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Udaya Kumar, M.
- Leaf Surface Wax Composition of Genetically Diverse Mulberry (Morus sp.) Genotypes and its Close Association with Expression of Genes Involved in Wax Metabolism
Abstract Views :461 |
PDF Views:154
Authors
H. M. Mamrutha
1,
K. N. Nataraja
2,
N. Rama
2,
D. K. Kosma
3,
T. Mogili
4,
K. Jhansi Lakshmi
5,
M. Udaya Kumar
2,
M. A. Jenks
6
Affiliations
1 Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
2 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
3 Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, US
4 Central Sericultural Research and Training Institute, Mysuru 570 008, IN
5 Central Sericultural Germplasm Resources Centre, Thally Road, Hosur 635 109, IN
6 Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, US
1 Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
2 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
3 Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, US
4 Central Sericultural Research and Training Institute, Mysuru 570 008, IN
5 Central Sericultural Germplasm Resources Centre, Thally Road, Hosur 635 109, IN
6 Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, US
Source
Current Science, Vol 112, No 04 (2017), Pagination: 759-766Abstract
Silkworm (Bombyx mori), the primary producer of silk, has strong feeding preference for most turgid and hydrated mulberry leaves. In a previous study, we showed positive correlation between moisture retention capacity (MRC) of the mulberry leaf and leaf surface wax amount. In the present study, we examined wax constituents in genotypes that exhibited a wide range of leaf surface wax amount and MRC. Gas chromatographic analysis revealed that acids, alkanes, aldehydes, primary alcohols, iso-alkanes, triterpenoids, esters were among mulberry waxes identified and the major being alkanes. The highest total leaf wax amount was 1006.8 μg dm-2 in the V1 genotype whereas S-36 had the least wax at 436.9 μg dm-2. The alkanes were dominated by those having 25, 27, 29, 31 and 33 carbons, with C31 compounds being the most abundant. RNA-blot hybridization performed with 13 wax biosynthesis associated genes using heterologous probes revealed a close association between surface wax composition and expression levels of genes associated with wax elongation (CUT1, KCS1). The association was also established with homologous probes of KCS1, WAX2, CUT1 and LTP1-like genes. In summary, regulation of early wax precursor metabolism is a likely contributor to the variation observed in leaf wax composition in mulberry.Keywords
Cuticular Wax, Moisture Retention Capacity, Mulberry, Silkworm, Wax Genes.Full Text
- Characterization of Finger Millet (Eleusine coracana L.) Recombinant Inbred Lines for Total Biomass
Abstract Views :289 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology and Crop Improvement, College of Horticulture (U.H.S.Campus), G.K.V.K., Bengaluru (Karnataka), IN
2 College of Horticulture (U.H.S. Campus), G.K.V.K., Bengaluru (Karnataka), IN
3 College of Horticulture, Sirsi, Uttara Kannada (Karnataka), IN
4 University of Agricultural Sciences, Bengaluru (Karnataka), IN
1 Department of Biotechnology and Crop Improvement, College of Horticulture (U.H.S.Campus), G.K.V.K., Bengaluru (Karnataka), IN
2 College of Horticulture (U.H.S. Campus), G.K.V.K., Bengaluru (Karnataka), IN
3 College of Horticulture, Sirsi, Uttara Kannada (Karnataka), IN
4 University of Agricultural Sciences, Bengaluru (Karnataka), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 2 (2017), Pagination: 311-314Abstract
Crop productivity is very much dependent on the biomass and its partition to economic parts in the plant system. In the present study, 150 recombinant lines were characterized for variability in total biomass. The mapping population was classified on the basis of biomass as low and high biomass types. Except SLA, other biometric traits varied significantly. Assessment of contribution of physiological traits other than the canopy cover that determine the variability in biomass is best done when genotypes with similar leaf area but differing in biomass are compared. All the traits varied significantly between low and high biomass types. To know the interrelationship among traits that contribute to the total biomass of the plant was also ascertained through correlation studies. A positive correlation was observed between various ischolar_main, shoot associated traits and the total biomass but ischolar_main to shoot ratio showed a negative relationship with biomass. Apart from ischolar_main and shoot dry weight, a strong positive correlation was observed between the leaf area and the biomass suggesting that the photosynthetic surface area contributes significantly to the biomass production.Keywords
Biomass, Finger Millet, Mapping Population.
References
- Araus, J. L., Slafer, G. A., Reynolds, M. P. and Royo, C. (2002). Plant breeding and drought in C3 cereals: What should We Breed For? Ann. Bot., 89 : 925-940.
- National Research Council (1996). Lost crops of Africa; Volume I Grains. National Academy Press, Washington, D.C., U.S.A.
- Passioura, J. B. (1982). The role of ischolar_main system characteristics in the drought resistance for crop plants. In: Drought resistance in cereals crops with emphasis on rice, IRRI, Los Bonos, Philippines, 71-82.
- Richards, R.A., Rebetzke, G.J., Condon, A.G. and Van Herwaarden, A.F. (2002). Breeding opportunities for increasing the efficiency of water use and crop yield in temperate cereals. Crop Sci., 42:111-121.
- Physiological Characterization of Finger Millet (Eleusine coracana L.) for Drought Tolerance
Abstract Views :257 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology and Crop Improvement, College of Horticulture (U.H.S. Campus), G.K.V.K., Bengaluru (Karnataka), IN
2 College of Horticulture, Sirsi, Uttara Kannada (Karnataka), IN
3 College of Horticulture (U.H.S. Campus), G.K.V.K., Bengaluru (Karnataka), IN
4 University of Agricultural Sciences, Bengaluru (Karnataka), IN
1 Department of Biotechnology and Crop Improvement, College of Horticulture (U.H.S. Campus), G.K.V.K., Bengaluru (Karnataka), IN
2 College of Horticulture, Sirsi, Uttara Kannada (Karnataka), IN
3 College of Horticulture (U.H.S. Campus), G.K.V.K., Bengaluru (Karnataka), IN
4 University of Agricultural Sciences, Bengaluru (Karnataka), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 2 (2017), Pagination: 403-409Abstract
A field experiment was conducted to characterize finger millet recombinant inbred lines (RILs) for drought tolerance. A set of 150 RILs with two parents IE 2912 and IE 2885 used were used to characterize for drought tolerance traits such as total leaf area, ischolar_main length, ischolar_main volume, moisture retention capacity (MRC) and SPAD chlorophyll reading (SCMR). Parent line IE 2912 was superior to IE 2885 for all the traits and both parents differed significantly for all traits except MRC and SCMR. In mapping population, ischolar_main traits, leaf traits, showed traits showed normal distribution around the mean and showed continuous variation indicating their quantitative nature. Since identified parental lines and mapping population developed are differing significantly they can be utilized in identifying markers linked to drought traits.Keywords
Finger Miller, RIL, Drought Tolerance, SCMR.References
- Araus, J.L., Slafer, G.A., Reynolds, M.P. and Royo, C. (2002). Plant breeding and drought in C3 cereals: What should We Breed For? Ann. Bot., 89: 925-940.
- Mohan, M., Nair, S., Bhagwat, A., Krishna, T.G., Yano, M., Bhatia, C.R. and Sasaki, T. (1997). Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol. Breed, 3 : 87-103.
- National Research Council (1996). Lost crops of Africa; Volume I Grains. National Academy Press, Washington, D.C., U.S.A.
- O’Leary, M.H. (1988). Carbon isotope in photosynthesis. Bio Sci., 38 : 325-336.
- Richards, R.A., Rebetzke, G.J., Condon, A.G. and Van Herwaarden, A.F. (2002). Breeding opportunities for increasing the efficiency of water use and crop yield in temperate cereals. Crop Sci., 42 : 111-121.
- Wu, K., Jones, R., Danneberger, L. and Scolnik, P. A. (1994). Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res., 22 : 3257-3258.