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Authors
Affiliations
1 Institute of Porous Flow and Fluid Mechanics, Chinese Academy of Science, Langfang, Hebei 065007, CN
2 Research Institute of Petroleum Exploration and Development (Langfang), Langfang, Hebei 065007, CN
3 EADBIO Center, Elche, 06079, Alicante, ES
Source
Nature Environment and Pollution Technology, Vol 13, No 2 (2014), Pagination: 321-326
Abstract
This paper examines the total bacterial concentration and the functional bacteria concentration of the injection water from the injection well LU3064 and the produced fluid from the production well LU3036 using the plate count method and the most-probable-number method. We find that the bacterial community of the injection water is more complex and the bacteria concentration is higher than those of the produce fluid. Additionally, the surface tension of the produced fluid is lower than that of the injection water by 12%. We performed a diversity analysis to the injection water from LU3064 and the produced fluid from LU3036 by constructing the 16S rDNA clone library using molecular biology approaches. Through the comparison to GenBank, we found that the community in the injection water has a high diversity. The dominant bacteria belong to Alphaproteobacteria. The bacterial community of the produced fluid is relatively simpler, where Pseudomonas constitutes more than 80%, which are the dominant bacteria. There are also other functional bacteria such as Bacillus that exists in the produced fluid. We conclude that the oil recovery of Luliang oil field can be increased by microorganism flooding method.
Keywords
Luliang Oil Field, Microbial Community, 16S rDNA Library, Injection Well, Oil Well.
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