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- Riaz Mahmood
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Journals
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Asokan, R.
- Homology Modeling Deduced Tridimensional Structure of Novel Coleopteran Bacillus Thuringiensis Cry1ib8 Toxin and in Silicon Analysis
Abstract Views :547 |
PDF Views:0
Authors
Affiliations
1 Bio-Pesticide Laboratory, Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessarghatta Lake Post, Bangalore 560 089, IN
2 Bio-Pesticide laboratory (BPL), Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessarghatta lake post, Bangalore 560 089, IN
3 2Post-Graduate Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Jnanasahayadri, Shankaraghatta, Shimoga 577451, Karnataka, IN
1 Bio-Pesticide Laboratory, Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessarghatta Lake Post, Bangalore 560 089, IN
2 Bio-Pesticide laboratory (BPL), Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessarghatta lake post, Bangalore 560 089, IN
3 2Post-Graduate Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Jnanasahayadri, Shankaraghatta, Shimoga 577451, Karnataka, IN
Source
Indian Journal of Biotechnology & Biochemistry, Vol 1, No 1 (2013), Pagination: 37-56Abstract
Cry1I toxins are particularly interesting from an agricultural perspective because of their wide host range. We presented 3D structural model of the novel Coleopteran active Cry1Ib8 δ-endotoxin obtained from native Bt strain using homology modeling. Cry1Ib8 share a common structure contains three flexible domains that participate in the formation of a pore and determine the receptor binding specificity. The pore-forming domain I is composed of residues 60-282. It consists of 10α-helices and two small β-strands. The identified helices and strands are as follows: α1 (E33-K35); α2a (N39-S46); α2b (S56-I60); α3 (Q61-T73); α4 (F78-L93); α5 (K98- V146); α6 (T152-F177); α7a (L185-W210); α7b (A214-L246); α8a (T280-V282); β0 (Q10-L12) and β1a (E182-P184). Domain II comprised of residues 287-487, two helix (α9 F322-A328; α10 P333- L339) and 10 β-strands (β2 T292-T297; β3 V342-S346; β4 M359- P369; β5- L374-N375; β6 T390-Q393; β7 V398-W404; β9 E453- N454; β10 S470-I479 and β11 A486-H493). Domain III is comprised of residues 507-644, has a three antiparallel-sheet sandwich structure present at downstream sites (α11a K655-F664; α13 E678-S690; α14 L697-R718 and α12a is absent) and shows highly conserved β residues. Understanding the mode of action of coleopteran-specific B. thuringiensis toxins through 3-D homology models will aid in the development of novel B. thuringiensis biopesticide with increased efficacy as well as in the development of resistance detection and management strategies.Keywords
3D Structure, Domains, Homology Modeling, Native Bt Strains
References
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- Experimental Investigation of Failure Mechanisms in GFRP Lap Joint Using Acoustic Emission
Abstract Views :187 |
PDF Views:2
Authors
Affiliations
1 Aeronautical Engineering Department, Hindustan University, Chennai, IN
2 Aeronautical Engineering Department, Madras Institute of Technology, Anna University, Chennai, IN
1 Aeronautical Engineering Department, Hindustan University, Chennai, IN
2 Aeronautical Engineering Department, Madras Institute of Technology, Anna University, Chennai, IN
Source
International Journal of Vehicle Structures and Systems, Vol 4, No 2 (2012), Pagination: 48-51Abstract
In this paper, Acoustic Emission (AE) based damage mode identification of Glass Fibre Reinforced Plastic (GFRP) lap joints is detailed using laboratory scale experiments. Bonded single lap joint specimens are fabricated from GFRP laminates. These specimens are subjected to tensile test with on-line AE monitoring. The study of failure mechanisms is facilitated by the choice of different oriented lap joint specimens in which one or two such mechanisms predominate. Range of peak frequencies in each orientation is investigated using frequency analysis. Fast Fourier Transforms enabled calculating the frequency content of each damage mechanism. Parametric plots are used to discriminate the different stages of occurrence of failure mechanism in lap joints. Results have shown that predominate of failure modes in each orientation is used as a key in the study of discrimination of failure modes from AE data in lap joints.Keywords
Composites, GFRP, Lap Joint, Acoustic Emission, Failure Modes.- Performance Analysis of Quality of Service Enabled Temporally Ordered Routing Algorithm Using Ant Colony Optimization in Mobile Ad Hoc Networks
Abstract Views :188 |
PDF Views:2
Authors
R. Asokan
1,
A. M. Natarajan
2
Affiliations
1 Electronics and Communication Engineering Department, Kongu Engineering College, Perundurai, Erode, Tamilnadu, IN
2 Bannari Amman Institute of Technology, Sathiyamangalam, Tamilnadu, IN
1 Electronics and Communication Engineering Department, Kongu Engineering College, Perundurai, Erode, Tamilnadu, IN
2 Bannari Amman Institute of Technology, Sathiyamangalam, Tamilnadu, IN
Source
Journal of Advances in Engineering Sciences, Vol 2, No 1 (2009), Pagination: 11-18Abstract
Mobile Ad hoc NETworks (MANETs) are collection of mobile hosts dynamically forming a temporary network without the aid of any existing infrastructure or centralized control. Quality of Service (QoS) support for MANET is a challenging task due to the dynamic topology and limited resources. Routing in MANET depends on intermediate nodes. The existing QoS based routing solutions for MANET involves with single metric or two metrics. It is important that MANETs should provide QoS support routing such as acceptable delay, jitter and energy in case of multimedia and real time applications. The metrics selection can be from additive or multiplicative or concave or combination of the above. This paper proposes a QoS enabled Temporally Ordered Routing Algorithm (TORA) protocol using Ant Colony Optimization (ACO) called AntTORA. ACO technique is used in this protocol to optimize multiple QoS routing metrics like delay, jitter and energy. Ant-like agents are used in this algorithm to discover and maintain paths with the specified QoS requirements. The performance of TORA and AntTORA are analyzed using network simulator-2. AntTORA produces better performance than TORA in the terms of end-to-end delay, energy, jitter and throughput.Keywords
Mobile Ad Hoc Network, Routing, QoS, TORA and AntTORA.- Cytochrome P450 Isoforms Transcriptional, Larval Growth and Development Responses to Host Allelochemicals in the Generalist Herbivore, Helicoverpa armigera (Hubner) (Lepidoptera:Noctuidae)
Abstract Views :230 |
PDF Views:89
Authors
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru 560 089, IN
2 Department of Biotechnology, St Martin’s Engineering College, Dulapally, Secunderabad 500 014, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake (PO), Bengaluru 560 089, IN
2 Department of Biotechnology, St Martin’s Engineering College, Dulapally, Secunderabad 500 014, IN
Source
Current Science, Vol 111, No 5 (2016), Pagination: 901-906Abstract
Helicoverpa armigera (Hubner) is a polyphagous pest causing severe yield loss in many important crops. Host plants produce allelochemicals to deter insect pests and in response, insects deploy cytochrome P450 monooxygenases (P450s) to detoxify allelochemicals. Understanding the response of P450s to allelochemical exposure is key to effective pest management. We studied the response of seven H. armigera P450 isoforms to different concentrations of three allelochemi-cals (gossypol, tomatine and xanthotoxin) and their effects on insect growth and survival. Allelochemicals strongly induced overexpression of some P450s. CYP6AE14 exhibited the highest overexpression in gossypol treatment. CYP6AE14 and CYP6B7 exhibited higher overexpression in xanthotoxin treatment and CYP6B7 showed the highest overexpression in tomatine treatment. Overall, CYP6AE14 and CYP6B7 were induced by all three allelochemicals. Higher (0.5 and 1.0 μg) concentrations of allelochemicals caused significant larval growth retardation. Interestingly, gossypol showed a hormetic effect, i.e. larval weight was approximately 10% higher at lower (0.025 μg) concentration. Highest larval mortality (53%) was observed in tomatine treatment. These findings would help in identifying suitable P450 isoforms in the management of H. armigera.Keywords
Allelochemicals, Cytochrome P450 Isoforms, Helicoverpa armigera, Pest Management, Real-Time PCR.Full Text
- Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota
Abstract Views :212 |
PDF Views:113
Authors
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Lalitha Anand
1,
Vageeshbabu S. Hanur
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore - 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 2, No 2 (2007), Pagination: 94-98Abstract
Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.Keywords
Growth Regulators, Explant, Brinjal, Transformation.Full Text
- Effect of Antibiotics and Gelling Agents in Transformation of Brinjal (Solanum melongena L.) cv. Manjarigota
Abstract Views :233 |
PDF Views:118
Authors
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Lalitha Anand
1,
Vageeshbabu S. Hanur
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore – 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 2, No 1 (2007), Pagination: 19-25Abstract
A study was conducted to find out the effect of antibiotics and gelling agents on Agrobacterium-mediated transformation using hypocotyl explants of brinjal cv. Manjarigota. Hypocotyl explants of brinjal were found to be sensitive even to the lowest level of kanamycin (25 mg/l) tested. Explants that showed increased callus initiation and regeneration response upon cocultivation with Agrobacterium and on kanamycin at 100 mg/l were selected as this indicated a highly effective selection pressure. Cefotaxime did not affect regeneration response and at 500 mg/l, it effectively inhibited Agrobacterium overgrowth completely on Agrobacterium cocultivated hypocotyl explants. There were marked differences in regeneration response in hypocotyl explants cultured on medium solidified with various gelling agents indicating the influence of gelling agent on the activity of kanamycin in culture medium, which indirectly affects selection and recovery of transformants. Antibiotics and gelling agents could therefore affect, directly or indirectly, transformation of brinjal cv. Manjarigota.Keywords
Solanum melongena, Kanamycin, Cefotaxime, Gelling Agents, Transformation.Full Text
- Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
Abstract Views :222 |
PDF Views:116
Authors
Vageeshbabu S. Hanur
1,
D. P. Prakash
1,
B. S. Deepali
1,
R. Asokan
1,
Y. L. Ramachandra
2,
Riaz Mahmood
2,
Lalitha Anand
1
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore 560 089, IN
2 Department of Biotechnology, Kuvempu University, Shankaraghatta, Shimoga, IN
Source
Journal of Horticultural Sciences, Vol 1, No 2 (2006), Pagination: 116-119Abstract
Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.Keywords
Solanum melongena, Transformation, Hypocotyl, BAP, Preconditioning.Full Text
- Comparative Toxicity of Two Isolates of Bacillus thuringiensis Berliner from Plutella xylostella L. and Papilio demoleus L. to some Important Lepidopteran Pests of Horticultural Crops
Abstract Views :183 |
PDF Views:100
Authors
R. Asokan
1,
Puttaswamy
2
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake (PO), Bangalore – 560 089, IN
2 Department of Entomology, University of Agricultural Sciences, G. K. V. K., Bangalore – 560 065, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake (PO), Bangalore – 560 089, IN
2 Department of Entomology, University of Agricultural Sciences, G. K. V. K., Bangalore – 560 065, IN
Source
Journal of Horticultural Sciences, Vol 2, No 1 (2007), Pagination: 71-72Abstract
Toxicity of two isolates of Bacillus thuringiensis viz. KPx-1 and IPd-1 isolated from diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae), and citrus butterfly, Papilio demoleus (Lepidoptera: Papilionidae), were tested against cabbage leaf webber, Crocidolomia binotalis, hairy caterpillar, Diacrisia obliqua and tomato fruit borer, Helicoverpa armigera. Among these, C. binotalis was highly susceptible (28.4 and 26.0 ng/cm2, for KPx-1 and IPd-1, respectively), while, H. armigera was the least susceptible (9.5 and 10.0 μg/ml, for KPx-1 and IPd-1, respectively).Keywords
Bacillus thuringiensis, Isolates, Toxicity.Full Text
- Role Of Transportation In Tourism Industry In Sikkim State, India
Abstract Views :131 |
PDF Views:2
Authors
Source
International Journal of Innovative Research and Development, Vol 2, No 6 (2013), Pagination:Abstract
The purpose of this paper is to examine the role of transportation in development of tourism industry in Sikkim state, India. Further, this study analyses the tourist inflow and the means of tourism transport in Sikkim state. This study is based on secondary data. Data have been taken from the Tourism Department and Transport department, Government of Sikkim. Simple percentage and multi-average growth rate have been used to have a comparative study of tourists’ inflows and increasing taxis in Sikkim. Further, Pearson correlation has been used to test the hypothesis. It is found that the taxis are used as a means of tourism transport in Sikkim. The number of registered tourists’ transport is increasing every year in Sikkim along with the inflows of tourists in the state but not in same rate. It was observed that an average annual growth percentage rate of tourists’ inflow is 12.76 percent and for taxis is 4.67 percent. The paper is limited to the study of road-transport and tourism in Sikkim. Since, taxis are the main mode of tourist transport, only the total number of taxis and its average annual growth rate is compared with the average growth rate of tourists in Sikkim. The percenatge rate of increase in taxis should increase equally to percentage increase in tourists inflows to meet the demand of tourism transport in the future. To meet the increasing demand of tourism transport, Government and stakeholders should give more focus on the improvement of tourism transport in state.
Keywords
Transportation, tourism transport, road transport, taxis, SikkimFull Text
- Effect of Intra-Ply Hybridization of Carbon-Aramid/Epoxy Laminates under Tension-Tension Fatigue Loading
Abstract Views :228 |
PDF Views:111
Authors
Affiliations
1 School of Aeronautical Sci., Hindustan Institute of Tech. and Sci., Chennai, IN
1 School of Aeronautical Sci., Hindustan Institute of Tech. and Sci., Chennai, IN
Source
International Journal of Vehicle Structures and Systems, Vol 10, No 6 (2018), Pagination: 384-388Abstract
The objective of the research is to investigate the fatigue life of intra-ply hybrid Carbon-Aramid laminate with Epoxy resin in on-axis and off-axis directions. Three different off-axis angles of 15°, 30° and 45° were considered for the present work. The intra-ply hybridization is used to combine the superior mechanical properties of Carbon fibre with excellent elongation-to-failure property of Aramid fibre in the same lamina. The fatigue test was performed using load control using a frequency of 5Hz. The fatigue behaviour was studied for Carbon/Epoxy, Aramid/Epoxy, Carbon-Aramid/Epoxy, Carbon-Aramid/Epoxy - 15°, Carbon-Aramid/Epoxy - 30° and Carbon-Aramid/Epoxy - 45° with the stress ratio of R = 0.1. The ultimate tensile strength decreases progressively for Carbon/Epoxy, Carbon-Aramid/Epoxy, Aramid/Epoxy, Carbon-Aramid/Epoxy - 15°, Carbon-Aramid/Epoxy - 30° and Carbon-Aramid/Epoxy - 45°. The effect of off-axis loading indicates that the increase of fibre angle influences the decrease in tensile strength and fatigue life.Keywords
Lamina, Carbon-Aramid Reinforced Epoxy, Interlaced, Fatigue Life, Intra-Ply.Full Text
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