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Authors
Affiliations
1 Department of Crop Physiology, University of Agricultural Sciences,
GKVK, Bengaluru 560 065, IN
2 ICAR-Indian Institute of Wheat and Barley Research, Karnal 132 001, IN
Source
Current Science, Vol 111, No 5 (2016), Pagination: 890-894
Abstract
Regulatory proteins such as protein kinases are known to play a crucial role in plant stress acclimation pathways. In this study, we report the identification and characterization of an abiotic stress responsive protein kinase called CBL Interacting Protein Kinase (EcCIPK31-like) from finger millet, a drought-tolerant crop. PCR-based approach was followed to clone 1350 bp coding region that encodes a 449 amino acid protein with a calculated molecular mass of 50.69 kDa. The conserved domain analysis revealed the presence of CIPK domain with 90% sequence similarity with fox tail millet SiCIPK31-like gene. We also report its upregulation under salinity, desiccation, oxidative and temperature stresses at seedling level in finger millet. Drought stress at wholeplant level significantly induced the expression of EcCIPK31-like, indicating that the gene is linked to drought signalling pathways. The stress-responsive nature of EcCIPK31-like to diverse stresses indicates that the gene could regulate multiple cellular tolerance traits and its further functional validation can highlight the relevance in abiotic stress acclimation in plants.
Keywords
Abiotic Stress Signalling, Finger Millet, Full-Length Cloning, Protein Kinase.
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