Informatics Publishing Limited

Hitesh V. Jasani ¹, Nimita G. Umretiya ², Manthan N. Kapuria ³, Darshan T. Dharajiya ¹, Tarun J. Khatrani ¹, Nalin K. Pagi ¹, Karen P. Pachchigar ¹, L. D. Parmar ¹

Affiliations
1 Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, IN
2 Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, IN
3 Shree P. M. Patel Institute of Integrated M. Sc. in Biotechnology, Anand 388001, Gujarat, IN

Abstract

Objectives: A protocol was developed for plant regeneration using cotyledonary node of high-yielding indigenous pigeonpea [Cajanus cajan (L.) Millsp.] genotype GT 101. Methods: Induction of multiple shoots directly was completed from cotyledonary node as an explants on Murashige and Skoog’s (MS) medium supplemented with various concentrations and combination of 6-benzylaminopurine (BAP), kinetin and α-naphthalene acetic acid (NAA). Elongation of multiplied shoots was performed on MS medium supplemented with different combination of BAP and GA3. These well elongated plantlets were further transferred on MS medium supplemented with various concentrations of indole-3-butyric acid (IBA) for ischolar_main induction. Regenerated plants were transferred to cocopeat:soil:vermiculite (2:1:2) for acclimation. Findings: The frequency of multiplication and number of multiple shoots from cotyledonary node explant were influenced on various types and concentrations of cytokinin. For multiple shoots induction, 3.0 mg/L BAP with 0.5 mg/L NAA was superior as compared to other combinations. The elongation of multiplied shoots was carried out on MS medium supplemented with 0.5 mg/L BAP and 0.5 mg/L GA3. The developed shoots were advanced to ischolar_maining on the medium supplemented with 0.5 mg/L IBA. They were subsequently grown in pots with 80% survival rate and these plants produced viable seeds. Improvement: The protocol for the production of in vitro multiple shoots with high frequency and their successive conversion to whole plants agreements potential for use in the improvement of protocol for development of transgenic in pigeonpea.

Keywords

Cajanus cajan (L.), Cotyledonary Node, GT101, In vitro regeneration, Pigeonpea.

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