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K. Subha ¹, K. Kanimozhi ², A. Panneerselvam ¹

Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi 613 503, Thanjavur, Tamil Nadu, IN
2 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi 613 503, Thanjavur, Tamil Nadu

Abstract

In the present study soil sample were collected from salt pan region of Vedharanyam, Nagapattinam District, Tamilnadu. The physico-chemical characteristics of soil samples were found to affect the distribution of population of fungi. The fungal diversity from the soil sample were analysed by plating technique. Fungi isolation was done by the soil dilution method incubated at 26±2°C for 3-5 days. The predominant fungi isolated include; Asprgillus flavus, A. terreus, A. sydowi, A. oryzae, A. ruber, A. sulphureus, A. conicus, A. chevalieri, A. flavipes, Absidia glauca, Acrophialophora fusispora, Gliocladium sagariensis, Geotrichum candidum, Penicillium citrinum, P. chrysogenum, P. purpurogenum, Trichoderma harzianum, T. koningii, T. viride and Alternaria alternata.

Keywords

Biodiversity, Fungi, Salt Pan Soil, Coastal, Physico-Chemical.

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S. Deepa ¹, K. Kanimozhi ², A. Panneerselvam ²

Affiliations
1 P.G. Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous) Poondi-613503, Thanjavur Dist, Tamil Nadu, IN
2 P.G. Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous) Poondi-613503, Thanjavur Dist, Tamil Nadu, IN

Abstract

The soil sample was collected from the salt pan of Bay of Bengal at Vedaranyam,Nagapattinam (Dt.) Tamilnadu, India. The physico-chemical parameters at the sample were analyzed by using standard procedures.Totally 10 actinomycetes were isolated and identified from the salt pan soil sample. Two actinomycetes namely Jonesia denitrificans and Nocardia amarae were dominant in their growth out of 10 isolated actinomycetes.

Antibacterial activity of Jonesia denitrificans and Nocardia amarae were studied against five human bacterial pathogens such as Salmonella typhi, Bacillus subtilis, Staphylococcus aureus, Vibrio cholerae and Klebsiella pneumonia. The acetone extract of Nocardia amarae exhibited promising antibacterial activity against Bacillus subtilis (25 mm). The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics.

Keywords

Antibacterial Activity, Marine Actinomycetes, Jonesia denitrificans.

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S. Deepa ¹, P. Bhuvanehswari ², K. Kanimozhi ¹, A. Panneerselvam ¹

Affiliations
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College, (Autonomous), Poondi, Thanjavur, IN
2 Department of Microbiology, A.V.C College (Autonomous), Mannampandal, Mayiladuthurai, IN

Abstract

Alcaligenes eutrophus was collected from MTCC bank and purity was checked according to the guidelines of Bergey's manual of bacteriology. The PHB production ability of the culture was tested by "Granular stains method". The test organism was grown in synthetic and various industrial waste based medium (Molasses, seasame oil waste) at different concentration (10%, 20%, 30% and 40%).The PHB producer grown in synthetic and various industrial waste based medium and PHB was extracte and estimated by using UV spectrophotometer. The pure form of PHB powder was collected and studied by Infrared analysis. In the present study, the high PHB production was seen in Alcaligenes eutrophus grown in 40% concentration of seasame oil waste when compared with other substrate and synthetic medium. The present study concludes that the seasame oil waste based medium and Alcaligenes eutrophus used for PHB production in large scale that minimize the cost of the product. Thus we can minimize the use of petroleum-derived plastics and by which we can live in a plastic pollution free earth.

Keywords

PHB, Molasses, Seasame Oil Waste, Alcaligenes eutrophus.

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K. Kanimozhi ¹, A. Panneerselvam ¹

Affiliations
1 Dept of Botany and Microbiology, A. V. V. M. Sri Pushpam College (Autonomous), Poondi- 613 503, Thanjavur Dt , Tamilnadu, IN

Abstract

Asymbiotic, associative, or symbiotic, Nitrogen fixing strains of Azospirillum isolated from Rhizosphere soils of paddy field. Totally 30 Azospirillum strains were isolated from paddy field of Thanjavur District by using Nitrogen free bromothymol blue (Nfb) medium. The isolated strains were characterized by physiological, morphological and biochemical characteristics and construct the phylogenetic tree. Based on this characterization, among the 30 isolates, nine belonged to A. halopraferens, 8 belonged to A. amazonense, 6 belonged to A. brasilense, 4 belonged to A. lipoferm and 3 belonged to A. irakense.

Keywords

Morphological, Physiological, Biochemical Character, Azospirillum Spp.

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K. Kaviyarasi ¹, K. Kanimozhi ¹, P. Madhanraj ², A. Panneerselvam ¹, V. Ambikapathy ¹

Affiliations
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN

Abstract

The soil sample was collected from Manora. After analysing the physic-chemical parameters of the collected marine soil sample, ten actinomycetes species including Actinobispora yunnanensis, Streptomyces albus,Micromonospora echinospora, Saccharopolyspora hirsute, Streptomycetes cyaneus, Actinomadura citrea, Saccharomonospora viridis, Thermomonospora mesophila, Streptoverticillium album Microtetrospora fastidiosa were isolated and they were screened for phosphate solubilization using Pikovskaya's agar medium and the Actinobispora yunnanensis was found to be dominant in phosphate solubilization. Then the DNA of the Actinobispora yunnanensis was isolated, amplified and its 16S rDNA was sequenced and its secondary structure was also predicted.The phylogenetic analysis of the Actinobispora yunnanensis was also carried out.

Keywords

Phosphate, Phosphate Solubilization, 16S r DNA, Actinobispora yunnanensis.

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