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Authors
Affiliations
1 Division of Genetics and Molecular Biology,
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MY
Source
Current Science, Vol 109, No 9 (2015), Pagination: 1709-1714
Abstract
Quorum sensing (QS) is a method of bacterial cell-tocell communication to coordinate adaptive behaviour. N-Acyl homoserine lactone (AHL) is the most studied signalling molecule among proteobacteria. In the present study, we characterize QS activity in Pantoea sp. A4, a new Rafflesia-associated bacterium. Results show the production of purple violacein by reporter bioassay Chromobacterium violaceum CV026 and bioluminescence activation in Escherichia coli [pSB401], indicating AHL production. Using liquid chromatography- mass spectrometry, Pantoea sp. was found to excrete four types of AHL, namely N-hexanoylhomoserine lactone, 3-oxo-N-hexanoylhomoserine lactone, N-octanoylhomoserine lactone and N-dodecanoylhomoserine lactone. To our knowledge, there is no previous documentation of Rafflesia-associated bacterium that produces long chain AHL.
Keywords
N-Acyl-Homoserine Lactone, Pantoea Sp., Quorum Sensing, Rafflesia.
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