Refine your search
Collections
Co-Authors
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Joshi, Neha
- A Method for Small Scale Genomic DNA Isolation from Chickpea (Cicer arietinum L.) Suitable for Molecular Marker Analysis
Abstract Views :542 |
PDF Views:121
Authors
Affiliations
1 B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, IN
1 B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, IN
Source
Indian Journal of Science and Technology, Vol 3, No 12 (2010), Pagination: 1214-1217Abstract
We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure&intact DNA.Keywords
DNA Isolation, Cicer arietinum, PCR AnalysisReferences
- Do N and Adams RP (1991) A simple technique of removing plant polysaccharides contaminants from DNA. Biotechniques. 10, 162-166.
- Doyle JJ and Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19, 11-15.
- Doyle JJ and Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus. 12, 13-15.
- Fang G, Hammer S and Groumet R (1992) A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biofeedback. 13, 52-54.
- Lodhi MA, Ye GN and Weeden NF (1994) A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol. Biol. Rep. 12, 6-13.
- Loomis MD (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 31, 528–544.
- Mace ES, Buhariwalla HK and Crouch JH (2003) A high-throughput DNA extraction protocol for tropical molecular breeding programs. Plant Mol. Biol. Rep. 21, 459a-459h.
- Merlo DJ and Kemp JD (1976) Effect of polysaccharides on kinetics of DNA. Plant Physiol. 58, 1522-1526.
- Pandey RN, Adams RP and Flournoy LE (1996) Inhibitions of random amplified poly-morphic DNAs (RAPDs) by plant polysaccharides. Plant Mol. Biol. Rep. 14, 17-22.
- Paterson AH, Brubaker CL and Wendel JF (1993) A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol. Biol. Rep. 11, 122-127.
- Pikart MJ and Villeponteau B (1993) Suppression of PCR amplification by high levels of RNA. Biotech.14, 24-25.
- Porebski S, Bailey LG and Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep. 15, 8–15.
- Sambrook J, Fritch EF and Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Springer Harbor Laboratory Press, Cold Spring Harbor, NY.
- Scott KD and Playford J (1996) DNA extraction technique for PCR in rain forest plant species. Biotech. 20, 974-978.
- Sharma AD, Gill PK and Singh P (2002) DNA isolation from dry and fresh samples of polysaccharide rich plants. Plant Mol. Biol. Rep. 20, 415a– 415f.
- Sharma KK, Lavanya M and Anjaiah V (2000) A method for isolation and purification of peanut genomic DNA suitable for analytical applications. Plant Mol. Biol. Rep. 18, 393a-393h.
- Varshney RK, Horres R, Molina C, Nayak S, Jungmann R, Swamy P, Winter P, Jayashree B, Kahl G and Hoisington DA (2007) Extending the repertoire of microsatellite markers for genetic linkage mapping and germplasm screening in chickpea. J. SAT Agri. Res. 5(1), 1-3.
- Weishing K, Nybom H, Wolff K and Meyer W (1995) DNA isolation and purification. In: DNA fingerprinting in plants and fungi, CRC Press, Boca Raton, Florida, pp: 44-59.
- Organizational Work-Life Balance Policies:A Study on Indian Insurance and Banking Sector
Abstract Views :382 |
PDF Views:102
Authors
Affiliations
1 Institute of Professional Excellence & Management, Ghaziabad, U.P., IN
1 Institute of Professional Excellence & Management, Ghaziabad, U.P., IN
Source
Review of Professional Management- A Journal of New Delhi Institute of Management, Vol 10, No 1 (2012), Pagination: 70-78Abstract
For employees in high pressure occupation like insurance and banking industry, discovering the equilibrium between life and work is an off-putting task. The present study tries to look at the awareness, attitude, perceived importance and formalization of the work-life balance (WLB) policies of employees in insurance and banking sector. In this research, stratified random sampling technique was applied and a sample size of 148 was taken; then the reliability statistics such Cronbach's alpha was used. Statistical techniques used to analyze data were descriptive statistics (Mean and Standard Deviation), chi square test, Analysis of Variance (ANOVA) and related procedures thereof like Multiple comparisons (Games-Howell test, Tukey HSD) and Levene Statistics for Test of Homogeneity of Variances. Findings suggest that employees perceive leave policies like maternity/paternity, and bereavement etc. as the most vital WLB policy. Also, the study finds that there were no formal procedures on work life balance policies in institutes. Income, gender and type of organization had statistically significant influence on the attitudes of employees towards implementation of these policies.Keywords
Attitude, Work-Life Balance, Employees, Insurance Companies, Banks.- Software Defined Networking a Comprehensive Study
Abstract Views :142 |
PDF Views:0
Authors
Neha Joshi
1,
Deepak Gupta
1
Affiliations
1 Computer Science & Engineering, NIT Arunachal Pradesh, Yupia 791112, IN
1 Computer Science & Engineering, NIT Arunachal Pradesh, Yupia 791112, IN
Source
Research Cell: An International Journal of Engineering Sciences, Vol 31, No SP (2019), Pagination: 38-43Abstract
.References
- Kreutz, Diego, et al. "Software-defined networking: A comprehensive survey." Proceedings of the IEEE 103.1: 14-76(2015).
- Xia, Wenfeng, et al. "A survey on software-defined networking." IEEE Communications Surveys & Tutorials 17.1: 27-51(2015).
- Software-Defined Networking (SDN) - Cisco. [Online]. Available:https://www.cisco.com/c/en_in/solutions/software-defined-networking/overview.html. [Accessed: 27-Nov-2018].
- "What Is Software-Defined Networking (SDN)? - Definition from WhatIs.com." SearchSDN, searchsdn.techtarget.com/definition/software-defined-networking-SDN (2013).
- Jain, Sushant, et al. "B4: Experience with a globally-deployed software defined WAN." ACM SIGCOMM Computer Communication Review. Vol. 43. No. 4. ACM, (2013).
- Burgess, Jan. "7 Advantages of Software Defined Networking." Ingram Micro Advisor, www.ingrammicroadvisor.com/data-center/7-advantages-of-software-defined-networking (2017).
- Kim, Hyojoon, and Nick Feamster. "Improving network management with software defined networking." IEEE Communications Magazine 51.2: 114-119(2013).
- Locale Differentials in Developmental Readiness of Private School Children
Abstract Views :172 |
PDF Views:0
Authors
Affiliations
1 Department of Human Development & Family Studies, Punjab Agriculture University, Ludhiana, Punjab, IN
1 Department of Human Development & Family Studies, Punjab Agriculture University, Ludhiana, Punjab, IN
Source
Indian Journal of Positive Psychology, Vol 10, No 3 (2019), Pagination: 156-161Abstract
Developmental readiness plays an important role in early childhood period and it sets the basis for future development. It is multi-faceted concept comprised of physical, cognitive, socio-emotional and self-help skills of children. Thus developmental readiness of children assures smooth and successful transition to formal schooling. The purpose of the present study was to assess the developmental readiness of private school children. The total sample of the study comprised of 200 children (i.e., 100 rural & 100 urban) of class- I randomly selected from private schools of rural and urban area. Self-structured Developmental readiness checklist was used to assess its different components.The results revealed that most of the rural and urban children had high level of skills across all domains. Locale differences showed that both rural children showed significantly better skills across various domains of developmental readiness than urban children except in cognitive skills and fine motor skills. Implications o fresearch for practice are also discussed in the present study.Keywords
Developmental Readiness, Cognitive Readiness, Gross and Fine Motor Readiness.- Assessing the Efficacy of a Life Skills Enrichment Program on Mitigating Peer Pressure among University Students
Abstract Views :134 |
PDF Views:0
Authors
Affiliations
1 Department of Human Development & Family Studies, Statistic & Physics Punjab Agricultural University, Ludhiana, Punjab, IN
2 Department of Mathematics, Statistic & Physics Punjab Agricultural University, Ludhiana, Punjab, IN
1 Department of Human Development & Family Studies, Statistic & Physics Punjab Agricultural University, Ludhiana, Punjab, IN
2 Department of Mathematics, Statistic & Physics Punjab Agricultural University, Ludhiana, Punjab, IN