Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Kumar, Om
- Preservation Plots and their Survival Status (1927 to 2007)
Abstract Views :256 |
PDF Views:0
Authors
Source
Indian Forester, Vol 135, No 6 (2009), Pagination: 853-857Abstract
No abstract
- Global Environment Security : Role of Tropical Forest Therein
Abstract Views :184 |
PDF Views:0
Authors
Source
Indian Forester, Vol 118, No 5 (1992), Pagination: 367-370Abstract
The paper summarizes the changing pattern of environmental degradation, identifies causes of these catastrophes and discusses the role of tropical forests in ensuring the global environmental security. Steps have been suggested which will assist in protection, preservation, conservation and afforestation of tropical forests. Need to have a partnership of developed countries in preserving the global environment an regreenlng of the earth has been stressed.- Ecological Rehabilitation of Surface Mined Lands
Abstract Views :264 |
PDF Views:0
Authors
Source
Indian Forester, Vol 117, No 6 (1991), Pagination: 485-493Abstract
In this paper various measures for reclamation of surface mined lands have been suggested to keep the ecological balance and sustainable ecosystem.- Biological Diversity in Surface Mined Areas After Reclamation
Abstract Views :236 |
PDF Views:0
Authors
Source
Indian Forester, Vol 115, No 7 (1989), Pagination: 475-482Abstract
Diversity of plants on reclaimed surface mine areas of rock phosphate mines at Maldeota (18 km from Dehra Dun) has been evaluated. Results of the study shows that diversity of plants has increased significantly after 4 years of reclamation and is even higher than the adjoining natural areas as well as lower and upper Himalayan moist temperate forest (sub-group 12 Cl and 12 C2).- Water Quality Status in Doon Valley Forest
Abstract Views :200 |
PDF Views:0
Authors
Source
Indian Forester, Vol 115, No 8 (1989), Pagination: 526-535Abstract
The studies on water quality status have been carried out on the perennial streams. Song, Suswa and Reh, selecting the eight representatives sites. Temperature, conductivity and pH of water samples analysed have been described in this paper.- Ecological Approach towards Reclaiming Mined Ecosystem
Abstract Views :185 |
PDF Views:1
Authors
Source
Indian Forester, Vol 115, No 12 (1989), Pagination: 875-883Abstract
Present paper emphasizes the role of ecological approach in restoring the degraded mined ecosystem. The four important steps for an effective eco-restoration programme are (i) assessment of The problems, (ii) ecological maintainance of essential processes, (iii) preservation of genetic diversity and (iv) sustainable utilization of species and ecosystems. A case study of eco-restoration of Durmala rock phosphate mine has been presented which shows that after four years of reclamation, the area stabilizes and provides bospitable environment for bumans as well as animal species of birds, reptiles, rabbits etc.- Environmental Impact of Distillary Effluents on the forest Recreation Spot at Lacchiwala
Abstract Views :143 |
PDF Views:0
Authors
Source
Indian Forester, Vol 112, No 11 (1986), Pagination: 1021-1025Abstract
No Abstract- Phytosociology and Standing Crop Biomass of Five Different Forest Types of East Dehra Dun Forest Division
Abstract Views :180 |
PDF Views:0
Authors
Source
Indian Forester, Vol 110, No 8 (1984), Pagination: 791-801Abstract
no abstract- Community Structure and Biomass Production in a Northern Tropical Dry Mixed Deciduous Forest of East Dehra Dun Division
Abstract Views :218 |
PDF Views:0
Authors
Source
Indian Forester, Vol 110, No 9 (1984), Pagination: 954-957Abstract
no abstract- A Pilot Project to Develop in Vivo Detection of Ricin through Fluorescence Sandwich ELISA Technique
Abstract Views :670 |
PDF Views:0
Authors
Affiliations
1 Department of Biochemistry Saraswathi Institute of Medical Sciences Hapur, Panchsheel nagar, U.P., IN
2 Department of Biochemistry, University College of Medical Sciences Delhi, IN
3 Division of Pharmacology and Toxicology, Defence Research & Development Establishment, Gwalior, IN
1 Department of Biochemistry Saraswathi Institute of Medical Sciences Hapur, Panchsheel nagar, U.P., IN
2 Department of Biochemistry, University College of Medical Sciences Delhi, IN
3 Division of Pharmacology and Toxicology, Defence Research & Development Establishment, Gwalior, IN
Source
Indian Journal of Public Health Research & Development, Vol 4, No 3 (2013), Pagination: 217-221Abstract
Aims & Objective: In the present study our main objective was to develop a fluorescence sandwich ELISA assay for in vivo detection of ricin from plasma of mice after intoxication. Ricin (also called RCA-II or RCA60), one of the most potent toxins and documented bioweapons, is derived from castor beans of Ricinus communis.
Material & Method: The assay was performed in plasma obtained from ricin treated and control mice after 30 minutes of exposure. The assay utilizes an antiricin rat polyclonal antibody to adsorb ricin from plasma of ricin treated mice. The rabbit polyclonal antiricin antibody is then used to form a sandwich, and anti-rabbit FITC-conjugate allows development of fluorescence. The developed fluorescence was read at an excitation-emission wavelength of 485/528nm. The graph was drawn by plotting the ricin concentration against the fluorescence (rf) values.
Result: We were able to detect more than 19.5ng/ml of ricin in assay buffer, 28ng/ml in diluted plasma (1:20480) at the dose of 10mg/kg and 27ng/ml of diluted Plasma (1:80) at the dose of 5mg/ kg body weight. The coefficient of variation ranged from 22% for ricin in assay buffer, 9% for 5mg/ kg body weight dose plasma, 14% for 10mg/kg body weight dose plasma and 4% for non treated control plasma.
Conclusions: The detection of ricin from serum/plasma of individuals exposed to ricin will be quite difficult at lethal dose, estimated for humans to be 1 to 10μg per kg body weight following inhalation or injection. Hence, more efforts are needed for the detection of ricin at its lethal exposure dose.
Keywords
Ricin, In vivo Detection, Fluorescence Sandwich ELISAReferences
- Greenfield RA, Brown BR, Hutchins JB, Iandolo JJ, Jackson R, et al. Microbiological, biological, and chemical weapons of warfare and terrorism. Am J Med Sci. (2002), 323:326–40.
- Balint GA. Ricin: the toxic protein of castor oil seeds. Toxicology (1974), 2:77–102.
- Franz DR, Jaax NK. Ricin Toxin. Medical Aspects of Chemical and Biological Warfare; Chapter (1997), 32:631–642.
- Doan LG. Ricin: mechanism of toxicity, clinical manifestations, and vaccine development. J Toxicol Clin Toxicol (2004), 42:201–208.
- Audi J, Belson M, Patel M, Schier J, Osterloh J. Ricin poisoning: a comprehensive review. Jama (2005), 294:2342–2351.
- Lin TT, Li SL. Purification and physicochemical properties of ricins and agglutinins from Ricinus communis. Eur J Biochem (1980), 105:453–459.
- Nagler-Anderson C. Man the barrier: Strategic defences in the intestinal mucosa. Nat Rev Immunol (2001), 1:59–67.
- Knigh B. Ricin-A potent homicidal poison. British Medical Journal (1975), 1:350-351.
- Crompton R, Gall D. Georgi Markov-Death in a pellet, Medico-legal J (1980), 48:48-62.
- Shea D, Gottron F. Ricin: Technical Background and Potential Role in Terrorism, Congressional Research Service, The Library of Congress, updated February (2004), accessed on October 28, 2008.
- Lubelli C, Chatgilialoglu A, Bolognesi A, Strocchi P, Colombatti M and Stirpe F. Detection of ricin and other ribosome-inactivating proteins by an immunopolymerase chain reaction assay. Analytical Biochemistry (2006), 355:102-109.
- Pradhan S, Kumar O. Detection of Ricin by Comparative Indirect ELISA Using Fluorescence Probe In Blood And Red Blood Cells. J Med CBR Def (2009), 7:508-518.
- Weil CS. Tables for convenient calculation of median-effective dose, (LD50 or ED50) and instructions in their use. Biometrics (1952), 8:249– 263.
- Endo Y, Tsurugi K. The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA. J Biol Chem. (1988), 263:8735–8739.
- Olsnes S. The history of ricin, abrin and related toxins. Toxicon (2004), 44:361–370.
- Lord JM, Roberts LM, Robertus JD. Ricin: structure, mode of action, and some current applications. Faseb J (1994), 8:201–208.
- Godal A, Olsnes S and Pihl A. Radioimmunoassay of abrin and ricin in blood. J. Toxicol. Environ. Health (1981), 8:409-417.
- Poli MA, Rivera VR, Hewetson JF and Merill GA. Detection of ricin by colorimetric and chemiluminescence ELISA. Toxicon (1994), 32:1371-1377.
- Shyu RH, Chiao DJ, Liu HW and Tang SS. Monoclonal antibody based enzyme immunoassay for detection of ricin. Hybrid Hybridol (2002a), 21:96-73.
- Shyu RH, Shyu HF, Liu HW and Tang SS. Colloidal gold based immunochromatographic assay for detection of ricin. Toxicon (2002b), 40:255-258.
- Rubina AY, Dyukova VI, Dementieva EI, Stomakhin AA, Nesmeyanov VA, Grishin EV and Zasedatelev AS. Quantitative immunoassay of biotoxins on hydrogel based protein microchips. Anal. Biochem (2005), 340:317-329.
- Cook DL, Jonathan D and Griffiths GD. Retrospective identification of ricin in animal tissues following administration by pulmonary and oral routes. Toxicology (2006), 223:61-70.
- Malcolm A and Brian P. A sensitive immunosorbent assay for the detection of ricin in blood, Defence Science and Technology Organisation (DSTO), (1997), Report No. DSTOTR- 0572.