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Prabhudutta Panda ¹, Arpita Ghosh ¹, Maitrayee Panda ¹

Affiliations
1 College of Pharmaceutical Sciences, Mohuda, Berhampur-760002, Orissa, IN

Abstract

The present study was carried out to elucidate the potential of methanol extract of Eupatorium odoratum L (Family- Asteraceae) leaves on antiinflammatory and wound healing effects using various animal models. The dried, powdered leaves of E. odoratum were extracted successively with petroleum ether (60-80°C), chloroform and methanol in soxhlet apparatus. The methanol extract (yield 23.6 % w/w with respected to dry powdered plant material) was selected for all experimental procedure. Anti-inflammatory effect were investigated by employing the carrageenan induced rat paw edema test in adult Wister albino rats and the wound healing activity in Swiss Albino rats. Results were revealed that the methanol extract was found significant anti-inflammatory effect (P<0.001) at the dose levels of 100,200 and 400 mg/kg, orally in mice. Ointment and gel was prepared from the methanol extract of E. odoratum and tested for pH, viscosity, spreadability, drug contents uniformity. The formulations were evaluated for its acute skin irritancy, wound healing activity in Swiss Albino rats. The formulations did not produce any skin irritation for about a week when applied over the skin. The formulations and the normal methanol extracts of E. odoratum showed significant (P<0.001) wound healing activity by excision wound model and comparable with that of the reference standards and control bases. The measurement of the wound areas were taken on 3^rd, 6^th, 9^th, 12^th, 15^th and 18^th days and the percentages of wound closures were calculated. It concludes that, methanol extract possessed remarkable anti-inflammatory and wound healing activity effect on animal models.

Keywords

Eupatorium odoratum Leaves, Methanol Extract, Anti-Inflammatory, Wound Healing.

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Prabhudutta Panda ¹, Maitrayee Panda ², Siva Shankar Nayak ², Durga Prasad Panda ²

Affiliations
1 Department of Pharmaceutics and Formulation, Development College of Pharmaceutical Sciences, Mohuda, Berhampur, Orissa, IN
2 College of Pharmaceutical Sciences, Mohuda, Berhampur-760002, Orissa, IN

Abstract

The pharmacological evaluation of the chloroform extract of Pandanus fascicularis Lamk. leaves was found that it is having antinociceptive and anti-inflammatory properties, and contains the phytoconstituents viz., steroids, terpenoids, flavonoids, saponins and tannins. Therefore tablets were formulated with the extract by wet granulation method. The mechanical properties of the tablets like crushing strength and friability were assessed. The drug release properties were evaluated by using in-vitro disintegration and dissolution methods. There were statistically significant differences (p<0.01) in the crossing strength friability ratio (CSFR) of the tablets. The differences in drug release depend on the types and concentration of the excipients and the binders employed in the formulation. PVP, gelatin and corn starch were used in different concentrations i.e. 1% w/w and 4% w/w with purified water as binder solutions, for the preparation of tablets. The ranking of crushing strength values for tablets was PVP > gelatin > corn starch. The CSFR values also increased with increasing compression force and concentration of binders employed. The ranking of CSFR values for tablets was PVP > gelatin > corn starch. The dissolution time and t80 (time required for 80% release of the extract) were studied with Double beam UV-VIS Spectrophotometer at 271 nm. The ranking of disintegration and dissolution times exhibited by tablets was PVP >gelatin > cornstarch. There was no change in mechanical and drug release properties of the tablets after storage at temperature of 30±2 0C and relative humidity of 75±5% for twelve months.

Keywords

Pandanus fascicularis Lamk. Leaves, Chloroform Extract, Tablets, In-Vitro Evaluation.

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