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Tanaka, K.
- The Usefulness of Comet Assay in Predicting Hematopoietic Disorders such as Myelodysplastic Syndrome and Acute Myeloid Leukemia
Abstract Views :309 |
PDF Views:71
Authors
Affiliations
1 Department of Biology, Eritrea Institute of Technology, Mainefhi, ER
2 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan, JP
3 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JP
1 Department of Biology, Eritrea Institute of Technology, Mainefhi, ER
2 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan, JP
3 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, JP
Source
Indian Journal of Science and Technology, Vol 1, No 1 (2007), Pagination: 1-4Abstract
The usefulness of 'comet assay' in predicting the hematopoietic disorders such as MDS and AML is reported. Bone marrow biopsies of de novo AML and MDS (3 from each) were subjected to alkaline comet assay along with suitable control. The results are based on the average tailmoment of about hundred randomly selected cells. Blood malignant samples showed significantly higher mean tailmoment (AML: 195±41.6 & MDS: 501±136.2) than the normal (29.66±6.1). In addition, the spontaneous occurrence of apoptotic cells was much higher in MDS (24.5% ±5); while in AML it was <1%. 'Comet assay' offers as single-step visual method to predict the hematopoietic neoplasia in suspected samples.Keywords
Myelodysplastic Syndrome, Acute Myeloid Leukemia, Comet Assay, ApoptosisFull Text
References
- Aul C, Bowen DT and Yoshida Y (1998) Pathogenesis, etiology and epidemiology of myelodysplastic syndromes. Haematologica 83, 71-86.
- Bai A, Kojima H, Hori M, Nara N, Komeno T, Hasegawa Y Ninomiya H, Abe T and Nagasawa T (1999) Priming with G-CSF effectively enhances low-dose Ara-Cinduced in vivo apoptosis in myeloid leukemia cells. Exp. Hematol. 27, 259-265.
- Bennet JM, Catovsky D, Daniel MT, Flandrin G, Galton DA, Gralnick HR and Sultan C (1982) Proposals for the classification of the syndromes. Br. J. Haematol . 51, 189-193.
- Bincoletto C, Saad ST, da-Silva ES and Queiroz ML (1999) Haematopoietic response and bcl-2 expression in patients with acute myeloid leukemia. Eur. J. Haematol . 62, 38-42.
- Gajendiran N, Tanaka K and Kamada N (2000) Comet assay to sense neutron ‘fingerprint’. Mutat. Res, 179-187.
- Haase D, Feuring-Buske M, Schäfer C, Schoch C, Troff C, Gahn B, Hiddemann W and Wörmann B (1997) Cytogenetic analysis of CD34+ subpopulations in AML and MDS characterized by the expression of CD38 and CD117. Leukemia 11, 674-679.
- Herry A, Douet-Guilbert N, Morel F, Le Bris M-J, De Braekeleer M (2007) Redefining monosomy 5 by molecular cytogenetics in 23 patients with MDS/AML. Eur. J.Haematol .78, 457–467.
- Mundle SD, Mativi BY, Bagai K, Feldman G, Cheema P, Gautam U, Reza S, Cartlidge JD, Venugopal P, Shetty V, Gregory SA, Robin E, Rifkin S, Shah R and Raza A (1999) Spontaneous downregulation of Fas-associated phosphatase- 1 may contribute to excessive apoptosis in myelodysplastic marrows. Int. J. Hematol . 70, 83-90.
- Olive PL (1999) DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol . 75, 395-405.
- Plata E, Viniou N, Abazis D, Konstantopoulos K, Troungos C, Vaiopoulos G, Meletis J, Kittas C, Pangalos C and Yataganas X (1999) Cytogenetic analysis and RAS mutations in primary myelodysplastic syndromes. Cancer Genet. Cytogen. 111, 124-129.
- Singh NP, McCoy MT, Tice RR and Schneider EL (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 75,184-191.
- Tsoplou P, Kouraklis-Symeonidis A, Thanopoulou E, Zikos P, Orphanos V and Zoumbos NC (1999) Apoptosis in patients with myelodysplastic syndromes: differential involvement of marrow cells in ‘good’ versus ‘poor’ prognosis patients and correlation with apoptosis-related genes. Leukemia. 13, 1554-1563.
- Xu D, Gruber A, Peterson C and Pisa P (1998) Telomerase activity and the expression of telomerase components in acute myelogenous leukemia, Br. J. Haematol . 102, 1367-1375.
- Yashige H, Horiike S, Taniwaki M, Misawa S and Abe T (1999) Micronuclei and nuclear abnormalities observed in erythroblasts in myelodysplastic syndromes and in de novo acute leukemia after treatment. Acta Haematologia 101,32-40.
- Takagi S, Tanaka O, Origasa H and Miura Y (1999) Prognostic significance of magnetic resonance imaging of femoral marrow in patients with myelodysplastic syndromes. J.Clinical Oncol. 17, 277-283.
- Low Amount Expression of Ku 70 Protein Related to Radiation-induced Chromosome Instability and Radiosensitivity in Normal Lymphocytes, and Leukemia and Lymphoma Cell Lines
Abstract Views :743 |
PDF Views:172
Authors
Affiliations
1 Department of Radiobiology, Institute for Environmental Sciences, Rokkasho, Aomori ,039-3213, JP
2 Department of Cancer Cytogenetics, Research Institute for Environmental Sciences, Hiroshima University, Hiroshima, JP
3 Department of Plant Biology & Plant Biotechnology, Presidency College, Chennai-600 005, IN
1 Department of Radiobiology, Institute for Environmental Sciences, Rokkasho, Aomori ,039-3213, JP
2 Department of Cancer Cytogenetics, Research Institute for Environmental Sciences, Hiroshima University, Hiroshima, JP
3 Department of Plant Biology & Plant Biotechnology, Presidency College, Chennai-600 005, IN
Source
Indian Journal of Science and Technology, Vol 6, No 2 (2013), Pagination: 3981-3989Abstract
Previous our experiment showed that B-lymphoid cell lines established from α ray- and γ-ray irradiated lymphocytes had higher chromosome instabilities through many passages than that from non-irradiated lymphocytes. Present protein study revealed that these B-cell lines had low amount of Ku 70 protein expression before and after second irradiation, longer localization of Ku 70 protein in nucleus, longer period of cell cycle delay at G1 or G2 phases and high activity of DNA-PK. The first αray- or γ-ray irradiation had an effect of persisting impaired DNA repair system in cells, which resulted in a decreased repair capacity after the second irradiation. Leukemia and lymphoma cell lines also showed similar characteristics after irradiation to those of the B-cell lines having high chromosome instability. These similarities are suggesting that characteristics on abnormal Ku 70 protein might persist long term and might be related to initiating leukemogenesis and lymphomagenesis as well as that such an ability of DNA repair protein might apply to prediction of radiosensitivity of leukemia or lymphoma cells.Keywords
DNA Repair, Ku 70 Protein, Chromosome Aberration, Radiosensitivity, Radiation, Chromosome Instability, Leukemia and Malignant LymphomaFull Text
References
- Ader I, Muller C, Bonnet J, Favre G, Cohen-Jonathan E, Salles B, Toulas C (2002) The radioprotective effect of the 24 kDa FGF-2 isoform in Hela cells is related to an increased expression and activity of the DNA dependent protein kinase (DNA-PK) catalytic subunit. Oncogene 21(42), 6471-6479.
- Allalunis-Turner MJ, Lintott LG, Barron GM, Day RS 3rd, Lees-Miller SP (1995) Lack of correlation between DNAdependent protein kinase activity and tumor cell radiosensitivity. Cancer Res.55 (22), 5200-5202.
- Aso H, Tashiro S, Hamamoto K, Otsuji A, Kita K, Kamada N (1991) Establishment of a human acute myeloid leukemia cell line (Kaumi-1 ) with 8;21 chromosome translocation. Blood 77(9), 2031-2036.
- Asou H, Eguchi M, Suzukawa K, Morishita K, Tanaka K, Date M, Hamamoto K, Kamada N (1996) Establishment of a myeloid leukemia cell line (Kasumi-4) with t(9;22;11) (q34;q11;q13), inv(3)(q21q26) and the EVI1 gene activation from a patient with chronic myelogenous leukemia in balstic crisis. (1996) Br. J. Hematol. 93, 68-74.
- Bertinato J, Schild-Pouter C, Haché RJ (2001) Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku 70 and Ku 80 subunits. J. Cell Sci. 114 (Pt1), 89-99.
- Beskow C, Skikuniene J, Holgersson A, Nilsson B, Lewensohn R, Kanter L, Viktorsson K (2009) Radioresistant cervical cancer shows upregulation of the NHEJ proteins DNAPKcs, Ku 70 and Ku 86. Br. J. Cancer 101(5) 816-821.
- Giovanetti A, Deshpande T, Basso E (2008) Persistence of genetic damage in mice exposed to low dose of X-rays. Int. J. Radiat. Biol. 84(3), 227-235.
- Gu Y, Jin S, Gao Y, Weaver DT, Alt FW (1997) Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and in ability to support V(D)J recombination. Proc. Natl. Acad Sci. USA 94(15): 8076-8081.
- Hille A, Hofman-Hüther H, Kühnle E, Wilken B, Rave-Fränk M, Schmidberqer H, Virsik P (2012) Spontaneous and radiation- induced chromosomal instabikity and persistence of chromosome aberratins after radiotherapy in lymphocytes from prostate cancer patients. Radiat. Environment. Biophysic. Doi:10.1007/s00411-009-0244-x. citeulike:5810374.
- Koike M, Ikuta T, Miyasaka T, Shiomi T (1999) Ku 80 can translocate to the nucleus independent of the translocation of Ku 70 using its own nuclear localiuzation signal. Oncogene 18(52), 7495-7505.
- Koike M and Koike A (2005) The Ku 70-binding site of Ku 80 is required for the stabilization of Ku 70 in the cytoplasm, for the nuclear translocation of Ku 80, and for Ku 80-dependent DNA repair. Exp. Cell Res. 305(2), 266-276.
- Komuro Y, Watanabe T, Hosoi Y, Matsumoto Y, Nakagawa K, Tsuno N, Kazama S, Kitayama J, Suzuki N, Nagawa H (2002) The expression pattern of Ku correlates with tumor radiosensitivity and disease free survival in patients with rectal carcinoma. Cancer 95(6): 1199-1205.
- Kumaravel TS, Bhrathy K, Kudoh S, Tanaka K, Kamada N (1998) Expression, localization and functional interactions of Ku 70 subunit of DNA-PK in peripheral lymphocytes and Nalm-19 cells after irradiation. Int. J. Radiat. Biol. 74, 481- 489.
- Marangoni E, Le Romancer M, Muller C, Douc-Rasy S, Vaganay S, Abdulkarim B, Barrois M, Calsou P, Bermier J, Salles B, Burhis J. (2000) 7(2), 339-346. Transfer of Ku86 RNA antisense decreases the radioresistance of human fibroblasts. Cancer Gene Ther. 7(2): 339-346.
- Muller C and Salles B (1997) Regulation of DNA-dependent protein kinase activity in leukemic cells. Oncogene 15, 2343-2348.
- Muller C, Christodoulopoulos G, Salles B, Panasci L (1998) DNA-dependent protein kinase activity correlate with clinical and in vitro sensitivity of chronic lymphocytic leukemia lymphocytes to nitorogen mustards. Blood 92(7): 2213-2219.
- Nagai M, Fujita M, Ikeda T, Ohmori M, Kuwabara H, Yamaoka G, Tanaka K, Kamada N, Taniwaki M, Inoue T, Irino S, Tahara J (1997) Alterations of p53 and Rb genes in a novel human GM-CSF-dependent myeloid cell line (OHNGM) established from therapy-related leukemia. Br. J. Hematol. 98, 392-398.
- Nagasawa H, Little JB, Lin Yu-F, So S, Kurimasa A, Peng Y, Brogan JR, Chen DJ, Bedford JS, Chen PC. (2011) Differential role of DNA-PKcs phosphorylations and kinase activity in radiosensitivity and chromosomal instability. Radiat. Res., 175: 83-89. ref no.83-89.
- Negroni A, Strnati L, Grollino MG, Gumiero D, Danesi DT (2008) Radioresistance in a tumour cell line correlates with radiation inducible Ku 70/80 end-binding activity. Int. J. Radiol. Biol. 84(4): 265-276.
- Nimura Y, Kwata T, Uzawa K, Okamura J, Liu C, Saito M, Shimada H, Seki N, Nakagawara A, Ito H, Ociai T, Tanzawa H (2007) Silencing Ku80 using small interfering RNA enhanced radiation sensitivity in vitro and in vivo. Int J. Oncol. 30(6): 1477-1484.
- Pavôn MA, Parreňo M, Leôn X, Sancho FJ, Cèspedes MV, Casanova I, Lopez-Pousa A, Mangues MA, Quer M, Barnadas A, Manques R. Ku70 predicts response and primary tumor recurrence after therapy in locally advanced head and neck cancer. Int J. Cancer. 123(5): 1068-1079.
- Plappert UG, Stocker B, Fender H, Fliedner TM (1997) Changes in the repair capacity of blood cells as a biomarker for chronic low-dose exposure to ionizing radiation. Environ. Mol.. Mutagenesis 30(2), 153-160.
- Shintani S, Mihara M, Li C, Nakahara Y, Hino S, Nakashiro K, Hamakawa H (2003) Up-regulation of DNA-dependent protein kinase correlates with radiation resistance in oral squamous cell carcinoma. Cancer Sci. 94(10):894-900.
- Someya M, Sakata K, Monobe M, Ikebe H, Ando K, Hosoi Y, Szuki N, Hareyama M (2006) The association of DNAdependent protein kinase activity with chromosomal instability and risk of cancer. Carcinogenesis 27, 117-122.
- Someya M, Sakara K-I, Matsumoto Y, Kamdar RP, Kai M, Toyota M, HAreyama M (2011) The association of DNAdependent protein kinase activity of peripheral blood lymphocytes with prognosis of cancer. Br. J. Cancer104, 1724- 1729.
- Suzuki K, Ojima M, Kodama S, Watanabe M (2003) Radiation-induced DNA damage and delayed induced genomic instability. Oncogene 22, 6988-6993.
- Tanaka K, Kumaravel TS, Ihda S, Kamada N (2008a) Characterization of late-arizing chromosome aberrations in human B-cell lines established from α-ray- or γ-ray-irradiated lymphocytes. Cancer Genet. Cytogenet. 187, 112-124.
- Tanaka K and Ihda S (2008b) Radiation-induced chromosome instability and leukemogenesis un human. J. Genet. Toxicol. 1(1), 1-19. http:/www.genetox.net
- Urano M, He F, Minami A, Ling CC, Li GC (2010) Response to multiple radiation doses of human colorectal carcinoma cells infected with recombinant-negative Ku70 fragments. Int J. Radiat, Oncol. Phys. 77(3): 877-885.
- Wang X, Szabo C, Qian C, Amadio PG, Thiboreau SN, Cerhan JR, Petersen GM, Liu W, Couch FJ (2008) Nutational analysis of twenty-two double-strand DNA break repair genes in breast and pancreatic cancers. Cancer Res. 68(4), 971-975.
- Ye J, Ren Z, Gu Q, Wang L, Wang J (2011) Ku 80 is differentially expressed in human lung carcinomas and upregulated in response to irradiation I mice. DNA Cell Biol. 30(12), 987-994.