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Noviana Dwi Lestari ¹, Wahyu Isnia Adharini ¹, Widodo ¹, Sri Rahayu ¹, Hideo Tsuboi ², Yoga Dwi Jatmiko ¹, Muhaimin Rifa’i ¹

Affiliations
1 Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, ID
2 Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550,, JP

Abstract

This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-γ and TNF-α, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels ≥ 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-γ and TNF-α expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-γ and TNF-α. Furthermore, dose 1 affected to decrease in the IFN-γ expression while dose 3 decreased the expression of TNF-α. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-γ and TNF-α in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.

Keywords

Albumin, Diabetes Mellitus, IFN-γ. Moringa oleifera, TNF-α,.

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Sapti Puspitarini ¹, Nashi Widodo ², Sri Widyarti ², Yoga Dwi Jatmiko ², Muhaimin Rifa’i ²

Affiliations
1 Doctoral Program, Biology Department, Science and Mathematics Faculty, Brawijaya University, Malang,, ID
2 Biology Department, Science and Mathematics Faculty, Brawijaya University, Malang,, ID

Abstract

A combination of herbals medicines is an alternative treatment choice for developing anticancer therapy because of its benefits, active compounds, and non-toxic side effect. This study investigates the anticancer and antioxidant activity of Phyllanthus urinaria and Curcuma longa water extract combination. The analysis of their bio-active components was done using LC-HRMS. The biological activity prediction was made using PassOnline and Cytoscape. Anticancer activity, apoptosis, and cell cycle assay were tested on treated T47D breast cancer cells line with the combination herbal extract and individual herbal extract compare to the untreated and cisplatin-treated control cells. This study also determined the antioxidant activity and phenolic and flavonoid total assay. The result showed that these herbs' compounds were predicted to have a biological function in cancer treatment. The herbal combination has efficiency inducing apoptosis with more than 50% compared to the individual herbal treatment and untreated control cells. Cell cycle analysis shows these herbals affect cell cycle arrest at G2/M phase compared to the control cells. The antioxidant activity and total phenol and flavonoid of P. urinaria and C. longa combination positively correlate with the anticancer activity result. Compusyn test results on cell toxicity parameters showed that the combination of both herbs had an additive effect (C = 1). Although this combination has no synergism effect, the water extract of P. urinaria and C. longa combination can be an anticancer agent.

Keywords

Additive effect; Anticancer; Antioxidant activity; Curcuma longa; Herbal formulation; In silico; In vitro; Phyllanthus urinaria.

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