Informatics Publishing Limited

Jie Deng ¹, Hongwei Rong ¹, Chaosheng Zhang ¹, Kefang Zhang ¹

Affiliations
1 Guangzhou University, Key Laboratory for Water Quality Security and Protection in Pearl River Delta, Ministry of Education and Guangdong Province, Guangzhou 510006, CN

Abstract

This paper is aimed to solve the effect of pH value in denitrification and phosphorus removal process, and to determine the best pH value for the treatment. pH value was an important indicator of activated sludge reaction in the water treatment process, and it was necessary to keep the suitable pH value to obtain a good treatment effect. The influence of anaerobic phosphorus release and anoxic phosphorus absorption under pH = 6.0, pH = 7.0 and pH = 8.0 in sequencing batch reactor was studied in this paper. The experiment results showed that, in anaerobic phase, anaerobic phosphorus release increased with the increase of pH value, and no significant change was observed in HRT for anaerobic. In anoxia phase, when pH = 6.0, the activity of denitrifying phosphorus removal bacteria was significantly inhibited and phosphorus absorption was poor. When pH = 7.0 and 8.0, the anoxic phosphorus uptake and denitrification system worked well. In the late stages of anoxic phase pH>8.0, the release and absorption of phosphorus was also very stable.

Keywords

Denitrification Phosphorus, Removal Sequencing Batch Reactor, pH Value, PAOs.

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Guanhua Gao ¹, Hongwei Rong ¹, Chaosheng Zhang ¹, Kefang Zhang ¹, Peilan Zhang ¹

Affiliations
1 Key Laboratory for Water Quality Security and Protection in Pearl River Delta, Ministry of Education and Guangdong Province, Guangzhou University, Guangzhou 510006, CN

Abstract

Through anaerobic culture test, from the six different anaerobic phosphorus removal sludge, it was found that Anaerobic Sequencing Batch Reactor (ASBR) sludge is the most appropriate sludge source to remove phosphorus in the liquid medium, followed by Expanded Granular Sludge Bed (EGSB) sludge and chicken manure. Microbial community structures in the six different sludge sources were investigated by 16S Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) method when the anaerobic systems operated steadily. The DGGE fingerprints were analysed by the software Quantity One to obtain the information of the microbial species in the six different sludge sources, which showed that there was a high diversity in the bacterial communities, and the richness value of ASBR sludge was 0.59 whose number occupied more than half of the total bands, while The richness value reached highest (0.61) when it was chicken manure. The community similarity between ASBR sludge and EGSB sludge is the highest 71.7. In the bands strength schematic diagram, the No. 1, No. 8 and No. 18 band that existed in ASBR sludge, EGSB sludge and chicken manure might represent the colony related to the anaerobic phosphorus microbe.

Keywords

Culture Test, Molecular Techniques, Anaerobic Phosphorus Sludge, PCR-DGGE, Microbial Community.

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