- N. Ibrahimi
- S. Tufel
- M. Maurya
- H. S. Bhadoria
- A. Singh
- A. Raheja
- R. Sharma
- J. Singh
- T. Kaur
- T. G. Shrivastav
- S. Nara
- V. Tripathi
- Z. Sayeed
- K. Rangari
- S. K. Chaube
- R. Dada
- R. Kumar
- M. B. Shamsi
- A. Varshney
- J. Snighdha
- R. K. Sharma
- V. Prakash
- V. K. Sehgal
- V. K. Bajaj
- A. Gupta
- B. S. Gill
- V. Sehgal
- M. Prasad
- S. K. Singh
- J. S. Sandhu
- P. K. Jain
- N. D. Chogale
- R. D. Bondre
- R. Pai
- S. Y. Metar
- S. B. Satam
- A. D. Adsul
- M. K. Lonare
- M. Sharma
- R. Udheya
- S. Singla
- V. K. Dumka
- Indian Journal of Physiotherapy & Occupational Therapy-An International Journal
- International Journal of Agricultural Engineering
- Journal of Endocrinology and Reproduction
- International Journal of Medical and Dental Sciences
- Current Science
- Manufacturing Technology Today
- The Asian Journal of Animal Science
- Toxicology International (Formerly Indian Journal of Toxicology)
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Singh, H.
- Effect of Sitting Balance Training under Varied Sensory Input on Balance and Quality of Life in Stroke Patients
Authors
1 Jamia Hamdard, IN
2 Neurophysiotherapy HOD AIIMS, New Delhi, IN
Source
Indian Journal of Physiotherapy & Occupational Therapy-An International Journal, Vol 4, No 2 (2010), Pagination: 40-45Abstract
Background
Balance is the most common problem occurs in stroke patients. Most of the work done in balance training is focused on giving balance training once the standing position is achieved. It is also seen that task related balance training with altered sensory input is better than task related training without altered sensory input. So this study is directed toward giving balance training in sitting position under varied sensory for improving balance and quality of life in stroke patients.
Methods
30 subjects of stroke were selected and randomized into an experimental and control group. The experimental group participated in sitting balance training under varied sensory input and control group were given sitting balance training without varied sensory input. Performance of training were measured before and after sitting balance training using berg balance scale, stroke specific quality of life questionnaire and sitting and sit to stand component of motor assessment scale.
Results
After training both experimental and control group improved significantly in balance and quality of life but more significant improvement was seen in experimental group.
Conclusion
Balance training can be started early in rehabilitation program once sitting is achieved with altered sensory input for improving balance and quality of life.
Keywords
Balance, Varied Sensory Input, Sitting Balance TrainingReferences
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- Isabella V Bonen, Alian P Yelnik, et al, Reliance on visual information after stroke. Part II: Effectiveness of a balance rehabilitation program with visual cue deprivation after stroke: A randomized controlled trail. Archives of physical medicine and rehabilitation 2004; 85: 274-278
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- Aprile at al, Effects of rehabilitation on quality of life in patient with chronic stroke. Brain injury 2008; 22(6): 451-456
- Rainfall Probability Analysis and Crop Planning for Chambal Region of Madhya Pradesh
Authors
1 College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya,Gwalior M.P., IN
2 College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior M.P., IN
Source
International Journal of Agricultural Engineering, Vol 6, No 1 (2013), Pagination: 236–239Abstract
Daily rainfall data of 29 years (1981-2009) recorded at RVSKVV, Zonal Agricultural Research Station Morena, Madhya Pradesh was examined for long term averages of annual, seasonal, monthly, and weekly rainfall and its temporal variability. Co-efficient of variation of 27.1 per cent indicated that the annual rainfall was more or less stable over the years. The season-wise per cent contribution of annual rainfall was 3.7, 7.9 and 88.4 per cent of summer, Rabi and Kharif seasons, respectively. With in the rainy season, August was the highest rainfall contributing month (33.4%) followed by July (28.9%) mean weakly, precipitation amount and its assurance reaches the peak (>50mm/week) during 26th SMW (Standard Meteorological Week) to 38th SMW and again declined thereafter. The earliest onset of rainy season occurred in 24th SMW. The normal onset of rainy season was observed as 26th SMW with CV of 5.8 per cent. There is an ample scope for rain water harvesting from July to September which can be utilized as crop saving irrigation as well as pre-sowing irrigation for succeeding Rabi crops which are generally sown on residual soil moistureKeywords
Rainfall Pattern, Probability Analysis, Crop Planning- Happy Seeder - A Conservation Agriculture Technology for Managing Rice Residue for Central Punjab Conditions
Authors
1 Krishi Vigyan Kendra, Shamsher Nagar, Fatehgarh Sahib Punjab, IN
Source
International Journal of Agricultural Engineering, Vol 6, No 2 (2013), Pagination: 355–358Abstract
Field experiments on Happy seeder were conducted during 2009-10 and 2010-11 in farmer participatory research mode at different locations in the district Fatehgarh Sahib. The field experiments were conducted on 9 different locations to evaluate the operational performance of Happy seeder machine in context of heavy soils of district Fatehgarh Sahib, the effect of Happy seeder on wheat yield in heavy soils and to work out the economics of Happy Seeder as compared to farmer's practice. The study reveled that, the average reduction in weed count in happy seeder plots was 28% compared to conventional sown wheat. Wheat yield during these two years in 9 experiments was varied from 35.0 - 56.25 q/ha and 31.75 - 50.75 q/ha for Happy seeder and conventional seed drill plots, respectively with an average increase in yield of 8.84 % in Happy seeder plots.Keywords
Happy Seeder, Wheat Yield, Residue Management, Paddy Residues, Rice-wheat Rotation- Preparation of Horseradish Peroxidase-Carbamide and its Use in Hapten Immunoassays
Authors
1 Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Munirka, New Delhi-110067, IN
2 Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khaus, New Delhi, IN
Source
Journal of Endocrinology and Reproduction, Vol 11, No 2 (2007), Pagination: 69-75Abstract
Preparation of horseradish peroxidase (HRP) carbamide that is HRP linked to urea (HRP-carbamide/HRP-U) is demonstrated and its potential application in the development of enzyme immunoassays (EIAs) for haptens is described. In this new strategy, the lysine residues of HRP were acylated and then acylated HRP was activated to create highly reactive functional groups by periodate oxidation of its carbohydrate moiety and, subsequently, forming a peptide bond with one of the amino groups of urea. The resulting HRP-carbamide was then coupled to carboxylic derivatives of cortisol-21-hemisuccinate (F-21-HS), 17α-OH progesterone-3-O-carboxymethyl-oxime (17α-OHP-3-O-CMO) and nandrolone-3-O-carboxymethyl-oxime (N-3-O-CMO) to prepare enzyme conjugates adopting N-hydroxysuccinimide-carbodiimide method. The F-21-HS-U-HRP, 17α-OHP-3-O-CMO-U-HRP, and N-3-O-CMO-U-HRP enzyme conjugates thus prepared were used for the development of an enzyme linked immunosorbent assays (ELISAs) for the estimation of cortisol, 17α-OHP and nandrolone. The sensitivity of cortisol, 17α-OHP and nandrolone assays were 0.4 ng/ml, 0.05 ng/ml, and 0.12 ng/ml, respectively, and the analytical recovery ranged from 93.3% to 100%, 94.3% to 98.7%, and 93.5 % to 104%, respectively. In the present study, the strategy adopted for preparing HRP-carbamide and its subsequent use in preparing enzyme conjugate has been shown to result in an increase in thermostability of enzyme conjugate, amino group's availability in enzyme for conjugation with increase in bridge length between analyte and enzyme to reduce steric hindrance.Keywords
HRP-Carbamide, Immunoassay, 17α-OHP, Cortisol, Nandrolone, Urea.- Epimutations and Genetic Aberration Adversely Affect ART Outcome
Authors
1 Department of Anatomy, AIIMS, New Delhi, IN
2 AIIMS, IN
3 ART Center, Army Research and Referral hospital, IN
Source
Journal of Endocrinology and Reproduction, Vol 11, No 1 (2007), Pagination: 49-51Abstract
In the last decade the advent of ART has proven to be a boon for the infertile couple. In a large number of infertile couples there may be a genetic basis. Such couples who harbor genetic abnormalities need to be provided comprehensive counseling prior to opting for ART. Despite state of art technology and professional expertise, the carry home live-birth rate in ART is low. One of the factors responsible for low success rate of ART in couples opting for ART could be genetic. We analyzed 350 couples with recurrent spontaneous abortions (RSA) among whom 86 were couples with recurrent ART/ICSI failure. All couples with 3 or more ART failures were referred for cytogenetic, Yq microdeletion and mitochondrial mutation analysis. Among these 86 couples cytogenetic abnormalities were detected in 32 (20 cases in the male and 12 cases in the female). Yq microdeletions were detected from genomic DNA isolated from blood in 7 men whereas 9 men showed Yq microdeletions in DNA isolated from spermatozoa. High frequency of mitochondrial mutations and deletion of 4977 were also detected in sperm DNA (n=3) and blood of the female partner (n=4). These genetic abnormalities may be iatrogenically transmitted to the offspring. In cases with sex chromosomal and autosomal aberrations there is probability of poor embryo development and, consequently, implantation failure and early fetal loss. ART is a very expensive technique and recurrent ART/IVF failure would result in severe financial burden and physical stress, coupled with emotional stress. It is suggested that all couples opting for ART must undergo genetic analysis.Keywords
ART, Chromosome, Epigenetics, Genetic Counseling, ICSI, Implantation Failure, Recurrent Miscarriage.- To Compare the Effects of Terminalia Arjuna with Rosuvastatin on Total Cholesterol and Low Density Lipoprotein Cholesterol
Authors
1 Department of Pharmacology, Government Medical College Patiala, Punjab, IN
2 Government Medical College Patiala, Punjab, IN
Source
International Journal of Medical and Dental Sciences, Vol 5, No 1 (2016), Pagination: 1056-1066Abstract
Background:In India, CVD is a leading cause of death. Among the modifiable risk factors, hyperlipidemia is one of the important factors. Therefore lowering cholesterol level is a key factor in controlling this disease.Objectives: To compare the effect of Terminalia arjuna, an indigenous drug with Rosuvastatin on serum total cholesterol and low density lipoprotein cholesterol levels, in patients of either sex with dyslipidemia.
Material and Methods: An open prospective randomized controlled study was conducted in on 60 patients for the duration of 12 weeks. Patients were distributed into two groups of 30 patients each. Group I was given Rosuvastatin 10 mg daily and group II was given capsules containing bark powder of T.arjuna 500 mg twice daily. Patients TC and LDL-C levels were performed at baseline and then repeated at 4 weeks, 8 weeks and 12 weeks. The results of both the therapies were then compared and statistically analyzed.
Results: T.arjuna leads to greater reduction in mean TC level than Rosuvastatin (-14.06±8.07% vs -10.10±5.39%), (- 24.73±10.69% vs -19.42±9.98%) and (-27.89±9.25% vs - 24.74±10.02%) at 4, 8 and 12 weeks respectively. The difference between both the groups was statistically non-significant (p>0.05) at 4, 8 and 12 weeks. The reduction in mean LDL-C level was also greater with T.arjuna as compared to Rosuvastatin.
Conclusion: Both Rosuvastatin and T.arjuna were effective in causing significant decrease in serum TC and LDLC levels, but T.arjuna had a slight edge over Rosuvastatin as it showed greater reduction in TC and LDL-C levels as compare to Rosuvastatin. And was found to be safe and well tolerated.
Keywords
Terminalia arjuna, Rosuvastatin, Total Cholesterol, Low Density Lipoprotein, Cholesterol.- Effect of Atorvastatin on E.C.G Changes in Coronary Artery Disease
Authors
1 PGIMER, Chandigarh, IN
2 GMC, Patiala, IN
Source
International Journal of Medical and Dental Sciences, Vol 2, No 1 (2013), Pagination: 34-38Abstract
E.C.G is the most widely used test for both the diagnosing and estimating the prognosis of CAD. ST segment and T wave changes are most commonly evaluated parameters of E.C.G. Atorvastatin have found to decrease ischemic events apart from its hypolipidemic effect. The main objective of this open prospective randomized, controlled study was to evaluate the effect of addition of atorvastatin to conventional antianginal treatment on ST segment and T wave changes on E.C.G. The study was conducted for duration of 2 months in 30 patients of CAD (15 in each group). Group A was started on conventional antianginal treatment and group B was started on Atorvastatin 20 mg once a day at night in addition to conventional treatment. The ECG changes were recorded after 15, 30, 45 and 60 days using 12 lead E.C.G. At the end of the study, no significant difference was found in E.C.G changes between the group A and group B. A slight improvement in ST segment changes in group B patients was observed but the effect was statistically not significant. So, addition of atorvastatin to conventional antianginal treatment did not have a significant effect on reversal of ischemic changes on ECG in patients of CAD.Keywords
CAD, Atorvastatin, E.C.G, ST Segment, T Wave.- Recent and Emerging Trends in Pharmacotherapy of Neuropathic Pain
Authors
1 GMC Patiala, Punjab, IN
2 PGI Chandigarh, IN
Source
International Journal of Medical and Dental Sciences, Vol 2, No 1 (2013), Pagination: 45-58Abstract
Neuropathic pain is a type of chronic pain caused by a lesion or disease of the somatosensory nervous system. The pathophysiology of neuropathic pain is very complex, not fully understood and different from that somatic pain. It has a deleterious effect on health related quality of life, and leads to increased health-care costs and its management is extremely difficult. The response to currently available treatments is less promising, so newer agents with better efficacy and safety are needed. Currently tricyclic antidepressants and anticonvulsants like gabapentin and pregabalin are considered as the 1st line drugs but these are not able to produce complete relief. Various recent drugs are: high dose capsaicin patch, topical lidocaine, botulinum toxin A, lacosamide, Selective Serotinin Reuptake inhibitorss, NMDA antagonists. Certain new targets like endocannabinoid system and various neurotrophic factors like BDNF, NT3, NT4, and GDNF are undergoing preclinical and clinical trials and their role in the treatment of neuropathic pain is still emerging.Keywords
Neuropathic Pain, Nociception, Post Herpetic Neuralgia, Painful Diabetic Neuropathy, Recent Drugs.- Early Eocene Annona Fossils from Vastan Lignite Mine, Surat District, Gujarat, India: Age, Origin and Palaeogeographic Significance
Authors
1 Birbal Sahni Institute of Palaeobotany, 53 University Road, Lucknow 226 007, IN
Source
Current Science, Vol 107, No 10 (2014), Pagination: 1730-1735Abstract
The family Annonaceae has Gondwanan affinity and is being reported from the Cambay Shale of Vastan Lignite Mine on the basis of well-preserved fruit (in counterpart), leaf and pollen grains. This finding is significant because it serves as yet another example of an angiosperm family found in South America and Africa that may have boarded the Indian raft when India was attached to Madagascar, reported on the basis of pollen from Kutch. The Vastan occurrences represent a continuous record from the Indian latest Cretaceous, through the Palaeocene, based on multiple vegetative entities. The well-preserved fruit is morphologically similar to Annona palustris L. At present the dispersal history of the family into India represents an origin in the Lower Cretaceous of North America with later dispersal to South America and Africa and then onto India, as it is recorded from the sedimentary beds associated with the Deccan Volcanics. Another angiosperm family, Dipterocarpaceae, is also found in Vastan, with a similar phytogeographic distribution.Keywords
Annona, Fossil Leaf, Fruit and Pollen, Lignite Mine, Phytogeography.- Clinical Profile of Hepatorenal SyndromeClinical Profile of Hepatorenal Syndrome:A Prospective Study
Authors
1 Ludhiana, IN
2 Punjab Institute of Medical Sciences Jalandhar, Punjab, IN
3 Dayanand medical college and hospital Ludhiana, Punjab, IN
Source
International Journal of Medical and Dental Sciences, Vol 5, No 2 (2016), Pagination: 1241-1246Abstract
Background: Hepatorenal syndrome is the development of renal failure in patients with advanced liver cirrhosis, occasionally fulminant hepatitis, who have portal hypertension and ascitis in the absence of some other kidney disease.
Objective: To study the clinical profile of hepatorenal syndrome.
Methods: All patients of chronic liver disease with renal involvement were studied and patients fulfilling the criteria of hepatorenal syndrome were recruited in the study. The etiology, clinical presentation, morbidity and outcome of patients were recorded. Various variables were studied between survivor group and non survivor group to detect possible predictors of non survival in hepatorenal syndrome. The data was analyzed using SPSS software.
Results: 42 patients of hepatorenal syndrome were clinically evaluated. 95% were males and 5% females with mean age of 50.29±8.87 in survivor group and 45.92±10.1 in non survivor group. High level of serum bilirubin, hepatic encephalopathy, decreased level of albumin, hyponatremia and coagulopathy were significant in non survivor group as compared to survivor group.
Conclusion: The poor prognostic factors were found to be ascites, severe jaundice, hepatic encephalopathy, alcohol abuse, hypoalbuminemia, progressive renal failure and child pugh score greater than 10. Thus hepatorenal syndrome is decompensated cirrhosis which needs judicious treatment especially using terlipressin and albumin.
Keywords
Hepatorenal Syndrome, Profile, Jaundice, Child Pugh Score, Ascitis, Albumin.References
- Arroyo V, Gines P, Jimenez W. Renal dysfunction in cirrhosis. In: Bricher J, Benhamou J, McIntyre N, Rizzetto M, Rodes J, editors. Oxford textbook of clinical hepatology. 2nd ed. England: Oxford university press; 1999.p.733-61.
- Epstein M. Hepatorenal syndrome. Emerging perspectives of pathophysiology and therapy. J Am Soc Nephro 1994;14:1735-53.
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- Salerno F, Gerbes A, Gines P, Wong F, Arroyo V. Diagnosis, prevention and treatment of hepatorenal syndrome in cirrhosis. Gut 2007;56(9):1310–8.
- Lata J. Hepatorenal syndrome. World Journal of Gastroenterology 2012;36(18):4978–84.
- Butt AK, Khan AA, Alam A, Shah SW, Shafqat F, Naqvi AB. Predicting hospital mortality in cirrhotic patients: comparision of child Pugh and acute physiology, age and chronic health evaluation scoring systems. Am J Gastroenterol 1998;93(12):2489-75.
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- Watt K, Uhanova J, Minuk GY. Hepatorenal syndrome: diagnostic accuracy, clinical features and outcome in a tertiary care center. Am J Gastroenterol 2002;97(8):1868-70.
- Colle I, Durand F, Pessione F, Rassiat E, Bernuau J, Barriere E, et al. Clinical course, predictive factors and prognosis in patients with cirrhosis and type 1 hepatorenal syndrome treated with terlipressin: a retrospective analysis. Gastrenterol Hepato 2002;17(18):882-8.
- Moreau R, Durand F, Poynard T. Terlipressin in patients with cirrhosis and type 1 hepatorenal syndrome: a retrospective multicenter study. Gastroenterology 2002;6:2160–1.
- Processing Time Dependent Behaviour of Electrochemical Honing
Authors
1 Department of Mechanical and Industrial Engineering, Indian Institute of Technology, Roorkee, IN
Source
Manufacturing Technology Today, Vol 15, No 5 (2016), Pagination: 31-36Abstract
The aim of the study is to investigate the micro-finish and flatness of the flat functional surfaces made of stainless steel (SS321) using a newly developed electrochemical honing (ECH) machine. ECH is a prominent hybrid machining process (HMP), which is widely used for precision finishing of intricate shapes and hard materials with an efficient and effective manner. In the present work, flat work surface is processed through ECH and pressing time dependent behavior of ECH has been studied. The results show that the ECH of SS321 surfaces gives a glazed appearance and produce an average surface roughness (Ra) of 0.84 μm, and flatness in the range of 5-12 μm with a processing time of 360 seconds. The analysis of finished surface through 3D optical profilometry images has shown improvement in the surface integrity of the workpiece. The microscopic analysis showed that the ECH processed surface is free of burr and scratches.- Effect of Electrolytes on Surface Integrity in Electrochemical Honing Process
Authors
1 Department of Mechanical and Industrial Engineering, Indian Institute of Technology, Roorkee - 247 667, IN
Source
Manufacturing Technology Today, Vol 14, No 10 (2015), Pagination: 14-24Abstract
Electrochemical Honing (ECH) is a process of precision finishing of functional surfaces with the use of the electrical and mechanical energy. It is reported that the 90 percent of the material is removed by electrochemical machining (ECM) process and remaining 10 percent by mechanical scrubbing, which shows the electrical energy is the main constituent in the ECH process. Basically, electrical energy is combined with chemical to form an etching reaction to remove material from the workpiece surface. The electrolyte is pumped through the gap between the tool (cathode) and the workpiece (anode) while a continuous DC current is passed through the cell at a low voltage, so as to dissolve metal from the workpiece. Electrolytes are substances that become ions in solution and acquire the capacity to conduct electricity. The electrolyte has three main functions in the electrochemical machining (ECM) zone. It carries the current between the tool and the work piece, it removes the product of the reaction from the cutting region, and it removes the heat produced by the current flow in the operation. Electrolytes must have high conductivity, low toxicity and corrosivity, and chemical and electrochemical stability. The rate of material removal in ECM is governed by Faraday's laws and is function of current density. Primary variables that affect the current density and MRR are voltage, feed rate, electrolyte conductivity, electrolyte concentration/composition, electrolyte flow rate and material of the work piece. Therefore, electrolyte must be selected carefully for better outcomes.Keywords
ECH, Electrolyte Composition, Electrolyte Concentration, EN52, Ti6Al4V, Surface Finish.- A Comparative Analysis of ECH, PECH and UVECH for Surface Quality Improvement of Bevel Gears
Authors
1 Department of Mechanical and Industrial Engineering, Indian Institute of Technology, Roorkee - 247667, IN
Source
Manufacturing Technology Today, Vol 14, No 7 (2015), Pagination: 15-20Abstract
Surface finishing of bevel gears is an main requirement in various machining shop floors. Variants of electrochemical honing (ECH) could be probable solutions for finishing such complex geometrical parts. This paper presents a comparative analysis of classical ECH, pulse-assisted ECH (PECH) and ultrasonic vibration assisted (UVECH) for tooth flank surface quality enhancement of bevel gears made of EN8 steel. The developed setups and the process has been compared with suitable illustrations. The tooth flank finish was analysed in terms of improvement in surface roughness, morphology of the finished surface and processing time. It was found that the UVECH finished gear exhibited better surface quality and lower processing time as compared to ECH and PECH finished gears. The PECH finished surface also gives better surface quality as compared with ECH, but the use of pulse-on and pulse-off time combination in PECH increases the time required to achieve the desired quality.- Experimental Studies on co-Culture of Shrimp, Litopenaeus vannamei (Boone, 1931) with Sea Cucumber, Holothurian moebii (Ludwig, 1883)
Authors
1 Marine Biological Research Station, Zadgaon, Ratnagiri (M.S.), IN
2 College of Fisheries, Shirgaon, Ratnagiri (M.S.), IN
Source
The Asian Journal of Animal Science, Vol 11, No 2 (2016), Pagination: 81-85Abstract
The pond waste management is a major concern of the shrimp farmers. The sea cucumber,H. moebii (weight: 79.35 ± 0.013 g) with juvenile white leg shrimps, L. vannamei (weight: 2.6 ± 0.18 g) were reared in co-culture and monoculture for 30 days in plastic tank with shrimp pond soil substratum. Shrimp feed was given as per shrimp feeding protocol. Laboratory study suggests that sea-cucumber reducing the percentage organic carbon in the soil. However, growth and survival of shrimp did not differ between monoculture and co-culture. Shrimp increased the level the level of organic carbon in monoculture as compared to co-culture. This study showed that co-culture of two species in earthen ponds appears to be improvement of soil condition of pond.Keywords
Sea Cucumber, Shrimp, Organic Carbon, Soil.- Toxicological Sequelae of Pesticide Combinations Exposure in Buffalo Mesenchymal Stem Cells under In Vitro
Authors
1 Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, IN
2 Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, IN
3 Department of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 29, No 1 (2022), Pagination: 1-14Abstract
The presence of one or more pesticides in a variety of mediums is responsible for their indirect toxicological events leading to cell senescence. In the present investigation, the endeavor was made to see the effect of pesticides Car-Benda-Zim (CBZ) and IMIdacloprid (IMI) alone and in combination with bone marrow-derived Mesenchymal Stem Cells (bMSCs) of buffalo origin. Isolated and cultured bMSCs were exposed to CBZ and IMI alone and in combinations at lower doses. Cells were observed for alterations in cell morphology, oxidative stress, mitochondrial damage and cellular senescence. bMSCs characterized for stem cell surface markers and found to be positive for AP, CD73 and OCT4. bMSCs exposed to IC25, IC12.5 and IC6.25 CBZ and IMI alone and combinations of IC12.5 and IC6.25 of CBZ and IMI. Results revealed significant reduction (p≤0.05) in cell viability noticed on microscopic examination along with loss of normal cell morphology and increased in Reactive Oxygen Species (ROS) positive cells, cells with loss of ΔΨm and number of senescent cells in CBZ and IMI treated groups. Lower dose combination groups showed elevated effects when compared with higher dose alone treated groups and control groups. Present findings suggest that CBZ and IMI induced cytotoxicity in bMSCs mediated via ROS production, altered ΔΨm leading to the cell damage and predisposing senescence process. Moreover, the co-existence of CBZ and IMI in a medium has a considerably more toxic effect than their individual effect.Keywords
Carbendazim, Imidacloprid, Stem Cells, Mitochondrial Transmemberane Potential, Reactive (ROS), SenescenceReferences
- Damalas CA, Koutroubas SD. Farmers’ Training on pesticide use is associated with elevated safety behavior. Toxics. 2017; 5(3):19. https://doi.org/10.3390/toxics5030019. PMID: 29051451; PMCID: PMC5634698.
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