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Sharma, Nirmal
- In Vitro Regeneration of Grape (Vitis vinifera L.) Cv. Perlette
Abstract Views :403 |
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Authors
Mahital
1,
Barinder Singh
2,
Nirmal Sharma
3,
Rajesh Kumar
3,
Arti Sharma
3,
R. M. Sharma
3,
V. K. Wali
3,
A. M. Parmar
3
Affiliations
1 Division of Fruit Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, J&K, IN
2 Division of Fruit Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, J&K, IN
3 Division of Fruit Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, J&K, IN
1 Division of Fruit Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, J&K, IN
2 Division of Fruit Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, J&K, IN
3 Division of Fruit Science, Sher-e- Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu, J&K, IN
Source
Asian Journal of Bio Science, Vol 8, No 1 (2013), Pagination: 19-24Abstract
Factors like slow and seasonal multiplication and infection with pathogens have constrained the use of conventional plant propagation methods, thus lead to development of new and novel methods of propagation like in vitro multiplication which ensures the production of virus and disease free elite planting material in large numbers. In the present work, in vitro regeneration protocol has been standardized for grape cv. Perlette which is conventionally propagated through hardwood cuttings. The highest explant establishment (86.66%) and lowest days (12.00) for explants establishment were obtained in MS medium supplemented with 1.0 mg/l BAP and 1.0 mg/l kinetin. Best shoot proliferation (3.33 shoots/ culture) was obtained in MS medium fortified with 1.0 mg/l BAP and 0.5 mg/l kinetin. The media constituting MS + 1.0 mg/l NAA was best for ischolar_maining of in vitro raised shoots, yielding 73.33 per cent ischolar_maining with an average ischolar_main length of 4.43 cm. The most suitable potting media for in vitro raised plantlet hardening of grape cv. Perlette constituted sand (1part) soil (1part) FYM (1part) vermiculture (1part) which resulted in 73.33 per cent plantlet survival.Keywords
Grape, in Vitro Regeneration, Tissue Culture, Vitis vinifera L., PerletteReferences
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- Multivariate Analysis and Choice of Parent for Hybridization in Apple (Malus X domestica Borkh.)
Abstract Views :270 |
PDF Views:0
Authors
Affiliations
1 Department of Fruit Breeding and Genetic Resourses, Dr. Y. S. Parmar University of Horticulture and forestry, Nauni, Solan, H.P., IN
2 Division of Fruit Science, Sher-e-kashmir University of Agricultural Sciences and Technology, Jammu, J&K, IN
1 Department of Fruit Breeding and Genetic Resourses, Dr. Y. S. Parmar University of Horticulture and forestry, Nauni, Solan, H.P., IN
2 Division of Fruit Science, Sher-e-kashmir University of Agricultural Sciences and Technology, Jammu, J&K, IN
Source
Asian Journal of Bio Science, Vol 8, No 1 (2013), Pagination: 39-42Abstract
Medium quantum of genetic divergence was observed among sixteen apple genotypes under the present study. All the genotypes, on the basis of total variability were grouped into four distinct clusters. Maximum number of cultivars were accommodated in cluster IV (Fuji, Gala, Jonadel, Jonagold, Red Fuji, Royal Gala and Spijon) followed by cluster I (Arlet, Ruspippin, Sinta and Summerred), Cluster III (Crimson Gold, Elstar and Neomi) and cluster II ('Spartan' and 'Quinte'). Cluster IV had highest intra cluster value (9.32) so was most divergent and cluster I having least intra cluster value (8.20) was least divergent. Highest value (30.331) for inter cluster distance was recorded between cluster I and II while it was lowest (9.994) between cluster III and IV. Cluster means were maximum in cluster II followed by cluster I, cluster III and cluster IV. Neomi was best cultivars for fruit yield/plant, fruit length, fruit diameter, fruit weight, total sugars and non-reducing sugars, however, Jonagold was best for TSS. Cultivars Spartan, Elstar, Royal Gala, Jonagold and Summerred would prove best for different vegetative characters.Keywords
Apple, Cluster analysis, D2 statistics, Genetic divergenceReferences
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