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Nathani, Neelam M.
- Analysis of Community Structure and Species Richness of Protozoa-Enriched Rumen Metagenome from Indian Surti by Shotgun Sequencing
Abstract Views :359 |
PDF Views:133
Authors
Ravi K. Shah
1,
Amrutlal K. Patel
1,
Tejas M. Shah
1,
Krishna M. Singh
1,
Neelam M. Nathani
1,
Chaitanya G. Joshi
1
Affiliations
1 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
1 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
Source
Current Science, Vol 111, No 1 (2016), Pagination: 184-191Abstract
The present study applies metagenomics to characterize the diversity and relative abundance of protozoa residing in the rumen of Indian Surti buffalo (Bubalus bubalis). To increase protozoa representation in the metagenome, protozoa enrichment was performed by density sedimentation and confirmed by quantitative real time PCR. The highly enriched metagenome sample was subjected to shotgun sequencing by Ion Torrent PGM which resulted in 10,303,375 reads total-ling 1.6 gigabases. The taxonomic profile obtained by comparison with SILVA SSU database showed pre-dominance of the class Litostomatea (99.78%) fol-lowed by Coccidia (0.10%) and Aconoidasida (0.06%). At the genus level Isotricha (48.06%) followed by Polyplastron (9.90%), Dasytricha (9.87%) and Eudiplodinium (7.47%) were predominant. The taxo-nomic assignment based on protein coding regions showed discrepancy with the SSU-based assignments, possibly due to the absence of most eukaryotic ge-nomes in public databases. According to the SEED subsystems annotation database, genes for protein me-tabolism were the most abundant followed by genes for RNA metabolism, regulation and cell signalling. The present study offers a preliminary snapshot of di-versity, functional potential and relative abundance of protozoa within the Indian Surti buffalo rumen and al-so expands our knowledge of these unicellular eukary-otes present in the rumen ecosystem.Keywords
Buffalo Rumen, Metagenome, Protozoa Enrichment, Quantitative Real Time PCR, Shotgun Sequencing.Full Text
- Genome-Wide Analysis of a Potent Functional Dairy Starter Bacterium Streptococcus thermophilus MTCC 5460:A Comprehensive Study of its Dairy Niche Adaptive Features
Abstract Views :470 |
PDF Views:137
Authors
Jashbhai B. Prajapati
1,
Hemaxi P. Zala
1,
Neelam M. Nathani
2,
Manisha Sajnani
2,
Chaitanya G. Joshi
2
Affiliations
1 Department of Dairy Microbiology, Sheth MC College of Dairy Science, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
2 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
1 Department of Dairy Microbiology, Sheth MC College of Dairy Science, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
2 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, IN
Source
Current Science, Vol 113, No 12 (2017), Pagination: 2292-2297Abstract
Genomic analysis of Streptococcus thermophilus strain MTCC 5460, an isolate from market dahi (curd), revealed particular gene features that contributed towards its adaptation to a dairy-specific niche. The genome comprising 1.6 Mb, encoding 1809 genes, revealed the presence of genes involved in lactose/galactose utilization; well-developed proteolytic system including cell envelop proteinases and several transporters; and bacteriocin synthesis and competence proteins involved in defence mechanism, which help prevent food spoilage. The genome comprised genes for stress resistance property of the strain, contributing to its gut endurance and gene encoding formation of aroma compounds. Unlike pathogenic streptococci, genes for virulence property were absent in the genome. Overall, the study revealed features within the genome that enabled the organism to survive in a gastric environment and assisted in its interaction with the host microbiota and mucosa, thus, validating the strain as a potent functional dairy starter and a promising candidate for potential probiotic applications.Keywords
Dairy Starter, Genome, MTCC 5460, Probiotics.Full Text
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