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Nataraja, K. N.
- Full-Length Cloning and Characterization of Abiotic Stress Responsive CIPK31-Like Gene from Finger Millet, a Drought-Tolerant Crop
Abstract Views :321 |
PDF Views:181
Authors
K. N. Nagarjuna
1,
M. S. Parvathi
1,
R. S. Sajeevan
1,
V. Pruthvi
1,
H. M. Mamrutha
2,
K. N. Nataraja
1
Affiliations
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
Source
Current Science, Vol 111, No 5 (2016), Pagination: 890-894Abstract
Regulatory proteins such as protein kinases are known to play a crucial role in plant stress acclimation pathways. In this study, we report the identification and characterization of an abiotic stress responsive protein kinase called CBL Interacting Protein Kinase (EcCIPK31-like) from finger millet, a drought-tolerant crop. PCR-based approach was followed to clone 1350 bp coding region that encodes a 449 amino acid protein with a calculated molecular mass of 50.69 kDa. The conserved domain analysis revealed the presence of CIPK domain with 90% sequence similarity with fox tail millet SiCIPK31-like gene. We also report its upregulation under salinity, desiccation, oxidative and temperature stresses at seedling level in finger millet. Drought stress at wholeplant level significantly induced the expression of EcCIPK31-like, indicating that the gene is linked to drought signalling pathways. The stress-responsive nature of EcCIPK31-like to diverse stresses indicates that the gene could regulate multiple cellular tolerance traits and its further functional validation can highlight the relevance in abiotic stress acclimation in plants.Keywords
Abiotic Stress Signalling, Finger Millet, Full-Length Cloning, Protein Kinase.Full Text
- Leaf Surface Wax Composition of Genetically Diverse Mulberry (Morus sp.) Genotypes and its Close Association with Expression of Genes Involved in Wax Metabolism
Abstract Views :461 |
PDF Views:154
Authors
H. M. Mamrutha
1,
K. N. Nataraja
2,
N. Rama
2,
D. K. Kosma
3,
T. Mogili
4,
K. Jhansi Lakshmi
5,
M. Udaya Kumar
2,
M. A. Jenks
6
Affiliations
1 Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
2 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
3 Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, US
4 Central Sericultural Research and Training Institute, Mysuru 570 008, IN
5 Central Sericultural Germplasm Resources Centre, Thally Road, Hosur 635 109, IN
6 Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, US
1 Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
2 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
3 Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, US
4 Central Sericultural Research and Training Institute, Mysuru 570 008, IN
5 Central Sericultural Germplasm Resources Centre, Thally Road, Hosur 635 109, IN
6 Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, US
Source
Current Science, Vol 112, No 04 (2017), Pagination: 759-766Abstract
Silkworm (Bombyx mori), the primary producer of silk, has strong feeding preference for most turgid and hydrated mulberry leaves. In a previous study, we showed positive correlation between moisture retention capacity (MRC) of the mulberry leaf and leaf surface wax amount. In the present study, we examined wax constituents in genotypes that exhibited a wide range of leaf surface wax amount and MRC. Gas chromatographic analysis revealed that acids, alkanes, aldehydes, primary alcohols, iso-alkanes, triterpenoids, esters were among mulberry waxes identified and the major being alkanes. The highest total leaf wax amount was 1006.8 μg dm-2 in the V1 genotype whereas S-36 had the least wax at 436.9 μg dm-2. The alkanes were dominated by those having 25, 27, 29, 31 and 33 carbons, with C31 compounds being the most abundant. RNA-blot hybridization performed with 13 wax biosynthesis associated genes using heterologous probes revealed a close association between surface wax composition and expression levels of genes associated with wax elongation (CUT1, KCS1). The association was also established with homologous probes of KCS1, WAX2, CUT1 and LTP1-like genes. In summary, regulation of early wax precursor metabolism is a likely contributor to the variation observed in leaf wax composition in mulberry.Keywords
Cuticular Wax, Moisture Retention Capacity, Mulberry, Silkworm, Wax Genes.Full Text
- Endophytic Fungi of Salt Adapted Ipomea pes-caprae L. R. Br: their Possible Role in Inducing Salinity Tolerance in Paddy (Oryza sativa L.)
Abstract Views :409 |
PDF Views:171
Authors
Affiliations
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
Source
Current Science, Vol 118, No 9 (2020), Pagination: 1448-1453Abstract
Endophytic fungi occur ubiquitously in all plants. Though their origin and evolution is enigmatic, they are known to play an important role in plant growth and development. Here we explore the endophytic fungal diversity of a perennial cree ping vine, Ipomea pes-caprae (family Convolulaceae), occurring naturally in the coastal sand dunes of peninsular India. Of the ten endophytes isolated from the plant, Fusarium oxysporum (MH511104) was found to grow even at 2 M NaCl in potato dextrose agar medium. The fungus was able to successfully colonize and impart salinity tolerance to salt -sensitive paddy variety, IR-64. We discuss these results in the context of increasing global interest on endophytes as a possible alternative route to crop i mprovement.Keywords
Endophytic Fungi, Ipomea pes-caprae, Paddy, Salt Stress.Full Text
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- Rehydration Induces Early and Rapid Bud Break in Drought Stressed Mulberry Plants
Abstract Views :364 |
PDF Views:148
Authors
Affiliations
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
Source
Current Science, Vol 119, No 7 (2020), Pagination: 1085-1086Abstract
No Abstract.Keywords
No Keywords.Full Text
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