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Dashora, Kamlesh
- Estimation of Rutin in Tephrosia purpurea by HPTLC Method
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Authors
Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
Source
Research Journal of Pharmacy and Technology, Vol 6, No 1 (2013), Pagination: 58-60Abstract
Tephrosia purpurea Linn (Family-Fabeaceae) having anti-hepatotoxic activity, CNS depressant activity and it also show anti-allergic activity. The plant Tephrosia purpurea contains rutin, quercetin, rotenoids, rotenone, tephrosin and lupeol. The powdered aerial part of Tephrosia purpuria was extracted with 95% ethanol by hot continuous extraction method. The prepared extracts were subjected to HPTLC analysis. The marker compounds were estimated by using pre-coated HPTLC aluminium silica gel 60 F254 plates (MERCK) and compared with standard. The methanolic solution of standard rutin 5 μl (1 mg/ml) and Tephrosia purpuria extract 8 μl (10 mg/ml) were applied as 7mm band width using CAMAG Linomat IV applicator. The Mobile phase is ethyl acetate: formic acid: glacial acetic acid: water (100:11:11:26). The detection was carried out at 366 nm. The amount of rutin was estimated by the comparing the peak area of standard and the same was present in the extracts. The content of rutin in Tephrosia purpurea extracts was found to be 1.40% w/w. This estimation technique is very much useful for the estimation of quercetin and rutin present in the various medicinal plants.Keywords
Rutin, HPTLC, Tephrosia purpuria
References
- V.U. Ahmad, Z. Ali, S.R. Hussaini, F. Iqbal, M. Zahid, M. Abbas, N. Saba, Flavonoids of Tephrosia purpurea. Fitoterapia. 70; 1999: 443-445.
- E Venkata Rao and N Ranga Raju, Two Flavonoids From Tephrosia purpurea. Phyrochemmy. 23(10); 1984: 2339-2342.
- A.S. Damre, A.B. Gokhale, A.S. Phadke, K.R. Kulkarni, M.N. Saraf, Studies on the mmunomodulatory activity of flavonoidal fraction of Tephrosia purpurea. Fitoterapia. 74; 2003: 257–261.
- Shenoy Smita, Shwetha K, Prabhu K, Maradi R, Bairy KL, Shanbhag T., Evaluation of anti inflammatory activity of Tephrosia purpurea in rats. Asian Pacific Journal of Tropical Medicine. 2010: 193-195.
- Ali Rifat Gulpinar, Ilkay Erdogan Orhan, Asuman Kan, Fatma Sezer Senol, Sadiye Ayse Celik, Murat Kartal, Estimation of in vitro neuroprotective properties and quantification of rutin and fatty acids in buckwheat (Fagopyrum esculentum Moench) cultivated in Turkey. Food Research International. 46; 2012: 536–543.
- Seth P. D. HPTLC. First edition, CBS Publishers and distributors, New Delhi, 1996.
- Wagnar H and Bladt S. Plant Drug Analysis. Second edition, Springer-Verlag Publication, Berlin, 1996.
- HPTLC Estimation of Qurecetin in Tylophora indica and Tephrosia purpurea
Abstract Views :673 |
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Authors
Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 5, No 1 (2013), Pagination: 26-29Abstract
Tylophora indica (Family- Asclepiadaceae) having anti-asthmatic, antiinflammatory, sedation decreased motor activity and also useful in allergic rhinitis patients. Tylophora indica contains tylophorine, -amyrin, querceitin, and other major alkaloids like tylophorinine, tylophorinidine and dehydrotylophorine. Tephrosia purpurea Linn (Family- Fabeaceae) having anti-hepatotoxic activity, CNS depressant activity and it also show antiallergic activity. The plant Tephrosia purpurea contains rutin, quercetin, rotenoids, rotenone, tephrosin and lupeol. The powdered aerial part of Tylophora indica and Tephrosia purpuria were extracted with 95% ethanol by hot continuous extraction method individually. The prepared extracts were subjected to HPTLC analysis. The marker compounds were estimated by using pre-coated HPTLC aluminium silica gel 60 F254 plates (MERCK) and compared with standard. The methanolic solution of standard quercetin 5 μl (1 mg/ ml), Tephrosia purpuria extract and Tylophora indica extract 8 μl each (10 mg/ ml) were applied as 7mm band width using C AMAG Linomat IV applicator. The Mobile phase is ethyl acetate: formic acid: glacial acetic acid: water (100:11:11:26). The detection was carried out at 366 nm. The amount of quercetin was estimated by the comparing the peak area of standard and the same was present in the extracts. The content of quercetin in extracts was found to be 4.30% w/w and 1.56% w/w for Tylophora indica extract and Tephrosia purpuria extract respectively. This estimation technique is very much useful for the estimation of quercetin present in the various medicinal plants.Keywords
Quercetin, HPTLC, Tylophora indica and Tephrosia purpuria.References
- R. Aguilar-Sanchez, F. Ahuatl-Garca, M.M. Davila-Jimenez, M.P. Elizalde-Gonz´alez, M.R.G. Guevara-Villa, Chromatographic and electrochemical determination of quercetin and kaempferol in hytopharmaceuticals. Journal of Pharmaceutical and Biomedical Analysis. 38; 2005: 239–249.
- Md. Sarfaraj Hussain, Sheeba Fareed, Mohammad Ali, Hyphenated chromatographic analysis of bioactive gallic acid and quercetin in Hygrophila auriculata (K. Schum) Heine growing wildly in marshy places in India by validated HPTLC method. Asian Pacific Journal of Tropical Biomedicine. 2012: S477- S483.
- M. Ali and K. K. Bhutani, Alkaloids From Tylophora indic. Phytochemistry. 28 (12); 1989: 3513-3517.
- T. Ganguly1, L. P. Badheka2 and K. B.Sainis, Immunomodulatory effect of Tylophora indica on Con A induced lymphoproliferation. Phytomedicine. 8(6); 2001: 431–437.
- V.U. Ahmad, Z. Ali, S.R. Hussaini, F. Iqbal, M. Zahid, M. Abbas, N. Saba, Flavonoids of Tephrosia purpurea. Fitoterapia. 70; 1999: 443-445.
- A.S. Damre, A.B. Gokhale, A.S. Phadke, K.R. Kulkarni, M.N. Saraf, Studies on the immunomodulatory activity of flavonoidal fraction of Tephrosia purpurea. Fitoterapia. 74; 2003: 257–261.
- Seth P. D. HPTLC. First edition, CBS Publishers and distributors, New Delhi. 1996.
- Wagnar H and Bladt S. Plant Drug Analysis. Second edition, Springer-Verlag Publication, Berlin, 1996.
- Development of UV Spectroscopy Fingerprinting Method of Total Polyphenols as Tannic Acid in Manibhadra yoga
Abstract Views :219 |
PDF Views:1
Authors
Affiliations
1 Vikram University, Ujjain (M. P.), IN
1 Vikram University, Ujjain (M. P.), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 4, No 1 (2014), Pagination: 28-30Abstract
Ayurvedic formulation is essential in order to assess the quality of drugs, based on the concentration of their active principles. The main objective of the present study is to develop a simple, rapid and validated UV fingerprint method for estimation of total polyphenols as tannic acid in Manibhadra yoga and crude drugs. Tannic acid is one of the major constituent of Manibhadra yoga and it is official in Ayurvedic formulary of India and it is the most common formulation used for respiratory disorders in Ayurvedic medicinal preparation. It content of the well documented medicinal important plants Embelia ribe (Vidanga), Embelica officinalis (Amalaki), Terminalia chebula (Haritaki), Operculina turpethum (Nishotha) and guda (jeggary). The detection wavelength of tannic acid was 277.2 nm. This method is used for the estimation of tannic acid in formulations and identifies and assessed its quality by using UV method. The estimation was carried out with three laboratory batches and one marketed formulation.Keywords
Manibhadra yoga, UV, Tannic Acid, Ayurvedic.- Spectrophotometric Estimation of Total Polyphenols as Tannic Acid in Ayurvedic Formulation Astangavaleha
Abstract Views :191 |
PDF Views:2
Authors
Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M. P), IN
1 Institute of Pharmacy, Vikram University, Ujjain (M. P), IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 4, No 1 (2014), Pagination: 42-44Abstract
Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The main objective of the present study is to develop a simple, rapid and validated UV fingerprint method for estimation of total polyphenols as tannic acid in Astangavaleha and crude drugs. Astangavaleha is official in Ayurvedic formulary of India and it is the most common formulation used for respiratory disorders in Ayurvedic medicinal preparation. It Composition of the medicinal important plants Myrica esculenta, Inula racemosa, Pistacia integerrima, Trachyspermum ammi, Carum carvi, Zingiber officinale, Piper nigrum, Piper longum, madhu and Zingiber officinale (svarasa). The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 277.2 nm.Keywords
Astangavaleha, UV, Tannic Acid, Ayurvedic.- Spectrophotometric Fingerprinting Method for Unani formulation Hab-e-Azarakhi
Abstract Views :223 |
PDF Views:1
Authors
Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
Source
Asian Journal of Pharmacy and Technology, Vol 2, No 1 (2012), Pagination: 1-3Abstract
Hab-e-Azarakhi is an important Unani formulation, is official in Unani Pharmacopoeia (formulary of Unani Medicine). The formulation is dispensed for the tone up the nervous system and liver. It is also useful for increase the appetite. Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Traditional medicines/herbal formulations. Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. The present study is an attempt to develop the fingerprint method for Hab-e-Azarakhi by spectrophotometric determination using Piperine as a standard, which is as an important content in formulation. The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 342.6 nm. The UV spectrophotometric analysis, which is a simple, precise, and accurate method that can be considered as one of the quality control method for routine analysis.Keywords
Hab-e-Azarakhi, Piperine, Ethanol, Spectrophotometer, Unani Formulation.- Development of Fingerprinting Method for Amlakyadi Churna:Spectrophotometric Approach
Abstract Views :189 |
PDF Views:0
Authors
Affiliations
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
1 Institute of Pharmacy, Vikram University, Ujjain (M.P.), IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 4, No 2 (2012), Pagination: 61-63Abstract
Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. A simple, rapid and valid fingerprint method has been first carried out for estimation of piperine in Amlakyadi churna an herbal formulation. Amlakyadi churna is an important ayurvedic formulation, is official in Ayurvedic formulary of India is combination of four reputed herbs, comprised of the fruits Embelica officinalis, Piper longum, Terminalia chebula and ischolar_mains of Plumbago zylenica and Sendha namak. The formulation is dispensed for the treatment of digestive impairment, fever and dyspepsia. The estimation was carried out with three laboratory batches and one marketed formulation by spectrophotometric approach at 342.6 nm.Keywords
Amlakyadi Churna, Piperine, Ethanol, Spectrophotometer.- Development of fingerprinting method for Siddha formulation Nilaavaarai chooranam: A HPTLC approach
Abstract Views :65 |
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Authors
Affiliations
1 Chhattisgarh Food and Drug Administration, Mahasamund (CG)., IN
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., IN
3 Institute of Pharmacy, Vikram University, Ujjain (MP)., IN
1 Chhattisgarh Food and Drug Administration, Mahasamund (CG)., IN
2 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.)., IN
3 Institute of Pharmacy, Vikram University, Ujjain (MP)., IN
Source
Asian Journal of Research in Chemistry, Vol 15, No 5 (2022), Pagination: 327 - 330Abstract
Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. Fingerprint method for Nilaavaarai Chooranam with TLC Densitometric Methods (HPTLC) using piperine as an internal standard was developed. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation of Nilaavaarai Chooranam and crude drug containing piperine. The concentration of piperine present in raw material was found to be 2.9836±0.497w/w in Piper nigrum and in three identical laboratory batch of Nilaavaarai Chooranam NC-I, NC-II and N-III, was found to be 0.1761 ± 0.384, 0.1759 ± 0.534%, 0.1760 ± 0.019 w/w respectively. The piperine content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Nilaavaarai Chooranam.Keywords
Nilaavaarai chooranam, Piperine, Sidhha formulation, HPTLC, Standardization.References
- Jain V., Saraf S. and Saraf S. Spectrophotometric determination of Piperine in Trikatu Churna: An Ayurvedic Formulation, Asian Journal of Chemistry. 2007, 19, (7), 5331-5335.
- World Health Organization, Quality Control Methods for Medicinal Plants Materials, Geneva, 1998, 1-15.
- WHO general guidelines for methodologies on research and evaluation of traditional medicine. (2000): http://whqlibdoc.who.int/hq/2000/WHO_EDM_TRM_2000.1.pdf.
- Gupta M., Chaudhary P.H., Mukund G. Shrivastava T.B., Need and scope of standardization of herbal medicines - A review, International Journal of Green Pharmacy. Oct-Dec 2021 ,16 (1), 346-352.
- Formulary of Siddha Medicine. 4th ed., Published by Indian Medical Practitioners Cooperative Pharmacy and Store Ltd (IMPCOPS), Chennai, 1993: 45.
- Indian Herbal Pharmacopoeia, Regional Research Laboratory Jammu, Indian drug Manufacturing Association Mumbai. 1999.
- Gurinderdeep S, Piperine: A Remarkable Marker with Intense Biological Activity, International Journal of Pharmacognosy and Chinese Medicine. 2017, 1(4): 122.