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Narasimha Rao, M.
- The Estimation of Nilotinib in Capsule Dosage Form by RP-HPLC
Authors
1 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy, Hyderabad-500 034, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 1, No 4 (2011), Pagination: 100-102Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Nilotinib in capsule dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotassium hydrogen orthophosphate in water pH-2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 266 nm. The retention time was 8.508 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 81268.215X+174323.3. The limit of detection and limit of quantification was 0.1μg and 0.3μg/ml respectively. The percentage assay of Nilotinib was 99.61%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Nilotinib in bulk drug and in its pharmaceutical dosage form.
Keywords
Nilotinib, RP-HPLC, Estimation, and Capsules.
- The Estimation of Raltigravir in Tablet Dosage Form by RP-HPLC
Authors
1 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, IN
2 Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam – 530003, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 1, No 3 (2011), Pagination: 56-58Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Raltigravir in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotasium hydrogen orthophosphate in water pH 2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 13.310 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y=56490.819X - 302507. The limit of detection and limit of quantification was 0.2μg and 0.6μg/ml respectively. The percentage assay of Raltigravir was 98.86%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Raltigravir in bulk drug and in its pharmaceutical dosage form.
Keywords
Raltigravir, RP-HPLC, Estimation, and Tablets.- The Estimation of Nilotinib in Capsule Dosage Form by RP-HPLC
Authors
1 Centre For Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam - 530003, IN
2 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy, Hyderabad-500 034, IN
Source
Asian Journal of Pharmacy and Technology, Vol 1, No 3 (2011), Pagination: 82-84Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Nilotinib in capsule dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotassium hydrogen orthophosphate in water pH-2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 266 nm. The retention time was 8.508 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 81268.215X+174323.3. The limit of detection and limit of quantification was 0.1μg and 0.3μg/ml respectively. The percentage assay of Nilotinib was 99.61%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Nilotinib in bulk drug and in its pharmaceutical dosage form.
Keywords
Nilotinib, RP-HPLC, Estimation, and Capsules.- The Estimation of Darunavir in Tablet Dosage form By RP-HPLC
Authors
1 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, IN
2 Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam - 530003, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 1, No 3 (2011), Pagination: 74-76Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Darunavir in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotassium hydrogen orthophosphate +0.02M Potassium Dihydrogen orthophosphatein in water and Acetonitrile in the ratio of 40:60 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 265 nm. The retention time was 5.859 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 44076.08X +726603. The limit of detection and limit of quantification was 0.1μg and 0.3μg/ml respectively. The percentage assay of Darunavir was 98.58%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Darunavir in bulk drug and in its pharmaceutical dosage form.
Keywords
Darunavir, RP-HPLC, Estimation, and Tablets.- The Estimation of Maraviroc in Tablet dosage form by RP-HPLC
Authors
1 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, IN
2 Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam- 530003, IN
3 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 3, No 5 (2011), Pagination: 230-232Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Maraviroc in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5m partical size, with mobile phase consisting of 0.02M Dipotasium hydrogen orthophosphate in water pH 2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 0.8 ml/min and the effluents were monitored at 210 nm. The retention time was 4.330 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 31018.059X + 44222.4. The limit of detection and limit of quantification was 0.2μg and 0.6μg/ml respectively. The percentage assay of Maraviroc was 99.42%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Maraviroc in bulk drug and in its pharmaceutical dosage form.
Keywords
Maraviroc, RP-HPLC, Estimation, and Tablets.- Enzymatic Synthesis of Ethyl Butyrate in Supercritical Carbon Dioxide Using Surface Coated Lipase from Candida rugosa
Authors
1 Department of Chemical Engineering, MVGR College of Engineering, Chintalavalasa-535005, Vizianagaram, Andhra Pradesh, IN
2 Pt. Ravishankar Shukla University, Raipur-492010 (CG), IN
3 Al Ameer College of Engineeering, Gudilova, Anandapuram, Bheemunipatnam-531173, Visakhapatnam, Andhra Pradesh, IN
Source
International Journal of Technology, Vol 1, No 2 (2011), Pagination: 72-75Abstract
The synthesis of low molecular weight ester, ethyl butyrate (used as a fruity flavor) by green chemistry is of immense importance to the food industry. Experiments were performed using Surface Coated Lipase (SCL) from Candida rugosa in supercritical carbon dioxide. The stability of enzyme in supercritical CO2 environment was validated and the influence of pressurization and depressurization on stability of enzyme was also studied. No significant loss of enzyme was noticed during pressurization and depressurization conditions even after 10 cycles. Further, experiments were performed to understand the effect of incubation time, temperature, enzyme concentration, reaction pressure on ester formation. The formation of ester gradually increased with incubation time. However, a steep increase in ester formation has been noticed between 7 and 10 h of incubation time. The reaction temperature appeared to have a profound effect on the product formation. The percent esterification increased linearly with increase in temperature and reached a maximum value of 92 % at 40°C and then decreased beyond this temperature. The percent esterification increased with the increase in enzyme concentration. The maximum percentage esterification of 92 % was observed at higher enzyme concentration [25 % w/w]. Reaction pressure seems to have marginal impact on the extent of alcoholysis and enzyme stability.Keywords
Alcoholysis, Surface Coated Lipase, Stability, Pressurization, Depressurization, Incubation Time.- The Estimation of Etravirine in Tablet dosage form by RP-HPLC
Authors
1 Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy, Hyderabad-500 034, IN
2 Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Visakhapatnam- 530003, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 10 (2011), Pagination: 1649-1651Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Etravirine in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5μm partical size, with mobile phase consisting of 0.03M Potassium Dihydrogen orthophosphate in water pH-3.2 with orthophosphoric acid and Acetonitrile in the ratio of 30:70 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 309 nm. The retention time was 9.111 min. The detector response was linear in the concentration of 80-240μg/ml. The respective linear regression equation being Y= 58030X + 86395.9 the limit of detection and limit of quantification was 0.1μg and 0.3μg/ml respectively. The percentage assay of Etravirine was 98.46%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Etravirine in bulk drug and in its pharmaceutical dosage form.