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Sailaja, Inampudi
- Antioxidant Activity in Piper Betel and Nicotiania Tabaum
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Authors
Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S., Loni (M.S.), IN
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S., Loni (M.S.), IN
Source
The Asian Journal of Experimental Chemistry, Vol 4, No 1-2 (2009), Pagination: 70-73Abstract
The aqueous extract of Piper betel L. and Nicotiania tabaum were studied for antioxidant activity on different in vitro models namely 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) assay, nitric oxide assay and trichloroacetic acid based reducing power method. Ascorbic acid was also evaluated for comparison. The extracts showed free radical scavenging property in the tested models. Piper betel L. showed 98.06% inhibition of DPPH at 1000 μg and its activity at 500 μg (i.e. 94.35%)was comparable to that of ascorbic acid at 30μg (93.58%). While the maximum percentage inhibition by Piper betel L. and Nicotiania tabaum in the nitric oxide model was found to be only 62.14% and 33.36%, respectively, the activity of 1024 μg of Nicotiania tabaum and 128μg of Piper betel L. of compares favorably with that of 30 μg ascorbic acid. Piper betel L. showed high reductive ability. This study demonstrates the higher anti oxidant activity is present in the leaves of Piper betel L. when compared to Nicotiania tabaum.Keywords
Antioxidant, Piper betel L. and Nicotiania tabaum, Free Radicals, Reductive Ability.- Study of Oxidative Stress and Antioxidant Status of X-Ray Technicians Exposed to Low Radiation Doses During Their Occupational Exposure in Kanchipuram District of Tamil Nadu
Abstract Views :164 |
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Authors
Affiliations
1 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN
2 Department of Pharmacology, Chalmeda Anand Rao Institute of Medical Sciences, Bommakal Karimanagar (T.N.), IN
3 Department of Biotechnology, College of Agricultural Biotechnology, Loni (M.S.), IN
4 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN
1 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN
2 Department of Pharmacology, Chalmeda Anand Rao Institute of Medical Sciences, Bommakal Karimanagar (T.N.), IN
3 Department of Biotechnology, College of Agricultural Biotechnology, Loni (M.S.), IN
4 Department of Biochemistry, Melmaruvathur Adhiparasakthi Institute of Medical Sciences, Melmaruvathur (T.N.), IN
Source
International Journal of Medical Sciences, Vol 4, No 1-2 (2011), Pagination: 52-55Abstract
Free radicals especially the reactive oxygen species (ROS) implicated in etiology for over hundred diseases. Radiation is one of the major exogenous sources of free radicals in man and it has been proved that ionizing radiation produces ROS in biological system capable of destroying biomolecules. The objective of this study was to measure the serum antioxidant status level in health workers, to estimate the oxidative stress in red blood cells of radiographers by measuring the malondialdehyde (MDA) and percentage hemolysis of RBCs in comparison with controls, to determine the concentrations of antioxidants like vitamin E and catalase in the above groups, to determine the relationship between the levels of oxidative stress, antioxidants and the duration of occupational exposure in radiographers. 5ml of venous blood was collected in heparin. Plasma was used for estimation of vitamin E. The separated cells were used for the assay of oxidative stress parameters - malondialdehyde -oxidative hemolysis of RBS's (or) per cent of hemolysis of RBC, antioxidant status parameters- vitamin E and catalase. Present results show that the percentage of hemolysis of RBC was higher in, radiographers when compared with control group (p=0.0059). The RBC MDA (p=0.690), Catalase (p=0.050) and plasma vitamin 'E' levels (p=0.50) were higher in radiographers than in control group. Finally we can conclude that there is a mild increase in oxidative stress occurring as a result of chronic occupational exposure to low dose ionizing radiation.Keywords
MDA, ROS, Vitamin E, Catalase and Oxidative Stress.- Antioxidant Activity in Ocimum sanctum Linn, Ocimum Bascilicum
Abstract Views :203 |
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Authors
Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni, Ahmednagar (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S, Loni, Ahmednagr (M.S.), IN
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni, Ahmednagar (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S, Loni, Ahmednagr (M.S.), IN
Source
Asian Journal of Bio Science, Vol 5, No 2 (2010), Pagination: 195-199Abstract
Ocimum sanctum Linn., Ocimum bascilicum Linn., which have been normally used in traditional puja at every Indian Hindu home, was estimated by three different methods: ferric reducing antioxidant power (FRAP) assay, improved ABTS radical cation decolorization assay and DPPH free radical scavenging assay. Additionally, their total phenolic contents were analyzed by Folin- Ciocalteau micro method. The result showed that Ocimum bascilicum seemed to be better source of antioxidant compounds, followed by Ocimum sanctum. Total phenolic content analysis, were significantly related to those of FRAP, ABTS, DPPH.Keywords
Antioxidant Status, Total Phenolic Content, O.bascilicum, O.sanctum.- Antioxidant Activity and Phenolic Contents in Ocimum sanctum and Ocimum bascilicum
Abstract Views :246 |
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Authors
Affiliations
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni, Ahmednagar, (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S, Loni, Ahmednagar, (M.S.), IN
1 Department of Biochemistry and Molecular Biology, College of Agricultural Biotechnology, Loni, Ahmednagar, (M.S.), IN
2 Department of Biochemistry, Rural Medical College, P.I.M.S, Loni, Ahmednagar, (M.S.), IN