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Rai, Mahendra
- Biological and Phylogenetic Analyses, Evidencing the Presence of Gnomoniopsis Sp. in India, Causing Canker of Chestnut Trees: a New Report
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Authors
Source
Indian Forester, Vol 139, No 1 (2013), Pagination: 37-42Abstract
In India the chestnut population is confined to the Northern part of the country particularly in Jammu and Kashmir. Twenty seven isolate of genus Gnomoniopsis were recovered in pure culture from infected chest nut trees growing in different places of Jammu and Kashmir. The morphological characteristics and the phylogeny of these isolates proved it to be Gnomoniopsis. The molecular identification of the isolates was carried out by rDNA based ITS markers, using ITS1, 5.8s and ITS2 for amplification and these rDNA regions were used to define the isolates as Gnomoniopsis. It is a first report of occurrence of Gnomoniopsis sp. from India.Keywords
Chestnut, Gnomoniopsis Sp., Rdna, Its, Phylogeny.
- Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn
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Authors
Eliana Marcela Soto Rueda
1,
Rodrigo Andrés Restrepo
1,
Nelsy Loango
1,
Beatriz Restrepo
1,
Patricia Landázuri
1,
Mahendra Rai
2
Affiliations
1 Universidad del Quindío, Quindío, CO
2 Department of Biotechnology, SGB Amravati University, Amravati - 444602, Maharashtra, IN
1 Universidad del Quindío, Quindío, CO
2 Department of Biotechnology, SGB Amravati University, Amravati - 444602, Maharashtra, IN
Source
Indian Journal of Science and Technology, Vol 12, No 1 (2019), Pagination: 1-6Abstract
Objective: To evaluate the anticancer and cytotoxic activity of the Origanum majorana and also to test its antioxidant potential. Methods/Statistical Analysis: The in vitro anticancer and cytotoxic activity of the Polar (PF) and Non-Polar Fraction (NPF) obtained from a total Ethanolic Extract (EE) of O. majorana were evaluated using the murine myeloma cell lines Sp2/O-Ag14 (ATCC: CRL-1581) and VERO-ATCC-CCL81. The cell viability was measured with the Colorimetric Quantification System of the Transmittance Index (CQSTI) method; a simple ANOVA of the CQSTI values of each cell line treated with the different extracts was carried out. Findings: The preliminary phytochemical analysis determined the presence of tannins, flavonoids and terpenes in the fractions. EE and PF showed a strong DPPH free radical scavenging capacity, 88,8 and 85.9% uptake, respectively. EE and PF displayed promising anti anticancer activity for both cell lines, so, EE, PF and NPF concentrations that produced 50% cell viability included 5.7 mg/mL, 5.8 mg/mL and 35.8 mg/mL, respectively. Application/Improvements: For the first time it is shown that the PF from O. majoranaa low concentrations affected myeloma cells but not VERO cells. These results indicate that this fraction has an anticancer activity. In addition, our results demonstrated that the PF has a high phenolic content, this could be related to its cytotoxic activity.Full Text
References
- Alok SA, Kumar S, Verma A, Kumar M, Mahor A, Sabharwal M. Herbal antioxidant in clinical practice: A review. Asian Pacific Journal of Tropical Biomedicine. 2014; 4(1):78–84. https://doi.org/10.1016/S2221-1691(14)60213-6
- Singh A, Singh S, Mohan PS. Role of medicinal plants for health perspective: Special reference to antioxidant potential. The Journal of Chemical Biology and Therapeutics. 2016; 1(2):1–5. https://doi.org/10.4172/2572-0406.1000106
- Karadeniz A, Çinbilgel I, Gün SŞ, Çetin A. Antioxidant activity of some Turkish medicinal plants. Natural Product Research. 2015; 29(24):2308–12. https://doi.org/10.1080/14 786419.2015.1005618 PMid:25649168
- Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines (Basel). 2018; 5(3):1–16. https://doi.org/10.3390/medicines5030093
- Hamdy MH, Mohamed Khaled AS, Abdel-Hamed S, Khalela I. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (O.majorana L.) extracts. Industrial Crops and Products. 2013; 43:827–31. https://doi.org/10.1016/j.indcrop.2012.08.029
- Karker M, Falleh H, Msaada K, Smaoui A, Abdelly C, Legault J, Ksouri R. Antioxidant, anti-inflammatory and anticancer activities of the medicinal halophyte Reaumuriavermiculata. Experimental and Clinical Sciences Journal. 2016; 15:297–307.
- Wamidh HT, Adel MM. Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scientia Pharmaceutica. 2010; 78(1):33–45. https://doi.org/10.3797/scipharm.0912-11 PMCid:PMC3002826
- Hajlaoui H, Mighri H, Aouni M, Gharsallah N, Kadri A. Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase properties of Tunisian O. majorana L. essential oil. Microbial Pathogenesis. 2016; 95:86–94. https://doi.org/10.1016/j.micpath.2016.03.003
- Zlotek U. Effect of jasmonic acid and yeast extract elicitation on low-molecular antioxidants and antioxidant activity of marjoram (O. majorana L). Acta Scientiarum Polonorum Technologia Alimentaria. 2017; 16(4):371–7. https://doi.org/10.17306/J.AFS.0505
- Olfa B, Mariem A, Salah AM, Mouhiba BA. Chemical content, antibacterial and antioxidant properties of essential oil extract from Tunisian O. majorana L. cultivated under saline condition. Pakistan Journal of Pharmaceutical Sciences. 2016; 29(6):1951–8.
- Abdel-Massih RM, Fares R, Bazzi S, El-Chami N, Baydoun E. The apoptotic and anti-proliferative activity of O. majorana extracts on human leukemic cell line. Leukemia Research. 2010; 34(8):1052–6. https://doi.org/10.1016/j.leukres.2009.09.018
- Al Dhaheri Y, Eid A, Abu Qamar S, Attoub S, Khasawneh M. mitotic arrest and Apoptosis in Breast Cancer Cells induced by O.majorana extract: Upregulation of TNF-a and downregulation of survivin and mutant p53. Tizi-Ouzou, Algeria. PLoS ONE. 2013; 8(2):1– 14. https://doi.org/10.1371/journal.pone.0056649 PMCid:PMC3579842
- Fathy SA, Emam MA, Abo Agwa SH, Abu Zahra FA, Youssef FS, Sami RM. The antiproliferative effect of Origanummajorana on human hepatocarcinoma cell line: Suppression of NF-κB. Cellular and Molecular Biology. 2016; 62(10):80–4.
- Bilbao MR. Análisis fitoquímico preliminar: química de los productos naturales. Universidad del Quindío. Armenia; 1997. p. 1–183
- Sanabria GA. Análisis fotoquímico preliminar: metodología y su aplicación en la evaluación de cuarenta plantas de la familia Compositae. Universidad Nacional de Colombia; 1983.
- Gunjan G, Raj kumar V, Ashok KR, Lazar M. Therapeutic potential of polar and non-polar extracts of Cyanthillium cinereum In Vitro. Evidence Based Complementary and Alternative Medicine; 2009. p. 1–10.
- Carneiro BR, Andrade LM, Gagioti SM, Barros F de M, Andrade PM. A simple method to measure cell viability in proliferation and cytotoxicity assays. Brazilian Oral Research. 2009; 23(3):255–62. https://doi.org/10.1590/S1806-83242009000300006
- Schulz H, Baranska M, Baranski R. Potential of NIR-FT-Raman spectroscopy in natural carotenoid analysis. Biopolymers. 2005; 77(4):212–21. https://doi.org/10.1002/bip.20215
- Hossain MB, Brunton NP, Patras A, Tiwari B, O’Donnell CP, Martin-Diana AB, Barry-Ryan C. Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanummajorana L.) using response surface methodology. Ultrasonics Sono Chemistry. 2012; 9(3):582–90. https://doi.org/10.1016/j.ultsonch.2011.11.001
- Vági E, Rapavi E, Hadolin M, Vásárhelyiné Perédi K, Balázs A, Blázovics A, Simándi B. Phenolic and triterpenoid antioxidants from Origanummajorana L. herb and extracts obtained with different solvents. Journal of Agricultural and Food Chemistry. 2005; 53(1):17–21. https://doi.org/10.1021/jf048777p
- Hazem SE, Armentano MF, Carmosino M, Bufo SA, De Feo V, Camele I. Cytotoxic activity of Origanumvulgare L. on hepatocellular carcinoma cell line HepG2 and evaluation of its biological activity. Molecules. 2017; 22(9).
- Erdogana A, Ozkana A. Investigatıon of antioxıdative, cytotoxic, membrane-damaging and membrane-protective effects of the essentıal oil of Origanummajorana and its oxygenated monoterpene component linalool in human-derived Hep G2 cell line. Iranian Journal of Pharmaceutical Research. 2017; 16:24–34.
- El-Ashmawy IM, el-Nahas AF, Salama OM. Protective effect of volatile oil, alcoholic and aqueous extracts of Origanummajorana on lead acetate toxicity in mice. Basic and Clinical Pharmacology and Toxicology. 2005; 97(4):238–43. https://doi.org/10.1111/j.1742-7843.2005.pto_136.x
- Khaleel FM, Hameed SH, Taghreed M. In vitro cytotoxic effect of aqueous extract of O. on AMN-3 cell line. Journal of Natural Sciences Research. 2016; 6(16):1–4.