Refine your search
Co-Authors
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Singh, Kuldip
- Alterations in Glutathione Levels as a Marker of Oxidative Stress in Smokers
Abstract Views :454 |
PDF Views:0
Authors
Affiliations
1 Department of Biochemistry, Government Medical College Campus, Amritsar Punjab, IN
1 Department of Biochemistry, Government Medical College Campus, Amritsar Punjab, IN
Source
International Journal of Medical Sciences, Vol 6, No 1 (2013), Pagination: 1-5Abstract
Background : Cigarette smoking has been implicated as a significant risk factor for the initiation and establishment of several diseases like coronary artery disease and associated with high incidences of morbidity and mortality.
Aim: To assess total-sulfhydryl, protein bound-sulfhydryl and non-protein bound-sulfhydryl group levels and glutathione metabolizing enzyme such as glutathione reductase activity in serum of smokers and coronary artery disease patients in comparison with that of healthy non-smokers and to ascertain whether chronic smoking is an initiator of oxidative stress or not.
Material and Methods: 75 subjects were recruited in the present study which were divided into three groups; in group-1; 25 known coronary artery disease patients, in group-2: 25 subjects of smokers were recruited and in the group-3: 25 normal healthy non smokers were recruited from the north Indian population of Punjab. Fasting blood samples were taken from all the subjects of three groups for the determination of total-sulfhydryl, protein boundsulfhydryl and non-protein bound-sulfhydryl levels and glutathione reductase activity.
Results : A significant (p=0.001) reduction in total-sulfhydryl, protein bound-sulfhydryl and non-protein boundsulfhydryl group levels and glutathione reductase activity was observed in coronary artery disease patients (Group- 1) and smokers (Group-2) in comparison to normal healthy non-smokers (Group-3). A statistical no significant decrease was recorded in glutathione levels and glutathione reductase activity in smokers (Group-2) as compared to coronary artery disease patients (Group-1).
Conclusion: These observations suggested that smoking induced the oxidative stress by reducing the glutathione levels and glutathione reductase activity, might be responsible for the pathophysiology of coronary artery diseases in the younger generation.
Keywords
Coronary Artery Disease (cAD), Reduced Glutathione (GSH), Oxidative Stress, Smoking
- Physiological and Pathological Role of Reactive Oxygen Species
Abstract Views :285 |
PDF Views:0
Authors
Affiliations
1 Department of Biochemistry, Government Medical College Campus, Amritsar Punjab, IN
2 Krishi Vigyan kendra, Shamsher Nagar sirhind, Fatehgarh Sahib Punjab, IN
1 Department of Biochemistry, Government Medical College Campus, Amritsar Punjab, IN
2 Krishi Vigyan kendra, Shamsher Nagar sirhind, Fatehgarh Sahib Punjab, IN
Source
International Journal of Medical Sciences, Vol 6, No 1 (2013), Pagination: 31-48Abstract
Reactive oxygen species occur continuously in all the cells as a part of normal cellular function. These reactive oxygen species are a potential double -edged sword in diseases prevention and promotion. The most common reactive oxygen species are hydrogen peroxide, superoxide ion and hydroxide radical are the compounds, when present in a high enough concentration, can damage cellular proteins, lipids and nucleic acids that may promote various diseases. Advances in research have shown that low to moderate concentrations of reactive oxygen species play crucial roles in normal physiological processes, such as through redox regulation of protein phosphorylation, ion channels and transcription factors etc. and are also required for biosynthetic processes, including thyroid hormone production and cross linking of extracellular matrix. Reactive oxygen species induced disease, can be either due to a lack of reactive oxygen species (e.g., chronic granulomatous disease, certain autoimmune disorders) or a surplus of reactive oxygen species (e.g., cardio-vascular and neurodegenerative diseases). The human body has several mechanisms to counteract the reactive oxygen species by producing antioxidants, which are either naturally produced in situ or externally supplied through foods and /or supplements. Antioxidant supplementation has proven largely ineffective in clinical studies, most probably because their action is too late, too little and too nonspecific. This article reviews the basic chemistry, mechanisms of formation and catabolism of reactive oxygen species, their beneficial effects and the consequences of free radical induced diseases in the body.Keywords
Reactive Oxygen Species (ROS), Free Radicals, Antioxidants, Cancer, Cardio-vascular Disease (CVD)- Clinical Trial of Diphenyl Pyraline ('Histryl') in Atopic Rhinitis
Abstract Views :184 |
PDF Views:0
Authors
Affiliations
1 Medical College, Amritsar, IN
1 Medical College, Amritsar, IN
Source
The Indian Practitioner, Vol 30, No 2 (1977), Pagination: 67-70Abstract
No AbstractKeywords
No Keywords- Impact on Some Antioxidant Enzymes and Vitamins in Obese Adolescents of North West Indians
Abstract Views :424 |
PDF Views:0
Authors
Kuldip Singh
1,
Sukhjit Kaur
2
Affiliations
1 Department of Biochemistry, Government Medical College, Patiala-147001, Punjab, IN
2 Department of Biochemistry, Government Medical College, Amritsar-143001, Punjab, IN
1 Department of Biochemistry, Government Medical College, Patiala-147001, Punjab, IN
2 Department of Biochemistry, Government Medical College, Amritsar-143001, Punjab, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 2 (2015), Pagination: 97-102Abstract
Obesity is one of the most common health problems in developed and developing nations such as India. In India, prevalence of obesity among adolescents is 6-24 %. In addition, obesity is considered as one of the risk factors for the initiation of many chronic diseases. Oxidative Stress (OS) is a well-established hallmark of diseases such as diabetes mellitus, cancer, and cardiovascular diseases. The aim of the study was to evaluate OS markers in obese subjects with respect to normal healthy subjects. The present case-control study was carried out on 25 male obese adolescent children with body mass index (BMI) ≥25 kg/m2 and on equal number of male normal healthy children with BMI<15 kg/m2 in the age range of 10-19 years. These subjects were recruited from rural and urban areas of Amritsar District, Punjab, India. The levels of malondialdehyde (MDA), antioxidant enzymes/molecules such as superoxide dismutase (SOD), reduced glutathione (GSH), Glutathione Reductase (GR), Glutathione Peroxidase (GPx), antioxidant Vitamin E and Vitamin C, and β-carotene along with total antioxidant activity (TAA) were evaluated. Significant increase in the concentration of MDA by 114.81% was observed in obese adolescents in comparison to normal healthy adolescents while a significant fall was observed in SOD (47.153%), GSH (60.79%), GR (43.39%), GPx (29.92%), TAA (54.08%), Vitamin E (45.45%), Vitamin C (56.14%), and β-carotene (32.88%) levels in obese adolescents as compared to normal healthy control adolescents. Aforementioned observations suggested that OS induced in the obese adolescents even in the absence of any chronic diseases. Therefore, obesity should be treated at the early stages to prevent further complications.Keywords
Antioxidants, Cardiovascular Disease, Malondialdehyde, Obesity, Oxidative Stress, Reduced Glutathione Superoxide Dismutase, Vitamin E, Vitamin C.References
- Alok P, Malay P, Divyeshkumar V. Prevalence of overweight and obesity in adolescents of urban and rural area of Surat, Gujarat. Natl Med Res. 2012; 2:325–9.
- Banjade B, Naik VA, Narasannavar A. Prevalence of obesity and its risk factors among pre-university college adolescents of Belgaum city, Karnataka. J DentMedSci. 2014; 13:56–60.
- Raj M, Kumar RK. Obesity in children & adolescents. Indian J Med Res. 2010; 132:598–607.
- Kaur J, Mehta P. A study of prevalence of overweight and underweight among girls from different socioeconomic status in Ludhiana (Punjab). Hum Biol Rev. 2012; 1:197–206.
- Sidhu S, Gauri M, Kaur P. Prevalence of overweight and obesity among the affluent adolescent school children of Amritsar, Punjab. Coll Antropol. 2005; 29:53–5.
- Singh K. Studies on the effect of monosodium glutamate [MSG] on oxidative stress in certain tissues of adult mice. 1sted. India: Statperson Publication Corporation. 2015.
- Singh K. Role of Reactive Oxygen Species in Health and Diseases. Germany: Lap Lambert Academic Publishing; 2014; 1–60.
- Singh K, Ahluwalia P. Effect of oral ingestion of monosodium glutamate administration on various lipid fractions and certain antioxidant enzymes in cardiac tissue of chronic alcoholic adult male rats. J Cardiovas Dis Res. 2012; 3:215–21.
- Kuldip S, Bal BS, Seema C, Saranpal S, Naresh M. Ameliorative effect of lycopene on lipid peroxidation and certain antioxidant enzymes in diabetic patients. J Diabetes Metab. 2012; 3:6–10.
- Sukhjit K, Kuldip S. Comparative study on MDA and SOD in IHD patients and healthy smokers. Germany: Lap Lambert Academic Publishing; 2014; 1–210.
- Brieger K, Schiavone S, Miller FJ Jr, Krause KH. Reactive oxygen species: From health to disease. Swiss Med Wkly. 2012; 142:13659.
- Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, et al. Oxidative stress, prooxidants, and antioxidants: THe interplay. Biomed Res Int. 2014; 2014:761264.
- Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. ClinChimActa. 1978; 90:37–43.
- Jaiswal D, Rai PK, Mehta S, Chatterji S, Shukla S, Rai DK, et al. Role of Moringaoleifera in regulation of diabetes-induced oxidative stress. Asian Pac J Trop Med. 2013; 6:426– 32.
- Giustarini D, Dalle-Donne I, Milzani A, Fanti P, Rossi R. Analysis of GSH and GSSG after derivatization with N-ethylmaleimide. Nat Protoc. 2013; 8:1660–9.
- Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70:158–69.
- Worthington DJ, Rosemeyer MA. Glutathione reductase from human erythrocytes. Molecular weight, subunit composition and aggregation properties. Eur J Biochem. 1975; 60:459–66.
- Baker H, Frank O. Clinical Vitaminology, Methods and Interpretations. New York: Interscience Publishers, John Wiley and Sons Inc. 1968; 172 pp.
- Quaife ML. Determination of serum ß carotene. In: Varley H, Gowenlock AH, McMurry, JR, McLauchlan DM editors. Varley’s Practical Clinical Biochemistry. 6th ed. London: CBS (New Delhi-India); 1968; 903 pp.
- Natelson S. Techniques in Clinical Biochemistry. Vol. 162. USA:CC Thomas. 1971; 288 pp.
- Benzie IF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a measure of antioxidant power: THe FRAP assay. Anal Biochem. 1996; 239:70–6.
- Marklund SL. Properties of extracellular superoxide dismutase from human lung. Biochem J. 1984; 220:269–72.
- Kim BJ, Hood BL, Aragon RA, Hardwick JP, Conrads TP, Veenstra TD, et al. Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells. Proteomics. 2006; 6:1250–60.
- Onyema OO, Farombi EO, Emerole GO, Ukoha AI, Onyeze GO. Effect of Vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats. Indian J Biochem Biophys. 2006; 43:20–4.
- Padmini E, Sundari BT. Erythrocyte glutathione depletion impairs resistance to haemolysis in women consuming alcohol. J ClinBiochem Nutr. 2008; 42:14–20.
- Choudhary P, Malik VB, Puri S, Ahluwalia P. Studies on the effects of monosodium glutamate on hepatic microsomal lipid peroxidation, calcium, ascorbic acid and glutathione and its dependent enzymes in adult male mice. Toxicol Lett. 1996; 89:71–6.
- Nakazawa H, Genka C, Fujishima M. Pathological aspects of active oxygens/free radicals. Jpn J Physiol. 1996; 46:15– 32.
- Wood LG, Garg ML, Smart JM, Scott HA, Barker D, Gibson PG. Manipulating antioxidant intake in asthma: A randomized controlled trial. Am J ClinNutr. 2012; 96:534–43.
- A Review on Power Management and Power Quality for Islanded PV Microgrid in Smart Village
Abstract Views :206 |
PDF Views:0
Authors
Affiliations
1 Centurion University of Technology and Management (CUTM), Rajaseetapuram, Odisha − 761211, IN
2 Guru Nanak Institute of Technology (GNIT), Hyderabad – 501506, Telangana, IN
1 Centurion University of Technology and Management (CUTM), Rajaseetapuram, Odisha − 761211, IN
2 Guru Nanak Institute of Technology (GNIT), Hyderabad – 501506, Telangana, IN
Source
Indian Journal of Science and Technology, Vol 10, No 17 (2017), Pagination:Abstract
Objective: To review the challenges of Power management and Power quality for islanded Photo-Voltaic (PV) microgrid for smart village electrification. Method: From different reviews, we understand the challenges and facts of power management and power quality for islanded PV Microgrid. The power management solutions are proposed based on smart management system w.r.t load demand and for improving the power quality intelligent Flexible AC Transmission Systems (FACTS) devices are proposed. Findings: The islanded PV microgrid a transformative solution to meet the energy demands for smart village or smart city. The intelligent control for power management and power quality will improve the reliability of islanded PV Microgrid in smart village. Applications: The smart village or smart city concept bring up by government of India for implement the modern services and modern electrification of rural area, which is the control pillar of Indian development.Keywords
Electrification, Intelligent, Power Management, Power Quality, PV Microgrid, Smart Village.Full Text
- Status of Plasma Malondialdeyde and Certain Antioxidant Enzymes During Normal Pregnancy in North Indian Population (Punjab)
Abstract Views :180 |
PDF Views:0
Objective: The objective of the present work was to investigate the alterations in oxidative stress markers by measuring malondialdehyde and certain antioxidant enzymes like xanthine oxidase, superoxide dismutase, glutathione, glutathione peroxidase and glutathione reductase in different trimesters.
Design of study: The level of malondialdehyde and antioxidant initiating enzyme xanthine oxidase and antioxidant scavenging enzymes like superoxide dismutase, glutathione, glutathione peroxidase and glutathione reductase was estimated in 200 healthy non pregnant and pregnant females ranging in age from 25 to 35 years.
Results: The level of malondialdehyde, xanthine oxidase and superoxide dismutase was significantly increased while the levels of glutathione, glutathione peroxidase and glutathione reductase were significantly decreased significantly in plasma of pregnant females with respect to non-pregnant females.
Conclusion: Our observations are suggesting that normal pregnant women's in the north Indian populations (Punjab) might be associated with an increase in oxidative stress.
Authors
Affiliations
1 Department of Biochemistry, Govt. Medical College, Amritsar (Punjab), IN
2 Department of Obstetrics and Gynecology, Guru Teg Bahadhur Hospital, Govt. Medical College, Amritsar (Punjab), IN
3 Department of Biochemistry, Punjab University, Chandigarh (Punjab), IN
1 Department of Biochemistry, Govt. Medical College, Amritsar (Punjab), IN
2 Department of Obstetrics and Gynecology, Guru Teg Bahadhur Hospital, Govt. Medical College, Amritsar (Punjab), IN
3 Department of Biochemistry, Punjab University, Chandigarh (Punjab), IN
Source
International Journal of Medical Sciences, Vol 3, No 1-2 (2010), Pagination: 14-18Abstract
Background: Free radicals and peroxides are involved in physiological phenomena such as synthesis of prostaglandin and thromboxanes and are thought to participate in pregnancy. Free radicals are also responsible for the pathogenesis of various diseases like atherosclerosis, inflammatory diseases and malignancies etc.Objective: The objective of the present work was to investigate the alterations in oxidative stress markers by measuring malondialdehyde and certain antioxidant enzymes like xanthine oxidase, superoxide dismutase, glutathione, glutathione peroxidase and glutathione reductase in different trimesters.
Design of study: The level of malondialdehyde and antioxidant initiating enzyme xanthine oxidase and antioxidant scavenging enzymes like superoxide dismutase, glutathione, glutathione peroxidase and glutathione reductase was estimated in 200 healthy non pregnant and pregnant females ranging in age from 25 to 35 years.
Results: The level of malondialdehyde, xanthine oxidase and superoxide dismutase was significantly increased while the levels of glutathione, glutathione peroxidase and glutathione reductase were significantly decreased significantly in plasma of pregnant females with respect to non-pregnant females.
Conclusion: Our observations are suggesting that normal pregnant women's in the north Indian populations (Punjab) might be associated with an increase in oxidative stress.
Keywords
Xanthine Oxidase (XOD), Lipid Peroxidation (LPO), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx).- Alteration in Glutathione and Its Metabolizing Enzymes in Cardiac Tissue Upon Oral Ingestion of Monosodium Glutamate to Hypercholestremic Adult Male Mice
Abstract Views :262 |
PDF Views:0
Authors
Affiliations
1 Department of Biochemistry, Govt. Medical College, Amritsar (Punjab), IN
2 Department of Horticulture, Punjab Agricultural University, Ludhiana (Punjab), IN
3 Department of Biochemistry, Punjab University, Chandigarh (U.T.), IN
1 Department of Biochemistry, Govt. Medical College, Amritsar (Punjab), IN
2 Department of Horticulture, Punjab Agricultural University, Ludhiana (Punjab), IN
3 Department of Biochemistry, Punjab University, Chandigarh (U.T.), IN
Source
International Journal of Medical Sciences, Vol 5, No 1-2 (2012), Pagination: 10-14Abstract
Monosodium glutamate (MSG) was orally administered at dose levels of 4 and 8mg/g body weight to hypercholestremic adult male mice for 6 consecutive days and its effect was observed on 31st day after the last injection by evaluating the changes in total-sulfhydryl (TSH) group, Non-protein bound sulfhydryl (NPB-SH) group and protein bound sulfhydryl (PB-SH) and glutathione metabolising enzymes like glutathione reductase (GR) and glutathione peroxidase (GPx) enzymes in cardiac tissue of hypercholestremic adult male mice. The animals were divided in four groups each comprising 6 mice. Group-I: Control, Group-II: Hypercholestremic animals, Group-III: 4mgMSG/g body weight + hypercholestremic animals and Group-IV: 8mgMSG/g body weight + hypercholestremic animals. Animals were fasted overnight and sacrificed by decapitation. The 10 per cent homogenate was prepared in 100mM potassium phosphate buffer (pH7.5). The homogenate was centrifuged at 1,000g and supernatant was used for the estimation of TSH, NPB-SH, PB-SH, GR and GPx. The levels of TSH, PBSH and NPBSH groups were significantly decreased in cardiac tissue of all the study groups. The glutathione metabolising enzymes such as GR and GPx was significantly decreased in cardiac tissue of hypercholestremic adult male mice's without MSG (Group-2) and with MSG (Group-3 and group-4) ingestion. These observations suggested that ingestion of MSG at dose levels of 4 and 8mg/g body weight to hypercholestremic animals had no beneficial effect instead it further weakens the antioxidant status in the cardiac tissue by significantly lower the levels of glutathione and its metabolising enzymes and thereby being responsible for the initiation of coronary heart disease/atherosclerosis.
Keywords
Monosodium Glutamate, Hypercholestremia, Coronary Heart Disease, Glutathione.- Comparative Growth Rate of Agriculture in Indian and Pakistani Punjab
Abstract Views :177 |
PDF Views:0
Authors
Kuldip Singh
1,
T. S. Chahal
2
Affiliations
1 Department of Forest and Wildlife, Ludhiana (Punjab), IN
2 Institute of Development and Planning, Amritsar (Punjab), IN
1 Department of Forest and Wildlife, Ludhiana (Punjab), IN
2 Institute of Development and Planning, Amritsar (Punjab), IN
Source
Agriculture Update, Vol 5, No 1-2 (2010), Pagination: 172-176Abstract
An attempt has been made in this paper to analyze the variation in performance of agricultural sector in post independence period in Indian and Pakistani Punjab. The area under wheat, cotton, sugarcane and maize is higher in Pakistani Punjab as compared to Indian Punjab but under rice it is lower in Pakistani Punjab as compared to Indian Punjab. In case of production of wheat, cotton and maize, it is higher in Pakistani Punjab as compared to Indian Punjab but it is lower in the case of rice and sugarcane. In case of yield of wheat, rice and maize it is higher in Indian Punjab as compared to Pakistani Punjab but it is lower in sugarcane and cotton in Indian Punjab during the period of 2003-04. This primarily happened due to adoption of various technologies at a faster rate in Indian Punjab as compared to its counter part in Pakistani Punjab.Keywords
Indian Punjab, Pakistani Punjab, Growth Rate, Production.- Effect of Monosodium Glutamate on Various Lipid Fractions and Certain Antioxidant Enzymes in Arterial Tissue of Chronic Alcoholic Adult Male Mice
Abstract Views :178 |
PDF Views:0