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Saluja, Manmeet Singh
- Evaluation of Effect of Rotenone on C6 Glial Cells by Comet Assay
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Authors
Affiliations
1 Dept. of Pharmacognosy, SRM University Tamil Nadu, IN
2 Department of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, IN
1 Dept. of Pharmacognosy, SRM University Tamil Nadu, IN
2 Department of Pharmacy, Suresh Gyan Vihar University, Jaipur, Rajasthan, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 2, No 1 (2010), Pagination: 59-62Abstract
Present study was performed to explore the action of rotenone on C6 glial cells. Rotenone is environmental pesticide and could specifically cause damage to dopaminergic neurons. It is well often used to make experimental models of Parkinsons disease. We have explored the effect of rotenone on glial cells at different concentrations and time points. Two time points were selected prior to their doubling time. Different cytotoxic parameters were done to investigate the effect of rotenone on cell viability. Significant decrease in cell viability was observed at increasing concentration of rotenone in time dependent manner. We performed single cell gel electrophoresis (comet assay) to investigate the DNA damage after rotenone treatment. Results found showed rotenone caused apoptotic death of glial cells in In Vitro. Our study could contribute in the concept of glia degeneration and helps in further explorations of better therapeutics for neurodegenerative diseases.
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- Hepatoprotective Activity of Stem Bark of Nyctanthes arbor-Tristis linn
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Authors
Affiliations
1 Columbia College of Pharmacy, Raipur-493111, C.G., IN
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur 492010, C.G., IN
3 TIT College of Pharmacy, Bhopal-462001, M.P., IN
1 Columbia College of Pharmacy, Raipur-493111, C.G., IN
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur 492010, C.G., IN
3 TIT College of Pharmacy, Bhopal-462001, M.P., IN