Informatics Publishing Limited

Hijrah Asikin ¹, Ninik Mudjihartini ², Sri Widia A. Jusman ², Mohamad Sadikin ², Sarifuddin Anwar ³

Affiliations
1 Master Student in Biomedical Science, ID
2 Center of Hypoxia and Oxydative Stress, ID
3 Department of Anatomy Faculty of Medicine Universitas Tadulako, ID

Abstract

Introduction: Spleen is the largest lymphatic organ in the body that is responsible for initiating an immune response to antigens, consistof macrophages and lymphocytes. Macrophages are cells that engulf foreign bodies by migration, phagocytosis, O₂ burst, lysosomes fusion, synthesis and secretion of various cytokines which would require enormous amounts of energy. Therefore, macrophages needs of O₂ to increase resulting in hypoxia relative. To overcome this situation, the cell is equipped with a special mechanism which is under the control of HIF. They are highly dependent to O₂existence to produce energy. This process is important for biochemical adaptation in homeostasis. Macrophages expressing Cygb for supplying O₂ and PGC‑1 for mitochondrial biogenesis in producing energy. This study aims to determine whether the spleen macrophages in mice being immunized intraperitoneally with SRBC increase the expression of HIF, Cygb and PGC‑1α. Method: 24 male BALB/c mice, aged 2 months were immunized by injecting 0.2 ml SRBC 2%. Macrophages were taken from spleen of mice. Expression of mRNA and protein levels of HIF‑1α, HIF‑2α, Cygb, PGC‑1α in macrophages were measured by real time RT‑PCR and ELISA, respectively. Level of O₂burst were measured with WST Salts. Observations were made at 24, 48, and 72 hours post‑immunization. Results: The ability to oxidize antigen levels (O₂burst) found higher at 24 hours after immunization. The levels of protein and mRNA expression of HIF‑1α and HIF‑2α showed the highest increase at 24 and 48 hours after immunization. The levels of Cygb protein increased at 48 hours group after immunization while mRNA expression increased at 24 hours after immunization. PGC‑1α protein levels decreased in 24 hours after immunization and then increased gradually. Conclusion: This study shows the expression pattern of several proteins in spleen macrophages of mice that was immunized

Keywords

Spleenmacrophages, HIF‑1α, HIF‑2α, Cytoglobin, PGC‑1α, Immunization

Full Text