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B. Thangabalan ¹, M. Salomi ¹, N. Sunitha ¹, S. Manohar Babu ¹

Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN

Abstract

A simple, fast and precise RP-HPLC method was developed for the quantification of Itraconazole in pure and pharmaceutical dosage form. The quantification was carried out using Dionex C18 4.6 X 250mm, 5μm enhanced polar selectivity column and mobile phase comprised of methanol and pH 7.5 potassium dihydrogen phosphate in the ratio of 40:60 and degassed under ultrasonication. The flow rate was 1.5ml/min and the effluent was monitored at 306nm. The retention time of Itraconazole was found to be 5.2 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Itraconazole was in the range of 200-600 μg/mL. The percentage recoveries of Itraconazole were 99.33% to 99.66% from the capsule formulation. The proposed method is suitable for determination of Itraconazole in pharmaceutical dosage form.

Keywords

Chromatography, Itraconazole, Method Validation.

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N. Sunitha ¹, L. Sindhura ¹, B. Thangabalan ¹, S. Manohar Babu ¹

Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN

Abstract

A new, rapid, highly sensitive, economical and accurate RP-HPLC method was developed for simultaneous estimation of Cefepime and Tazobactam in injection formulation. The separation was achieved by C18 column (150 × 4.6 mm, 5 μ particle size) with mobile phase consisting of phosphate buffer (pH 2.4, diluted with orthophosphoric acid), methanol and acetonitrile in the ratio of 90:5:5 v/v, using flow rate 1.1 mL/min and eluents monitored at 260 nm. The developed method was validated as per ICH guidelines for specificity, linearity, precision, accuracy, robustness, limit of detection and limit of quantification. The retention times of cefepime and tazobactam were 2.236 and 4.498 min respectively. The linearity was found to be in the range of 250-750 μg/mL and 31.25-93.75 μg/mL for cefepime and tazobactam sodium respectively, had regression coefficients (R2) 0.999. The proposed method was successfully applied for simultaneous estimation of both drugs in injection formulation.

Keywords

RP-HPLC, Cefepime, Tazobactam, Injection, Validation.

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B. Thangabalan ¹, Avinash Koya ¹, G. Chaitanya ¹, N. Sunitha ¹, S. Manohar Babu ¹

Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN

Abstract

A simple, fast and precise stability indicating RP - HPLC method was developed for the separation and quantification of Acamprosate in pharmaceutical dosage form. The quantification was carried out using Hypersil C18 4.6X150 mm, 5 μm enhanced polar selectivity column and mobile phase comprised of 0.2M Ammonium acetate and acetonitrile in proportion of ratio 40:60 and degassed under ultrasonication. The flow rate was 1mL/min and the effluent was monitored at 220 nm. The retention time of Acamprosate was 4.187min. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Acamprosate was in the range of 5 - 30 μg/mL. The percentage recoveries of Acamprosate was 99.00% from the tablet formulation. The stability - indicating capability was established by forced degradation experiments. The proposed method is suitable for determination of Acamprosate in pharmaceutical dosage form.

Keywords

Chromatography, Acamprosate, Forced Degradation, Method Development, Method Validation.

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N. Sunitha ¹, Y. Sujitha ¹, B. Thangabalan ¹, S. Manohar Babu ¹

Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN

Abstract

A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Ketorolac tromethamine and olopatadine HCl from bulk and formulations. Chromatographic separation was achieved isocratically on a Inertsil ODS C18 column (250×4.6 mm, 5 μ particle size) using a mobile phase 0.1 M Sodium di hydrogen orthophosphate: Acetonitrile in the ratio of 55:45. The flow rate was 1 ml/min and effluent was detected at 235nm. The retention time of Ketorolac and olopatadine were 2.507 min and 4.933 min. respectively. Linearity was observed in the concentration range of 4-24μg/ml and 12-72μg/ml for Ketorolac and olopatadine respectively with correlation coefficient of 0.999 for both the drugs. Percent recoveries obtained for ketorolac and olopatadine were 100.03% and 100.04%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Ketorolac and olopatadine from their combined dosage form.

Keywords

RP-HPLC Method, UV-VIS Detection, Keterolac and Olopatadine Ophthalmic Formulation.

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B. Thangabalan ¹, Ch. Sandhya ¹, N. Sunitha ¹, S. Manohar Babu ¹

Affiliations
1 Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.), IN

Abstract

A simple, fast and precise stability indicating RP - HPLC method was developed for the quantification of Memantine HCl in pure and pharmaceutical dosage form. The quantification was carried out using Intersil ODS C18 4.6 X 150mm, 5μm enhanced polar selectivity column and mobile phase comprised of 0.2M sodium dihydrogen phosphate&0.1M disodium hydrogen phosphate buffer of pH adjusted to 3 with orthophosphoric acid and Acetonitrile in proportion of ratio 50:50 and degassed under ultrasonication. The flow rate was 1ml/min and the effluent was monitored at 272nm. The retention time of Memantine HCl was found to be 3.337 min. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guidelines. Linearity of Memantine was in the range of 20 - 120 μg/mL. The percentage recoveries of Memantine were 99.66% to 102.5% from the tablet formulation. The stability - indicating capability was established by forced degradation experiments. The proposed method is suitable for determination of Memantine HCl in pharmaceutical dosage form.

Keywords

Chromatography, Memantine HCI, Method Validation, Forced Degradation Study.

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N. Sunitha ¹, P. K. Basavaraja ¹, B. N. Dhananjaya ¹

Affiliations
1 Department of Soil Science and Agricultural Chemistry, University of Agricultural Sciences, Gandhi Krishi Vigyan Kendra, Bangalore – 560 065, IN

Abstract

A field-experiment was conducted in salt-affected soils of Dodda Seebi tank command area of Tumkur district, Karnataka during rabi season of 2007 to study effect of gypsum, P level, borax and iron sulphate on beet ischolar_main tuber yield and nutrient uptake. Treatments included two main-plot treatments, viz., M0: Control (without gypsum) and M₁: gypsum application @ 9.0 t ha^-1 and eight sub-plot treatments, viz., S₁: Phosphorus @ 100 kg P₂O₅ ha^-1, S₂: Phosphorus @ 150 kg P₂O₅ ha^-1, S₃: S₁ + Borax @ 5 kg ha^-1, S₄: S₂ + Borax @ 5 kg ha^-1, S₅: S₁ + FeSO₅ @ 25 kg ha^-1, S₆: S₂ + FeSO₄ @ 25 kg ha^-1, S₇: S₃ + FeSO₄ @ 25 kg ha^-1 and S₈: S₄ + FeSO₄ @ 25 kg ha^-1. Recommended N and K were applied to all treatments. The experiment was laid out in split plot design with three replications. Beet ischolar_main, a salt-tolerant crop, was sown for testing its performance in salt-affected soils. Significantly higher tuber yield of 12.70 t ha^-1 was realized when the crop received gypsum @ 9.0 t ha^-1 compared to control (7.73 t ha^-1), besides higher nutrient uptake by the tubers. Among the nutrients, application of P at higher level (150 kg P₂O₅ ha^-1) plus recommended NK along with borax and iron sulphate realized higher tuber yield (15.72 t ha^-1) as well as nutrients uptake by tubers. Crop that received gypsum in combination with P at a higher level plus recommended NK, along with borax and iron sulphate, resulted in highest tuber yield (19.72 t ha^-1) and nutrient uptake.

Keywords

Salt-Affected Soil, Beet Root, Gypsum, Tuber Yield, Nutrient Uptake.

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N. Sunitha ¹, K. G. Manjunatha ²

Affiliations
1 Department of Chemistry, City Engineering College, Bangalore, Karnataka, IN
2 Department of Chemistry, Ghousia College of Engineering, Ramanagaram, Karnataka, IN

Abstract

Aluminum matrix composites (AMCs) are a range of advanced engineering materials that can be used for a wide range of applications within the aerospace, automotive, biotechnology, electronic and sporting goods industries. AMCs consist of a non-metallic reinforcement (SiC, Graphite etc.) incorporated into Aluminum matrix which provides advantageous properties over base metal (Al) alloys. These include improved abrasion resistance, creep resistance, dimensional stability, exceptionally good stiffness-to-weight and strength-to-weight ratios and better high temperature performance. Present paper involves the study of corrosion properties of aluminum metal matrix composites and unreinforced alloy. AMCs  is prepared using Silicon Carbide and Graphite. Al 6065 series alloy is used to prepare composite with varied percentage of SiC (0, 2, 4%) and hybrid composite with equal amount of SiC and Graphite is prepared by liquid metallurgy technique using vortex method. The corrosion studies were carried out by weight loss method by immersing the specimen for 24 hrs., in various corrosion medium such as acid chloride, acid sulphate and neutral mediums. Results indicate that hybrid composite shows more resistance to corrosion.

Keywords

Aluminum, Composites, Silicon Carbide, Graphite, Corrosion Rate.

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