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Lalthanpuii, P. B.
- Study on the Phytochemical Constituents and Some Biological Activities of the Toothache Plant Acmella oleracea, Cultivated in Mizoram, India
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Authors
Affiliations
1 Department of Zoology, Pachhunga University College, Aizawl 796001, Mizoram, IN
2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk 796017, Mizoram, IN
1 Department of Zoology, Pachhunga University College, Aizawl 796001, Mizoram, IN
2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk 796017, Mizoram, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 9, No 3 (2017), Pagination: 152-155Abstract
One of the most widely used medicinal plants among the Mizo people of India is Acmella oleracea (L.) R.K. Jansen (family Asteraceae). In addition to its medicinal properties, it is regularly used as vegetable. Its traditional applications include the treatments of anaemia, body ache, cancer, gastric ulcer and infections, gingivitis, gout, inflammations, laceration, malaria, stammering, and intestinal helminthiasis. In this study, different extracts of the whole plants were prepared using hexane, chloroform and methanol. The methanol extract indicated the presence of highest number of bioactive compounds, such as carbohydrates, phytosterols, flavonoids, and tannins. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to determine the antioxidant activity. The plant extract exhibited similar concentration-dependent scavenging activity of the free radicalas that of butylated hydroxytoluene (BHT). The total antioxidant activity was estimated against ascorbic acid, and it showed higher value than BHT.Antibacterial activity was studied using the methanol extract and tetracycline as a standard reference. Corresponding to that of the antibiotic, 10 and 20 mg/ml of the plant extract were treated with two Gram-negative bacteria P. aeruginosa and Escherichia coli, and two Gram-positivebacteria Staphylococcus aureus and Bacillus subtilis. However, the plant extract showed no antibacterial activity on these species. Nonetheless, the study provides important information on the basic pharmacological properties of the plant.Keywords
Acmella oleracea, Antioxidant Activity, Medicinal Plant, Antimicrobial Activity, Phytochemical.
References
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- Anticancer and DNA-Protecting Potentials of Spilanthes acmella (Toothache Plant) Grown in Mizoram, India
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Authors
Affiliations
1 Department of Life Sciences, Pachhunga University College, Aizawl – 796001, Mizoram, IN
1 Department of Life Sciences, Pachhunga University College, Aizawl – 796001, Mizoram, IN
Source
Journal of Natural Remedies, Vol 19, No 2 (2019), Pagination: 57-63Abstract
The toothache plant, Spilanthes acmella (L.) Murray, is an important culinary and medicinal plant. Here, we study its probable properties as a protective agent for DNA damage and cell proliferation, the two molecular events in oncogenesis. Plant extract was prepared by hot extraction using methanol as solvent. Various chemical tests were performed to collectively understand its free radical-scavenging activity, including 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, potassium ferricyanide, hydroxyl radical, and hydrogen peroxide assays. Free radicals used or produced in these assays represent those that cause DNA damages in cells. The plant extract showed free radical-scavenging activity in all the tests and was as effective as standard drugs. Cancer cell lines such as Dalton’s lymphoma ascites (DLA) and Chinese hamster lung carcinoma (V79) cells were used for anticancer assays. The plant extract showed anticancer activity only on lymphoma cells; but there was no discernible inhibition on carcinoma. The study shows that S. acmella is a promising source of therapeutic agent in the prevention of cancer development and DNA damage.Keywords
Chinese Hamster Lung Carcinoma, Dalton’s Lymphoma Ascites, Free Radical Scavenging, Medicinal Plant, MTT Assay.Full Text
References
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- Some Phytochemical, Antimicrobial and Anticancer Tests for an Aqueous extract of Acmella oleracea
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Authors
Affiliations
1 Department of Life Sciences, Pachhunga University College, Aizawl 796001, Mizoram, IN
2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk 796017, Mizoram, IN
3 Department of Zoology, Mizoram University, Tanhril 796004, Mizoram, IN
1 Department of Life Sciences, Pachhunga University College, Aizawl 796001, Mizoram, IN
2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk 796017, Mizoram, IN
3 Department of Zoology, Mizoram University, Tanhril 796004, Mizoram, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 3033-3037Abstract
Acmella oleracea (family Asteraceae) is known for its diverse applications such as food supplement, vegetable, antioxidant, antineoplastic, antimicrobial, ornamental plant and pig fodder. We prepared an aqueous extract from which the presence of alkaloids, tannins and saponins were detected. These chemical groups have been known for their wide-ranging therapeutic properties. The antibacterial activity was tested on Gram-negative bacteria such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, and a Gram-positive species Bacillus subtilis. The plant extract showed no activity compared to that of the standard antibiotic tetracycline. Cytotoxic activity was also studied on cancer cell lines. We found an inverse relationship between treatment concentration and percent inhibition for HeLa (ATCC® CCL-2™) and V79 (ATCC® CCL-93™), however, the plant extract showed negligible effect on Dalton’s lymphoma ascites even at the highest concentration tested. The lower degree of inhibitions observed at higher concentrations for HeLa and V79 suggests that the plant extract might confer proliferative effect rather than antimitogenic effects on the cancer cells. The results indicate that A. oleracea has unique medicinal properties, which in turn provide the rationale for further investigations.Keywords
Acmella oleracea, Medicinal Plant, Antibacterial, Cytotoxicity, Cancer Cells.References
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