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R. Akila ¹, L. Rajendran ², K. Saveetha ², K. Muthumeena ², R. Samiyappan ²

Affiliations
1 Center for Plant Molecular Biology. Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, IN
2 Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, IN

Abstract

Four different organic substrates, viz., decomposed coir pith, dried cow dung, rice bran and tea waste, were tested tor the growth of Trichoderma viride (isolate t). The population (CFU) of T. viride on various substrates was estimated at 20^th, 30^th, 40^th and 60^th day after inoculation by serial dilution plate technique. The population of T. viride was highest on 20^th day in sterilized tea waste (236.30 × 10⁶ cfu), followed by rice bran (152 × 10⁶ cfu), decomposed coir pith (44.35 × 10⁶ cfu), talc (36 × 10⁶ cfu) and dried cow dung (20 × 10⁶ cfu). Sterilized substrate was favourable for the multiplication of T. viride except for dried cow dung. The population increased in all the substrates except talc on 30^th day and declined from 40^th day onwards.

Keywords

Colony Forming Unit (CFU), Organic Substrates, Talc, Trichoderma viride (Isolate 1).

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R. Nadakumar ¹, S. Babu ¹, A. Kandan ¹, T. Raguchander ¹, R. Samiyappan ¹

Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN

Abstract

Eleven Pseudomonas isolates of ten-plant species collected from all over Tamil Nadu were compared for their total cell proteins separated through sodium dodecyl sulphate poly acrylamide gel electrophoresis. AH the isolates showed marked variations among themselves and between fluorescent and non-fluorescent groups. However, similarity was observed with respect to three protein bands with molecular weight of 46, 43 and 32kDa. Data were scored based on the presence or absence of protein bands and cluster analysis was performed. The isolates from same location viz., Coimbatore (PF1, PB2, COP1 and COT1), Sankaran Kovil (PSK1 and PSK2) and host (rice) showed greater level of similarity and occupied same cluster groups.

Keywords

Cell Protein, Electrophoresis, Pseudomonas, SDS-PAGE.

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K. Jayashree ¹, V. Shanmugam ¹, T. Raguchander ¹, A. Ramanathan ¹, R. Samiyappan ¹

Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN

Abstract

Pseudomonas fluorescens strain Pfl, effectively inhibited the mycelial growth of Macrophomina phaseolina, the pathogen causing dry ischolar_main-rot in blackgram and sesame. Application of Pf1 as seed treatment (10g / kg seed) followed by soil application (2.5 kg / ha) against ischolar_main rot effectively supported higher plant growth, better native Rhizobium nodulation and grain yield. Sclerotial number and ischolar_main rot incidence were also greatly reduced. The rhizosphere soil recorded a higher number of Pf I population.

Keywords

Biocontrol, Macrophomina phaseolina, Pseudomonas fluorescens. Root- Rot, Seed Treatment, Soil Application.

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T. Raguchander ¹, K. Jayashree ¹, R. Samiyappan ¹

Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN

Abstract

Among the eight antagonistic microorganisms tested for their efficacy in reducing Fusarium oxyporum f. sp. cubense, Trichoderma viride and Pseudomonas fluorescens were equaly effective under laboratory conditions. The field trails conducted during rabi 1991 and kharif 1992 revealed that dipping of suckers in the suspension of P. fluorescens (10⁶ CFU/ml) or T. viride (10⁶ CFU/ml) along with the application of 500g wheat bran:saw dust inoculum (1:3) of the respective biocontrol agent three months after planting, effectively reduced the Fusarium wilt incidence and produced the highest yield.

Keywords

Biocontrol, Fusarium Wilt of Banana, Fusarium oxysporum f. sp. cubense, Pseudomonas fluorescens, Trichoderma viride.

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