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Phani Kumar, M.
- Restriction Fragment Length Polymorphism (RFLP) Application in DNA Typing for Crime Investigation
Abstract Views :251 |
PDF Views:0
Authors
Affiliations
1 Department of Biochemistry, College of Medicine, Al-Jouf University 2014, Sakaka, Al-Jouf, Kingdom of Saudi Arabia, SA
2 Department of Biotechnology & Molecular Biology, BioAxis DNA Research Centre, Hyderabad 500 037, IN
1 Department of Biochemistry, College of Medicine, Al-Jouf University 2014, Sakaka, Al-Jouf, Kingdom of Saudi Arabia, SA
2 Department of Biotechnology & Molecular Biology, BioAxis DNA Research Centre, Hyderabad 500 037, IN
Source
Indian Journal of Forensic Medicine & Toxicology, Vol 4, No 1 (2010), Pagination: 79-82Abstract
DNA typing is on the rise in its use as an identification aid for forensic scientists. DNA typing have opened a new frontier in forensic analysis. Until 1985 all polymorphic immunological and biochemical markers employed to identify biological samples in criminal cases had limited applicability. The way to a new course of events was first paved by the introduction of minisatellites using multilocus probes (MLPs), providing incomparably higher discriminatory power. However, the procedures for multilocus typing were too arduous and required very large amounts of intact DNA which were usually not recovered from the difficult forensic biological specimens. This paved way for initiation of single locus probes (SLPs) in VNTR (variable number of tandem repeats) detection analysis. Analysis was stared with isolation of DNA from samples done by chloroform: isoamylalcohol method followed by the estimation of DNA in the samples with a standard DPA (method by comparing with standard commercial DNA. After that RFLP method was followed. The aim of our paper is identification of crime assailant with the aid of current DNA technology.Keywords
DNA Typing, RFLP, Forensic Investigation, VNTR, DPA, Minisatellites
References
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- Evaluation of Human Exposure to Vibrations using Quarter Car Model with Semi-Active Suspension
Abstract Views :334 |
PDF Views:156
Authors
D. V. A. Rama Sastry
1,
K. V. Ramana
2,
N. Mohan Rao
3,
M. Phani Kumar
2,
V. S. S. Rama Chandra Reddy
2
Affiliations
1 Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, IN
2 Dept. of Mech. Engg., Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, IN
3 Dept. of Mech. Engg., University College of Engg., JNTUK University, Kakinada, Andhra Pradesh, IN
1 Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, IN
2 Dept. of Mech. Engg., Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, IN
3 Dept. of Mech. Engg., University College of Engg., JNTUK University, Kakinada, Andhra Pradesh, IN
Source
International Journal of Vehicle Structures and Systems, Vol 10, No 4 (2018), Pagination: 268-272Abstract
Exposure of human body to vehicular vibrations in transit may lead to the human discomfort. Ride comfort is one of the major issues in design of automobiles. Magneto rheological (MR) dampers are emerging as most feasible solution for various applications in controlling vibrations. An MR damper is a semi-active device, which will offer the advantages of both active and passive suspension. In this study, the MR damper based semi-active suspension system for a car is analysed for ride comfort of 7 degrees of freedom model human body lumped mass, considering head, upper torso, lower torso and pelvis, seated over a seat of a quarter car model and is compared with that of similar system using passive damper. A MR damper is fabricated and is filled with MR fluid made of Carbonyl iron powder and Silicone oil added with additive. Modified Bouc-Wen Model developed by Spencer is used to model the behaviour of MR damper. All the parameters of this model are identified using data acquired from experiments conducted to characterise MR damper. Further, using the Spencer model of MR damper, the human body seated over quarter car is simulated by implementing a semi-active suspension system for analysing the resulting displacement and acceleration of the human body. The ride comfort performance of vehicle model with passive suspension system is compared with corresponding semi-active suspension system. The simulation and analysis are carried out using MATLAB/SIMULINK.Keywords
Magneto Rheological Dampers, Quarter Car Model, Suspension Systems, Spencer Model, Human Body Vibrations.Full Text
References
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- Investigation on the Correlation between Surface Roughness and Acoustic Emission Characteristics in Turning Process
Abstract Views :285 |
PDF Views:0
Authors
Affiliations
1 Surface Engineering and Tribology Laboratory, CSIR-Central Mechanical Engineering Research Institute, Durgapur, IN
2 Material Processing and Microsystem Laboratory, CSIR-Central Mechanical Engineering Research Institute, Durgapur, IN
1 Surface Engineering and Tribology Laboratory, CSIR-Central Mechanical Engineering Research Institute, Durgapur, IN
2 Material Processing and Microsystem Laboratory, CSIR-Central Mechanical Engineering Research Institute, Durgapur, IN
Source
Manufacturing Technology Today, Vol 18, No 6 (2019), Pagination: 3-9Abstract
One of the most significant feature for monitoring in machining processes is tool wear. It has a direct influence on the quality of machined surfaces. In-order to maintain the product quality and to reduce material wastage, online tool wear monitoring has become a regular practice. With the progress in tool wear, surface roughness changes accordingly and this change can be used to assess the tool condition. However, it is difficult to measure the surface roughness online. It is well known that interaction of tool and workpiece results in high frequency stress waves known as Acoustic Emissions (AE), which can be used as an indirect online method to monitor the surface roughness and in-turn tool wear. The analysis of AE signals which received significant attention in structural and machine health monitoring opens wide opportunities to monitor the machining process. Hence, in the present work an attempt has been made to explore the correlation between the acoustic emission characteristics and workpiece surface roughness during the high speed turning operation using AISI 4340 alloy steel workpiece with the help of Zirconia Toughened Alumina (ZTA) tool on a lathe machine. Experiments has been designed as per Central Composite Design (CCD) of Response Surface Methodology (RSM) with varying 3 levels of 3 parameters such as cutting speed, feed rate and depth of cut. For each experiment, AE signals are acquired and surface roughness is measured using Surtronic 25 portable surface roughness meter. Analysis of variance (ANOVA) is used to study the effect of control parameters on output responses and a model is prepared using regression analysis. It is observed from the ANOVA analysis that feed rate and cutting speed have profound influence on surface roughness and RMS respectively. The optimum condition is found at Cutting speed of 300 m/min with feed rate of 0.12 mm/rev and depth of cut of 1.5 mm with 97.15% desirability for minimum surface roughness and nominal RMS value. From the parametric study, it is observed that AE characteristic (RMS) shows good correlation with surface roughness which can be used for further analysis in online monitoring of tool wear.Keywords
AE Characteristics, RMS, Surface Roughness, Tool Wear.References
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