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Jattan, M.
- Genetic Divergence Studies in Plus Trees of Pongamia pinnata (Karanj)
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Indian Forester, Vol 135, No 8 (2009), Pagination: 1069-1080Abstract
Thirty plus trees of Pongamia pinnata (Karanj) were selected and their progeny was tested in the nursery. Substantial variability was recorded in seed morphological and biochemical traits of selected plus trees. Significant variation was observed in progenies for germination percentage, shoot and ischolar_main length, collar diameter and seedling biomass. Heritability and genetic advance as per cent of mean were also moderate to high for 100 seed weight, oil and protein contents, collar diameter, ischolar_main and shoot length indicating the effectiveness in selection for these characters for developing productive trees. Genetic diversity among these plus trees for important seed and seedling traits using Mahalanobis' D2 analysis showed grouping of these trees into eight clusters. The clustering pattern indicated that geographical diversity did not have one to one relationship with genetic diversity. Cluster VI was the largest and consisted of seven trees followed by Clusters I and IV with six trees each. Cluster III had only one tree. Intra cluster D2 values revealed that cluster VIII was most diverse (5.423) followed by cluster IV (4.131), V (4.081), I (3.915) and cluster II (3.831). Maximum inter-cluster distance was observed between clusters III and VIII (9.922) followed by between cluster V and VIII (7.085), cluster IV and VIII (6.738) and cluster III and V (6.722)). On the basis of high cluster mean and wide genetic distance, the superior plus trees of cluster III (PT- 19) and cluster V (PT- 8, PT-18. PT-24) may be used as potential parents for P. pinnata tree improvement programme.Keywords
Pongamia pinnata (Karanj), Genetic Divergence Studies, Plus Trees
- RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)
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Affiliations
1 CCS Haryana Agricultural University, Hisar-125004, IN
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, DE
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, IN
1 CCS Haryana Agricultural University, Hisar-125004, IN
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, DE
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, IN
Source
Indian Forester, Vol 139, No 12 (2013), Pagination: 1097-1104Abstract
Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.Keywords
Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPDReferences
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