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A. Vinoth ¹, V. Balakrishnan ¹, R. Kalaivani ¹, P. Madhanraj ², N. Nadimuthu ³

Affiliations
1 Department of Biotechnology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN

Abstract

Oil pollution is worldwide major problem in every environment and hence the world is need of a perfect solution for prevention or recovery so, the present investigation was carried out to detect the spent lubricant oil degradation potentional of indigenous fungi in lab level by measuring the growth and analysis the treated sample using FTIR. Soil sample was randomly collected in the different location at railway tracks in Thanjavur junction soil sample are serially and 10^-2 is used for plating technique in PDA medium. After incubation the isolates were obtained and they are Aspergillus terreus, Aspergillus flavus and Aspergillus niger. Oil degrading ability was detected supplying raw spent oil in PDA medium and incoprporated in PD broth at the concentration of (5%.10%, 15% and 20%), detection shows that no fungal cored use raw oil as source of nutrient. In PDA plate's visual detection of zone due to the degradation was noticed. It was maximum by Aspergillus flavus follwed by Aspergillus terreus and Aspergillus niger. In PD broth supplemented with different spent oil concentration (5%,10%,15% and 20%), after 14 days of incubation (30°C), as a visual the biomass of each fungal culture was determined the biomass of different species of fungi in different concentration oil showed growth but with variation the higher mycelial biomass was recorded by Aspergillus flavus. The FT-IR analysis results shows that is major difference in the peak formation between the tested samples which shows that fungal species has utilized or degraded the oil hydrocarbon. Differently as per their metabolize activity.

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P. Madhanraj ¹, S. Manorajan ², N. Nadimuthu ³, A. Panneerselvam ⁴

Affiliations
1 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Biology Education Centre, Uppsala University, SE-752 36 Uppsala, SE
3 Dept. of Plant Science, Avvaiyar Government College for Women, Karaikal – 609 602, Pondicherry, U.T., IN
4 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613 503, Thanjavur (Dt.), Tamilnadu, IN

Abstract

In seasonally inundated sediments of Manianthivu of Nagapattinam district, southeast coast of India, a total number of 24 species belonged to 5 genera were recorded. They were assignable to Phycomycete (1 species) and Deuteromycetes (23 species). They exhibited spatio-temporal changes. The genus Aspergillus was constituted by the maximum of 19 species followed by Trichoderma (2 species). All other genera (Absidia, Botrytis and Fusarium) were represented by one species each. The soil characteristics such as pH (8.1 to 8.9), electrical conductivity (0.11 to 1.28 dSm^-1), cation exchange capacity (8.01 to 9.5 c.mol proton⁺/kg), organic carbon (0.05 to 0.18%), available nitrogen (0.012 to 0.018%), available phosphorus (0.002 to 0.005%), available potassium (0.013 to 0.047 ppm), available zinc (0.39 to 0.57 ppm), available iron (2.12 to 2.83 ppm), available copper (0.19 to 0.31 ppm), available manganese (1.41 to 1.52 ppm), calcium (3.2 to 5.2 mg/kg), magnesium(3.2 to 3.9 mg/kg), sodium (0.52 to 1.22 mg/kg) and potassium (0.02 to 0.07 mg/kg) were also showed variation during different seasons. The investigation was carried out by collections and examination of sediment samples during January 2007 - December 2008, at seasonal intervals.

Keywords

Fungi, Soil Characteristics, Inundated Sediment.

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P. Sumithra ¹, T. Viji ¹, P. Madhanraj ², N. Nadimuthu ³

Affiliations
1 Department of Microbiology, Srimad Andavan Arts and Science College, Tiruchirappalli - 620 005, Tamil Nadu, IN
2 Indian Biotrack Research Institute, Thanjavur, Tamil Nadu, IN
3 P.G. Department of Plant Science, Avvaiyar Government College for Women, Karaikal 609 602, Puducherry U.T., IN

Abstract

The soils collected from a backwater environment at Vellapallam in Nagapattinam district of Tamilnadu state along the east coast of India during May 2013 were subjected to the examination of fungi on PDA medium and physicochemical characteristics. The culturable propagules of the fungi were in the range from 6.98 to 106 x 10² CFU/g and the species diversity (number of species) was from 5 to 9.

The pH of the soil was in the range from 7.9 to 8.9; electrical conductivity from 0.04 to 1.31 dSm^-1; cation exchange capacity from 8.01 to 10.11 c.mol proton⁺/kg; organic carbon from 0.04 to 0.21%; available nitrogen from 0.011 to 0.22%; available phosphorus from 0.001 to 0.006%; available potassium from 0.011 to 0.057 ppm; available zinc from 0.36 to 0.57 ppm; available iron from 2.01 to 2.91 ppm; available copper from 0.14 to 0.34 ppm; available manganese from 1.22 to 1.53 ppm; calcium from 3.1 to 5.7 mg/kg; magnesium from 3.1 to 4.2 mg/kg; sodium from 0.29 to 1.73 mg/kg; and potassium 0.01 to 0.08 mg/kg. Among them electrical conductivity and the Organic Matter showed significant positive correlation with the total fungal population.

All the fungal species that were isolated from soils were tested against F. semitectum, a known soil borne fungal pathogen, by dual culture and food poisoning methods. Among them Trichoderma koeningii showed promising activity against the pathogenic fungus for the maximum both in dual culture and in food poisoning techniques. The inhibition was 61.3% at 20% concentration. As the antagonistic fungus was isolated from the environment that showed typical marine conditions, the isolate could better serve as biocontrol agent to control the soil born pathogen, F. semitectum in saline soil crop fields.

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P. Madhanraj ¹, K. Ramanathan ², N. Arun ³, N. Nadimuthu ⁴, A. Panneerselvam ⁵

Affiliations
1 Department of Microbiology, Thanthai Hans Roever College, Perambalur-621212, IN
2 Department of Bioinformatics, Thanthai Hans Roever College, Perambalur-621212, IN
3 Department of Biochemistry, Thanthai Hans Roever College, Perambalur-621212, IN
4 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
5 Department of Botany and Microbiology, A.V.V.M Sri Puspam College, Poondi, Thanjavur–613503, Tamil Nadu, IN

Abstract

We have been investigating that the atomic study of Fusarium incarnatum with the utilization of Bioinformatics tools. We have been involved in the insilico study on the plant pathogen the Fusarium incarnatum. The involvement of Nucleotides and atoms in this species can be identified with the help of Bioinformatics tools. We can also involved in the analysis of amino acids which are present in this species and calculated the start and stop codons in different frames.

Keywords

Fusarium incarnatum, Bioinformatics Tools, Nucleotides, Atomic Study, Amino Acids, Frame Analysis.

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P. Madhanraj ¹, N. Nadimuthu ², A. Panneerselvam ³

Affiliations
1 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur, Tamil Nadu, IN
2 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
3 P.G. and Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt.), Tamilnadu, IN

Abstract

A total number of 325 colonies were isolated by plating method from the soils of Casurina bioshield plantation at Naluvedhapathy (Nagapattinam Dt.), along south east coast of India. There were 19 species belonged to 4 genera with Aspergillus as the dominant genus, represented by more number of species (14 species), followed by Fusarium and Trichoderma (2 species each). Population density varied from 9.7 to 17.7 CFU/g and the species diversity from 6 to 10 species during different stations. Physico-chemical analyses of the soils revealed the following features: pH 7.9 to 8.8, electrical conductivity 1.06 to 1.31 dSm^-1, cation exchange capacity 8.67 to 10.11 c.mol proton⁺/kg, organic carbon 0.04 to 0.21%, available nitrogen 0.011 to 0.022%, available phosphorus 0.002 to 0.005%, available potassium 0.013 to 0.051 ppm, available zinc 0.47 to 0.57 ppm, available iron 2.13 to 2.90 ppm, available copper 0.22 to 0.31 ppm, available manganese 1.38 to 1.53 ppm, calcium 3.1 to 5.1 mg/kg, magnesium 3.2 to 4.1 mg/kg, sodium 0.23 to 0.79 mg/kg and potassium 0.02 to 0.08 mg/kg, during different seasons.

Keywords

Soil Fungi, Bioshield Plantation, Soil Characteristics, Mycoecology.

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