Informatics Publishing Limited

B. B. Sahu , Bikash Sarkar , B. C. Mahapa ¹, N. K. Barik ¹, B. R. Pillai ¹, P. L. Lalrinsanga ¹, G. Patra ¹, P. Jayasankar ¹, M. k. Ghosal ², P. R. Bhatnagar ³

Affiliations
1 Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar Odisha, IN
2 Department of Farm Machinery and Power, College of Agricultural Engineering and Technology, Orissa University of Agriculture and Technology, Bhubaneswar Odisha, IN
3 Central Institute of Post Harvest Engineering and Technology, Ludhiana Punjab

Abstract

A handy low cost device for dispensing the microbound larval food for prawn hatcheries was developed by the AICRP on APA (ICAR), CIFA, Bhubaneswar Centre. The unit was made from low density polyethylene plastic (LDPE) material.The material is having unique food grade properties like has excellent resistance to water, moisture and most organic solvents. The system works with little applied force on the plunger, moves down in the cylinder and resulting dispensing of larval food in predetermined quantity and sizes for prawn and fish hatchery operation. The microbound diet of 300-1000 micron provided the texture and size of the particles for larvae and was observed to be very much acceptable for completing the whole larval cycle.

Keywords

Larval Feed Dispenser, Prawn And Fish Hatcheries, Microbound Diet

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B. B. Sahu ¹, B. R. Pillai ², G. Patra ², P. L. Lalrinsanga ², S. Ferosekhan ², P. Jayasankar ²

Affiliations
1 Central Institute of Freshwater Aquaculture, Kausalyaganga, BHUBANESWAR (ODISHA), IN
2 Central Institute of Freshwater Aquaculture, Kausalyaganga, BHUBANESWAR (ODISHA), IN

Abstract

A high moisture (80-85%), fish gel food (FGF) containing 97 per cent fish gel dispersion, sodium alginate, vitamins, mineral and micronutrient supplement was compared to newly hatched live Artemia nauplii as an exclusive diet for the culture of larval freshwater prawn Macrobrachium rosenbergii. M. rosenbergii larvae were gradually weaned from Artemia nauplii to fish gel food (FGF) at their different stages. The feeding regime (AGIOG) in which larvae were weaned on to FGF resulted in the highest (P<0.05) yield of 22.65 ± 6.87 per cent PL/L. After day 11, the survival of larvae fed Artemia alone (A) regimen decreased and at day 25, the value (67.16 ± 4.62% with a corresponding production rate of 20.15 ± 1.88 PL/l) was significantly lower (P<0.5) than those of larvae fed the diets AG10G and AG 8G. The larvae fed with the weaning diets developed faster at later stages than those fed Artemia alone. FGF is composed of readily available ingredients, the diet contains 73.83 per cent crude protein, and 7.24 per cent ether extract, is easy to prepare, and has good water stability and buoyancy. The characteristics of diet suggest good potential for successful use in larvae culture.

Keywords

Freshwater Prawn, Larval Feed, Weaning, Prawn Zoea, Artemia nauplii.

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S. Patra ¹, G. Mishra ¹, S. K. Dash ², D. K. Verma ¹, S. Nandi ³, P. Jayasankar ³, P. Routray ¹

Affiliations
1 Cryobiology Laboratory, Aquaculture Production and Environment Division, ICAR – Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751 002, IN
2 Berhampur University, Berhampur 760 007, IN
3 Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751 002, IN

Abstract

Cryopreservation of gametes and germ cells is an essential tool for germplasm conservation and improvement of productivity in aquaculture. Here, transplantation worthiness of isolated cryopreserved germ cells (GCs) of Indian major carp, rohu Labeo rohita has been tested by their viability and colonization ability in the allogenic host (Catla catla). GCs were cryopreserved using dimethyl sulphoxide (DMSO), ethylene glycol (EG) and glycerol. Rohu GCs were successfully cryopreserved with significantly higher viability using slow cooling rate of -1C/min and a medium containing 1.4 M DMSO compared to EG and glycerol. It was found that more than 70% GCs were viable following this method. Transplantation experiment revealed that frozen/thawed GCs colonized and proliferated in the gonad of the recipients. Hence, this technique of transplantation of GC into adult gonads paves the way for further applications in surrogate animal development.

Keywords

Carp, Cryopreservation, Toxicity, Transplantation, Viability.

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