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Ashok Kumar Gupta ¹, K. P. Shivalinge Gowda ², R. N. Umashankar ¹, R. Nandeesh ¹, S. Sreedhar ¹

Affiliations
1 Sree Siddaganga College of Pharmacy, B. H. Road, Tumkur-572 102, Karnataka, IN
2 Dept. of Pharmacology, Sree Siddaganga College of Pharmacy, B. H. Road Tumkur-572102, Karnataka, IN

Abstract

The antioxidant activity is increasing as there is realization of that formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been linked in pathogenesis of several human diseases. Physalis minima L. are commonly known as Country gooseberry, which is widely used in the indigenous system of medicine for the treatment of diuretic, fever and dropsy. The leaves aqueous extract of the plant was studied for its invitro antioxidant activity by two methods viz DPPH radical scavenging assay and Nitric oxide scavenging assay. Its free radical scavenging activity was estimated by IC₅₀ value and the values at various concentrations 5 to 25mg/ml. At 25mg/ml DPPH radical scavenging assay and Nitric oxide assay showed maximum inhibition 92.30% and 75.41% These results clearly indicate that aqueous extract of leaves of Physalis minima is effective in scavenging free radicals and has the potential to be a powerful antioxidant.

Keywords

Physalis minima, DPPH, Antioxidant Activity, Nitric Oxide.

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R. Nandeesh ¹, E. Manjunatha ¹, S. Vijay Kumar ¹

Affiliations
1 Sree Siddaganga College of Pharmacy, B. H. Road, Tumkur - 572 102, Karnataka, IN

Abstract

The present study was designed to evaluate the antioxidant and antimicrobial activity of various extracts of Buxus wallichiana Baill wood. Extraction of wood was done by hot continuous percolation process by using suitable solvents and the concentrated extract was used for the study. Anti oxidant Activity is done by in vitro DPPH (2,2-diphenyl-1-picryl hydrazyl) method and Nitric oxide radical inhibition activity method, where Antibacterial Activity is done by Cup Plate Method. The MEBW (Methanolic extract of Buxus wallichiana) showed an IC₅₀ value of 29.2±2.10, where as ABBW (Aqueous extract of Buxus wallichiana), CEBW(cholroform extract of Buxus wallichiana) and PEBW (Petroleum ether extract of Buxus wallichiana) produced IC₅₀ values of 190±5.33, 647.5±16.43 and 747.5±10.2 respectively in DPPH method. When assayed for Antibacterial Activity MEBW at 500 μg/ml produced zone of inhibition of 26mm against Klebsiella aerogenes organism, against proteus vulgaris organism, CEBW, AEBW at 500 μg/ml produced zone of inhibition of 19 mm. Against bacillus premiles, b. aerogenes and b.coacoagulans MEBW at 500 μg/ml shown zone of inhibition of 17, 19 and 24 mm respectively. The result of this study reveal that Buxus wallichiana Baill could be considered as potential source of natural anti oxidant and anti bacterial plant.

Keywords

Anti Oxidant, Antimicrobial, In vitro, DPPH, Antibacterial.

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