (around dozen).
Recently Bharat Biotech, a Hyderabad-based vaccines and bio-therapeutic manufacturer, developed a vaccine to fight against the dreaded mosquito-borne Zika virus.
This review gives an idea about the recent advances or developments available for treating Zika infection.
Keywords
Zika Virus, Aedes aegypti Mosquito, Birth Defects, Microcephaly, Paralysis.
- Purification and Characterization of L-Arginine Deiminase from Vibrio Alginolyticus 1374
Authors
1 Department of Pharmaceutical Biotechnology, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad, Osmania University, Telangana, IN
2 Department of Pharmaceutical Chemistry, Bharat Institute of Technology Pharmacy, Jawaharlal Technological University of Hyderabad, Ibrahimpatnam, Hyderabad, Telangana, IN
Source
Journal of Pharmaceutical Research, Vol 15, No 1 (2016), Pagination: 20-25Abstract
Aim: The aim of our present study was to purify and characterize l-arginine deiminase isolated from marine Vibrio alginolyticus1374.Methodology: Arginine deiminase (AD), an arginine-degrading enzyme, has been used in the treatment of tumours, sensitive to arginine deprivation, such as malignant melanoma and hepatocellular carcinoma. Proteins of microbial origin are always associated with mild to severe hypersensitivities. Moreover, proteins from terrestrial sources were found to exhibit stability problems at physiological environment. Hence considering the fact that the characteristics of the enzyme depends upon sources from which it has been isolated, we have use marine organism for the present study expecting to yield therapeutically and physiologically stable enzyme. ADI production was carried out under optimal conditions by shake flask method. The enzyme thus obtained was purified by ammonium sulphate fractionation, ion exchange and gel permeation chromatography. The purity of the enzyme was further confirmed by sodium dodecyl sulphate polyacrylamide gel electrophoresis, and it was characterized to know its properties.
Results: It was active at physiological pH, showed high substrate specificity towards l-arginine. It also exhibited high salt and temperature tolerance indicating good scope for its industrial and therapeutic applications.
Conclusions: ADI obtained from Vibrio alginolyticus 1374 can be a good candidate for the treatment of human cancers.
Keywords
L-Arginine Deiminase (ADI), Vibrio Alginolyticus 1374, GU726873, Ion Exchange Chromatography, Gel Permeation Chromatography.Full Text
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