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Miftachul Jannah ¹, Imam Mustofa ², Suzanita Utama ², Sri Mulyati ², Ismudiono ², Erma Safitri ³, M. Anam Al-Arif ⁴

Affiliations
1 Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, IN
2 Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, ID
3 Universitas Airlangga, Kampus C Unair, Jl. Mulyorejo, ID
4 Laboratory of Department Animal Husbandry, Universitas Airlangga, Kampus C Unair, Jl. Mulyorejo, ID

Abstract

This study aimed to determine the effect of Milk Urea Nitrogen (MUN) levels as an indicator of protein intake to estrogen levels and pregnancy status in dairy cows. Eighteen productive heifers from wagir district, Malang were collected out from criteria. Thus, they were groups based on MUN levels and reproductive status. Feeding is done in the same pattern. Blood drawing for estrogen level were analized by Enzyme Linked Immunosorbent Assay (ELISA). Milk extraction for analysis of urea levels using the Barthelot method. Pregnancy examinations were carried out three months after Insemination. Data analysis were processed using SPSS (Statistical Package for the Social Sciences) for Windows programs. The results showed that non-pregnant cow had MUN levels from 16-28 mg/dL and high estrogen levels at time of estrus were the same as estrogen levels in pregnant cow. It can be concluded that the same feed pattern different produces MUN levels, estrogen and pregnancy status, depending on the efficiency of nitrogen utilization in the body and physiological health of individual dairy cows.

Keywords

Friesian Holstein, Feed, Estrogen, MUN, Pregnancy.

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M. Ardiansyah ¹, Tita Damayanti ², Tri Wahyu Suprayogi ³, Imam Mustofa ², Ismudiono ⁴, Wurlina ⁵

Affiliations
1 Biology Reproduction and Veterinary Reproduction, Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID
2 Laboratory of Obstetrics Veterinary Department Veterinary Reproduction, Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID
3 Laboratory of Insemination Department Veterinary Reproduction, Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID
4 Laboratory of Fisiology Reproduction Department Veterinary Reproduction, Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID
5 Laboratory of Reproduction Disease Department Veterinary Reproduction, Kampus C Unair, Surabaya, ID

Abstract

MUN is high due to high protein content in feed, Thehigh level of MUN can affect hormonal systems including growth hormone IGF-1 and reduce pregnancy rates. The aim of this study is to determine MUN as an indicator of feed evaluation and see the corelation between MUN and IGF-1 levels and pregnancy rates. The cows according to the criteria were sampled to determine the levels of MUN and IGF-1 then processed with the application curve ekspert 1.4 and SPSS 20. Value r = 0.75 between MUN and IGF-1 stated that there was a strong correlation between the two. There were significant differences between MUN and pregnancy rates (P <0.05). High and low levels of MUN will affect pregnancy rates. All sample of MUN with levels of 17-28.5 mg/dl did not have pregnancy and MUN with levels of > 12 mg/dl was obtained in five out of eight pregnant.

Keywords

Milk Urea Nitrogen (MUN), IGF-1 Hormone, Pregnancy Rate, Blood Urea Nitrogen (BUN) and Dairy Cow.

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