Informatics Publishing Limited

M. K. Prasanna Kumar ¹, Soniya Agrawal ², Hemachandra Bhat ³

Affiliations
1 Department of Telecommunication, BMSCE, Bangalore, IN
2 Department of Electrical and Electronics, BMSCE, Bangalore, IN
3 Wipro Technologies, Bangalore, IN

Abstract

The objective of this paper is to present the effectiveness of Project Based Learning incorporating the development of student's soft skills like communication and team work as well as technical or professional competences such as deep thinking and learning. A case study in the form of one month value added course on Project based learning using Unified Technology Learning Platform (UTLP) for Electrical cluster students is implemented. The learning outcome of the student is evaluated at regular intervals during the course. These outcomes are driven by institution's vision of the knowledge and skills that students need to acquire in order to be independent and a life long learner. This paper shows how project based learning can be used to facilitate for the production of qualified and well prepared professionals. This paper also presents an approach for the project assessment used to make the learning outcomes effectively.

Keywords

Outcome Based Education (OBE), Unified Technology Learning Platform (UTLP), Project Based Learning (PBL), Indusry Institute Interaction.

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C. Mahadevaiah ¹, M. K. Prasanna Kumar ², Shailaja Hittalmani ¹

Affiliations
1 Marker Assisted Selection Laboratory, Department of Genetics and Plant Breeding, University of Agricultural Sciences, Bengaluru 560 065, IN
2 Department of Plant Pathology, University of Agricultural Sciences, Bengaluru 560 065, IN

Abstract

Accurate measurement of parameters contributing to diseases and their symptoms is important for identification of resistant genotypes. Present sheath rot scoring system such as disease incidence scoring system accounts for only percentage of disease incidence and not the disease severity. Disease severity scoring system is based on combined features of panicle exertion and lesion size, very laborious and less accurate in field condition. Existing disease index requires assigning of incidence-severity scores for each and every tillers in a plant and has greater difficulties in evaluation of large number of plants or breeding pools. Hence, we propose phenotypic parameters such as proportion of panicle exertion, proportion of diseased and healthy sheath and modified disease severity scoring system for accurate measurement of disease. A high-throughput and simple disease indices (severity index, lesion index, detached lesion index and panicle discolouration index) are proposed for accurate identification of resistant-susceptible genotypes.

Keywords

Dissecting Parameters, Oryza sativa, Sarocladium oryzae, Sheath Rot.

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C. R. Jahir Basha ¹, C. P. Manjula ¹, M. K. Prasanna Kumar ¹

Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN

Abstract

Study was undertaken to know the effective management strategy through novel bio control agents and botanicals against Ralstonia solanacearum a dreaded pathogen causing bacterial wilt of tomato. The study revealed that Bacillus subtilis was found to be most effective in inhibiting the growth of the pathogen followed by Pseudomonas fluorescens, by producing an inhibition zone of 22.50 mm and 18.00 mm in diameter, respectively under in vitro conditions. However, among plant extract tested Isabgol seed extract was found to be highly inhibitory to the growth of the pathogen in which inhibition of 22.00, 19.50 and 18.00 mm was observed at 1:0, 1:1 and 1:5 dilutions. Similarly under field condition, Bacteriophages and Bacillus subtilis were found to be very effective in reducing the disease incidence by 72.50 per cent and 64.58 per cent with reduced soil population of the pathogen both in soil and rhizosphere.

Keywords

Ralstonia solanacearum, Bacterial Antagonists, Botanicals.

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Rajeshwari Hegde ¹, M. K. Prasanna Kumar ¹, Gopalan Oppiliappan ¹

Affiliations
1 Dept. of TCE, BMSCE, Bangalore, IN

Abstract

Today, industry needs highly skilled engineering graduates to combat real world challenges in an efficient manner. The mere knowledge of theoretical concepts would not help the students to solve the global challenges. As such, nurturing young minds to appreciate complex problems and to think of innovative solutions either to alleviate prevalent problems or to improve the quality of life become the responsibility of technical institutions. The project contests to showcase the talents would help the students to exhibit their skills and project their ideas with peers and also with the industry experts. In this paper, the learning outcomes of one such national level project contest “TELECOMBAT”, held at the Department of Telecommunication Engineering, BMS College of Engineering, Bangalore has been presented. The contest aimed to bring together industry and academia experts to strengthen the industry-institution interaction and also to get the students' projects evaluated by the industry experts which would help the students to understand the need for implementing quality projects to excel in their professional career. The suggestions given by the experts have been incorporated for evaluating student's projects for the successive years. The rigorous evaluation of the projects involved three rounds with different rubrics for each round. This event provided a platform for institute and industry iteration and also helped students to get first hand feedback from experts and researchers.

Keywords

Learning Outcome, Project Contest, PBL, OBE.

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Priyanka Krishna ¹, M. K. Prasanna Kumar ¹, Manjunatha Channappa ², Pramesh Devanna ³, Kartar Singh ⁴, Puneeth M. Eeregowda ¹, H. B. Mahesh ¹, B. S. Chandrashekar ¹, Venkatesh Babu ⁵, Radhika U. Desai ¹, Sahana N. Banakar ¹

Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences (UAS), Bengaluru 560 065, IN
2 ICAR-Indian Agricultural Research Institute, Regional Station, Wellington 643 231, IN
3 Agricultural Research Station, Gangavati, UAS, Raichur 584 104, IN
4 ICAR-National Bureau of Plant Genetic Resources, Regional Station, Jodhpur 342 005, IN
5 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 005, IN

Abstract

Bacterial blight caused by Xanthomonas axonopodis pv. punicae (Xap) is one of the economically important diseases of pomegranate in India. Under field conditions, the disease is being managed using antibiotics and copper-based compounds but with limited success due to their poor bio-efficacy. The reduced efficacy of antibiotics and copper compounds against field populations of Xap might be due to the development of bactericide resistance through acquired genes. In the present study, ten bacterial blight-infected pomegranate samples were collected from different geographic locations of Karnataka, India, and causal agent Xap was isolated and identified through 16S rRNA sequencing. Streptomycin resistance genes such as rpsL, strA, strB and copper resistance genes copL, copB were detected using gene-specific primers in PCR. All ten isolates were positive for streptomycin resistance genes whereas copper resistance gene copB was absent in three isolates (Xap1, Xap4, Xap6) while copL was absent in Xap4 and Xap6 isolates. Further, in vitro experiments using different concentrations of streptomycin on culture media showed lowest growth inhibition up to 1500 μg/ml concentration, supporting the molecular evidence of antibiotic resistance. The present study provides preliminary information on the presence of antibiotic resistance genes in the field populations of Xap.

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S. RUQIYA ¹, H. C. GIRISHA ¹, C. MANJUNATHA ², R. RANGESHWARAN ², A. KANDAN ², G. SIVAKUMAR ², M. K. PRASANNA KUMAR ³, D. PRAMESH ⁴, K. T. SHIVAKUMARA ², H. S. VENU ², S. NANDITHA ², K. S. ANKITHA ¹, K. ADITYA ², N. AARTHI ², S. N. SUSHIL ²

Affiliations
1 Department of Agricultural Microbiology, UAS, GKVK, Bengaluru – 560 065, Karnataka, IN
2 ICAR-National Bureau of Agricultural Insect Resources, Bengaluru – 560024, Karnataka, IN
3 Department of Plant Pathology, UAS, GKVK, Bengaluru – 560065, Karnataka, IN
4 Rice Pathology Laboratory ARS, Gangavathi, UAS Raichur – 584104, Karnataka, IN

Abstract

Bacillus subtilis  is  a Gram-positive and endospore producing bacterium. Limited studies have shown that lipopeptides produced by  B. subtilis  can be inhibitory to phytopathogens.   Sclerotinia sclerotiorum  is a plant pathogenic fungus  which causes  various diseases like cotton rot, watery soft rot, stem rot, crown rot and blossom blight in vegetable crops. The objective of the study was to isolate lipopeptides from  B. subtilis  and study their inhibitory potential against  S. sclerotiorum. So, the B. subtilis  isolates  were extracted from the collected soils of Western Ghats of India. They were initially characterized through morphological parameters followed by PCR amplification of the 16S rDNA gene and confirmation through BLAST algorithm in NCBI database. The lipopeptides produced by these isolates were tested against  S. sclerotiorum. B. subtilis  strains were effective against  S. sclerotiorum and exhibited 18.33 to 29.5 % inhibition under dual culture bio-assay. The antagonistic activity of lipopeptides extracted from  B. subtilis  strains showed 21.56 to 88.89 % inhibition of  S. sclerotiorum  in the lowest to highest concentration of lipopeptide tested and was found to be significantly higher than the control. The present study has shown that  B. subtilis  strains vary in the production of lipopeptides and some of them could produce lipopeptides that are highly inhibitory to S. sclerotiorum. B. subtilis  strain NBAIR BSWG1 showed the highest inhibition for S. sclerotiorum. Lipopeptide based poison food technique and the dual culture bioassay results showed that B. subtilis  strain NBAIR BSWG1 has immense potential for use in the biological control of  S. sclerotiorum. Further studies are being carried out in formulating the lipopeptides for field application.

Keywords

Antimicrobial property, biopesticide, PCR, soft rot of vegetable, Western Ghats.

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