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Burela, Laxminarayana
- Trends in Wheat Germ Cell Free Protein Expression System with an Emphasis on Up-scaling and Industrial Application
Abstract Views :371 |
PDF Views:160
Authors
K. Bhanu Revathi
1,
V. A. Ramaiah
2,
Alan G. Hinnebusch
3,
Abani K. Bhuyan
4,
K. S. R. Sivasai
1,
Akulapalli Sudhakar
5,
Laxminarayana Burela
1
Affiliations
1 Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, IN
2 Dept. of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, IN
3 Laboratory of Gene Regulation and Development, National Instt. of Child Health and Human Development, National Instt. of Health, Bethesda, MD 20892, IN
4 School of Chemistry, University of Hyderabad, Hyderabad, IN
5 Cell Signaling and Tumor Angiogenesis Lab, Dept. of Genetics, Boys Town National Research Hospital, Omaha, NE, US
1 Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, IN
2 Dept. of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, IN
3 Laboratory of Gene Regulation and Development, National Instt. of Child Health and Human Development, National Instt. of Health, Bethesda, MD 20892, IN
4 School of Chemistry, University of Hyderabad, Hyderabad, IN
5 Cell Signaling and Tumor Angiogenesis Lab, Dept. of Genetics, Boys Town National Research Hospital, Omaha, NE, US
Source
Indian Journal of Science and Technology, Vol 3, No 3 (2010), Pagination: 349-354Abstract
Cell-free translation systems have developed significantly over the last 2 decades and improvements in yield have resulted in their use for in vitro protein production in the laboratory. Such cell free systems enable the expression of cytotoxic, regulatory or unstable proteins that cannot be expressed in living cells. We compare the uses of different cell-free translation systems and review recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression.Keywords
Cell Free Biology, in vitro Protein Expression, Scale up of Translation, Wheat Germ ExtractFull Text
References
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