Informatics Publishing Limited

H. M. Ranganatha ¹, Shreekanth S. Patil ², S. M. Manjula ², B. N. Arvind Kumar ³, P. Swathi ¹

Affiliations
1 Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Dharwad, Karnataka, IN
2 Agricultural Research Station, (U.A.S.) Hebballi Farm, Dharwad, Karnataka, IN
3 Department of Agronomy, College of Agriculture, University of Agricultural Sciences, Dharwad, Karnataka, IN

Abstract

An investigation was carried out to assess the combining ability and nature of gene action in respect of seed cotton yield and its component traits in 54 new hybrids developed by crossing 9 lines with 6 testers of upland cotton in line × tester mating design in Randomized Block Design with two replications during Kharif 2010 at Main Agricultural Research Station, University of Agricultural Sciences, Dharwad. The variance among the lines (2.5 % span length and micronaire), testers and line × tester interaction (2.5 % span length, fibre strength and micronaire) were highly significant for the characters indicating predominance of non-additive gene action in genetic control of these traits. Magnitude of sca variance was higher than the gca variance for majority of the traits. The ratio of gca/ sca variance indicated preponderance of non-additive type of gene action, which is an integral component of the genetic architecture of different characters in the material used in cotton. L₁ and L₉ among the lines and T₁ and T₂ among the testers were identified as good general combiners indicating their ability in transmitting additive genes in the desirable direction to their progenies. Highly significant sca effects were observed in most of the hybrids for all the characters studied and good specific combiners for different characters involved parents with high × high, high × low, low × high and low × low general combinations.

Keywords

Combining Ability, Upland Cotton, Span Length, Fibre Strength

Full Text

     

P. Swathi ¹, K. Rajeswar Dutt ¹, K. N. V. Rao ¹, M. Alagar Raja ¹

Affiliations
1 Nalanda College of Pharmacy, Charlapally, Nalgonda, Telangana, IN

Abstract

A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validation of Sofosbuvir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Hypersil C₁₈ (4.6×150mm, 5μ) column using a mixture of Methanol (100% v/v) as the mobile phase at a flow rate of 1.0 mL/min, the detection was carried out at 265 nm. The retention time of the Sofosbuvir was 3.515 ±0.02min. The method produce linear responses in the concentration range of 20-100μg/mL of Sofosbuvir. The method precision for the determination of assay was below 2.0% RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Keywords

Sofosbuvir, RP-HPLC, Validation, Tablet Dosage Forms.

Full Text

     

B. Saraswathi ¹, R. Jagadeesh Reddy ¹, P. Swathi ¹, G. Manasa ¹

Affiliations
1 Department of Pharmaceutics, Mother Teresa Pharmacy College, Sathupally, Khammam Dist, 507303, IN

Abstract

The aim of the present study was to develop floating formulation of Procainamide to maintain constant therapeutic levels of the drug for over 10 hrs. Various eudragit polymers were employed as polymers. Procainamide dose was fixed as 20 mg. Total weight of the tablet was considered as 300 mg. Polymers were used in the concentration of 30,60 and 90 mg concentration. All the formulations were passed various physicochemical evaluation parameters and they were found to be within limits. Whereas from the dissolution studies it was evident that the formulation (F2) showed better and desired drug release pattern i.e., 99.4 % in 12 hours. It contains the eudragit polymer Procainamide as sustained release material. It followed zero order release kinetics mechanism.

Keywords

Procainamide, Eudragit (L 100, S 100, RSPO) Floating Tablets.

Full Text