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Capalash, Neena
- Characterization of Immobilized Laccase from γ-proteobacterium JB: Approach towards the Development of Biosensor for the Detection of Phenolic Compounds
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Authors
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1 Dept. of Microbiology, Dept. of Biotechnology, Punjab University, Chandigarh 160014, IN
1 Dept. of Microbiology, Dept. of Biotechnology, Punjab University, Chandigarh 160014, IN
Source
Indian Journal of Science and Technology, Vol 3, No 1 (2010), Pagination: 48-53Abstract
The two factors important when optimization of enzyme immobilization for the fabrication of biosensor are: activity and stability. The present study investigates the 2 factors when laccase was immobilized on various supports by different methods. Immobilization of partially purified laccase showed that enzyme expressed 100% activity when immobilized on the nitrocellulose membrane. pH and temperature optimum of immobilized laccase was 6.5 and 55°C respectively, when syringaldazine was used as a substrate. Immobilized laccase on nitrocellulose membrane was 100% stable at 4°C -30°C for three months. At 60°C enzyme showed 50% stability after 30 min. Immobilized laccase showed best response with syringaldazine which gave reaction even at 1 to 5μM concentration. Immobilized laccase gave response to catechol, catechin and L-methyl DOPA in the range of 40 to 90, 40 to 60, 30 to 70 µM respectively. ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate)) also showed response to the laccase from 0.1 to 0.2 mM.Keywords
Immobilization, Laccase, Nitrocellulose Membrane, γ-proteobacteriumFull Text
References
- Ahn MY, Dec JE and Bollag (2002) Treatment of 2,4- dichlorophenol polluted soil with free and immobilized laccase. J. Environ. Quality. 31,1509-1515.
- Bains J, Capalash N and Sharma P (2003) Laccase from a non melanogenic, alkalotolerant γ- proteobacterium JB isolated from industrial waste water drained soil. Biotechnol. Lett. 25, 1155-1159.
- Bourbonnais R, Paice MG, Freiermuth B and Borneman S (1997) Reactivities of various mediators and laccases with kraft pulp and lignin model compounds. Appl. Environ. Microbiol. 63, 4627-4632.
- Degryse E, Glandsdorff N and Picrard A (1978) A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. ArchivesMicrobiol. 117, 189-196.
- Duran N and Esposito E (2000) Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Appl. Catal. B Environ. 28, 83-99.
- Ghindilis AL, Gavrilova VP and Yaropolov AI (1992) Laccase-based biosensor for determination of polyphenols: determination of catechols in tea. Biosensor Bioelec. 7(2), 127-131.
- Gianfreda L and Xu F. Bollag (1999) Laccases: a useful group of oxidoreductive enzymes. Bioremediation. 3, 1-25.
- Givaudan A, Effosse A, Faure D, Potier P, Bouillant ML and Bally R (1993) Polyphenol. Polyphenol oxidase from Azospirillum lipoferum isolated from rice rhizosphere: evidence of laccase activity in nonmotile strains of Azospirillum lipoferum. FEMS Microbiol. Lett. 108, 205-210.
- Hullo MF, Moszer I, Danchin A and Martin-Verstraete (2001) Cot A of Bacillus subtilis is a copperdependent laccase. J. Bacteriol. 183, 5426-5430.
- Kumaran S and Morita M (1995) Application of a cholinesterase biosensor to screen for organophosphorous pesticides extracted from soil. Talanta. 42 (4), 649-655.
- Kuznetsov BA, Shumakovich GP, Koroleva OV and Yaropolov AI (2001) On applicability of laccase as label in the mediated and mediator less electroimmunoassay: effect of distance on the direct electron transfer between laccase and electrode. Biosensor Bioelectron. 16, 73 –84.
- Mateo JM, Gloria P, Fernandez-Lorente and Fernandez-Lafuente (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enz. Microb. Tech. 40, 1451–1463.
- Mazura MP, Krysinski A, Michota-Kamińska, Bukowska J, Rogalski G and Blanchard (2007) Immobilization of laccase on gold, silver and indium tin oxide by zirconium–phosphonate–carboxylate (ZPC) coordination chemistry. Bioelectrochem. 71,15–22.
- Sanchez-Amat A and Solano F (1997) A pluripotent polyphenol oxidase from the melanogenic marine Alteromonas sp. shares catalytic capabilities of tyrosinases and laccases. Biochem. Biophys. Res. Commun. 240, 787-792.
- Seok N, Hee–Yeon, Soo–Jeong S, Yun–Jeong, Leonwicz A and Ohga S (2008) Production of fungal laccase and its immobilization and stability. Fac. Agri. Kyushu Univ. 53 (1), 13–18.
- Sharma P, Goel R and Capalash N (2007) Bacterial laccases. World J. Microbiol. Biotechnol. 23, 823-832.
- Sharma SK, Sehgal N and Kumar A (2002) A quick and simple biostrip technique for detection of lactose. Biotechnol. Lett. 24, 1737–1739.
- Singh G, Ahuja N, Batish M, Capalash N and Sharma P (2008) Biobleaching of wheat straw-richsoda pulp with alkalophilic laccase from γ- proteobacterium JB: Optimization of process parameters using Response Surface Methodology. Biores. Technol. 99, 7472–7479.
- Singh G, Capalash N, Goel R and Sharma P (2007) A pH-stable laccase from alkali-tolerant γ- proteobacterium JB: Purification, characterization and indigo carmine degradation. Enz. Microb.Technol.41, 794-799.
- Tembe S, Inamdar S, Santosh H, Karve S F and D' Souza (2007) Electrochemical biosensor for catechol using agarose–guar gum entrapped tyrosinase. J. Biotechnol. 128, 80-85.
- Wen G, Zhang Y, Zhou Y, Shuang S, Chuan D and Martin M (2005) Biosensors for determination of galactose with galactose oxidase immobilized on eggshell membrane. Anal. Lett. 38,1519-1529.
- Xu F (2005) Applications of oxidoreductases: Recent progress. Industrial Biotechnol. 1, 38-50.
- Yaropolov AI, Skorobogat'ko SS and Varfolomeyev (1994) Laccase: Properties, catalytic mechanism, and applicability. Appl. Biochem. Biotechnol. 49, 257-280.
- Yinghui D, Qiuling W and Shiyu F (2002) Laccase stabilization by covalent binding immobilization on activated polyvinyl alcohol carrier. Lett. Appl. Microbiol. 35, 451-456.