Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Sunkad, Gururaj
- Estimation of Yield Loss in Sunflower Due to New Sunflower Leaf Curl Virus Disease at Different Stages of Crop Growth
Abstract Views :196 |
PDF Views:1
Authors
Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences, RAICHUR (KARNATAKA), IN
2 Department of Plant Pathology, University of Agricultural Sciences, DHARWAD (KARNATAKA), IN
1 Department of Plant Pathology, University of Agricultural Sciences, RAICHUR (KARNATAKA), IN
2 Department of Plant Pathology, University of Agricultural Sciences, DHARWAD (KARNATAKA), IN
Source
International Journal of Plant Protection, Vol 8, No 1 (2015), Pagination: 138-141Abstract
An experiment was carried out to assess the crop loss due to sunflower leaf curl virus (SuLCV) disease. The crop loss assessment in terms of growth and yield components was recorded at first appearance of symptoms of SuLCV at 30 days to 90 days during the crop growth. The SuLCV disease infection in sunflower significantly affected the plant height (72.60 to 157cm), size of the head (8.60 to 18.78cm), 100 seed weight (2.20 to 6.32g), oil content (31.24% to 38.26%), and weight of seeds/10 heads (77.20 to 372.2g) as compared to the healthy control plants. In the plants, first appearance of symptoms at 30 DAS was recorded the seed yield loss of 79.25 per cent.Keywords
Sunflower, SuLCV, Yield Loss Estimation, Yield Components.- Spatial Variability of Mungbean Yellow Mosaic Virus (MYMV) in North Eastern Karnataka
Abstract Views :173 |
PDF Views:0
Authors
Meghashree Meti
1,
Mallikarjun Kenganal
1,
Gururaj Sunkad
1,
D. S. Aswathanarayana
1,
U. K. Shanwad
2
Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences, Raichur (Karnataka), IN
2 Department of Agronomy, University of Agricultural Sciences, Raichur (Karnataka), IN
1 Department of Plant Pathology, University of Agricultural Sciences, Raichur (Karnataka), IN
2 Department of Agronomy, University of Agricultural Sciences, Raichur (Karnataka), IN
Source
International Journal of Plant Protection, Vol 10, No 2 (2017), Pagination: 420-428Abstract
Mungbean a protein rich legume has high demand but, supply is hindered due to poor production and productivity due to mungbean yellow mosaic virus (MYMV) disease. The North Eastern Karnataka being the pulse bowl of the state annually suffers from MYMV incidence. In order to control this whitefly transmitted virus, knowledge and information about its distribution across the region is essential to formulate the strategies of management. In the present study a roving survey was undertaken to know the incidence and present status of MYMV in mungbean among the six districts of North Eastern Karnataka (NEK) region viz., Bellary, Bidar, Koppal, Kalaburgi, Raichur and Yadgir during Kharif 2016, when the crop was at 30 to 45 days old. The GPS position and MYMV incidence in each location were recorded and used to develop GIS map to know the spatial distribution of MYMV in different talukas of six districts. The results showed varied incidence of MYMV across many locations. Highest disease incidence was recorded at Koppal district with 33.33 per cent followed by Bellary (21.45 %), Raichur (19.70 %), Kalaburgi (17.44 %) and Yadgir (15.76 %) districts. The least disease incidence was noticed at Bidar district (5.66%). Higher MYMV incidence in Koppal was mainly due to favourable weather for multiplication and survival of whitefly population which spreads the virus. The virus inoculum in summer crop and weed hosts were found acting as source of inoculum. Findings of the study revealed that higher incidence in Koppal would provide suitable disease pressure for screening of genotypes developed against the MYMV infection and also develop management strategies in each district based on the disease incidences recorded.Keywords
MYMV, Mungbean, Kharif, PDI (% Disease Incidence), Survey, NEK, GPS.References
- Ahmad, M. and Harwood, R. F. (1973). Studies on a whitefly-transmitted yellow mosaic of urdbean (Phaseolus mungo). Pl. Dis., 57: 800-802.
- Bashir, M. (2005). Studies on viral disease of major pulse crops and identification of resistant sources. Tech. Ann. Rep. (April 2004 to June 2005) of ALP., 76 pp.
- Borah, B. and Dasgupta, I. (2012). Begomovirus research in India: A critical appraisal and the way ahead. J. Biosci., 37 (4): 791-806.
- Chenulu, V. V. and Verma, A. (1988). Virus and virus-like diseases of pulse crops commonly grown in India. In: Baldev, B., Ramanujam, S., Jain, H. K. (Eds.), Pulse Crops. Oxford and IBH, New Delhi, pp. 338-370.
- Dhingra, K. L. and Chenulu, V. V. (1985). Effect of yellow mosaic on yield and nodulation of soybean. Indian Phytopathol., 38: 248-251.
- Honda, Y., Iwaki, M. and Saito, Y. (1983). Mechanical transmission, purification and some properties of whitefly-borne mungbean yellow mosaic virus in Thiland. Pl. Dis., 67: 801-804.
- Hull, R. (2004). Mathew’s plant virology. 4th Ed. Elsevier Publishers, India, pp.180-182.
- Jeske, H. (2009). Gemini viruses. Curr Top Microbiol. Immunol., 331: 185-226.
- Jones, D.R. (2003). Plant viruses transmitted by whiteflies. European J. Pl. Pathol., 4 (1): 195-219.
- Khan, M. A., Rashid, A. and Mateen, A. (2012). Incidence of mungbean yellow mosaic virus, its epidemiology and management through Mycotal, Imidachloprid and Tracer. Agric. & Bio. J. N. America, 3 (11) : 476-480.
- Malathi, V. G., Surendranath, B., Naghma, A. and Roy, A. (2005). Adaptation to new hosts shown by the cloned components of mungbean yellow mosaic India virus causing cowpea golden mosaic in northern India. Can. J. Plant Pathol., 27 : 439-447.
- Malik, B. A. and Bashir, M. (1992). Major diseases of food legume crops of Islamic countries. In: Jamil, F. F., Naqvi, S.H.M. (Eds.), Proceedings of COMSTECH-NAIB International Workshop of Agroclimatology Pests and Disease and Their Control. pp. 25-38.
- Manjunath, B., Jayaram, N., Muniyappa, V. and Prameela, H. A. (2013). Status of yellow mosaic virus and whitefly Bemesia tabaci biotypes on mungbean in Southern Karnataka. Department of Plant Pathology. Bangalore. Leg. Res., 36 (1): 62 - 66.
- Mansoor, S., Briddon, R.W., Zafar, Y. and Stanley, J. (2003). Gemini virus disease complexes: an emerging threat. Trends Plant Sci., 8:128-134.
- Murugesan, S. and Chelliah, S. (1977). Influence of sowing time on the incidence of the vector Bemisia tabaci (Genn.) and the yellow mosaic disease of greengram. Madras Agril. J., 64 (2): 128-130.
- Nath, P. D. and Saikia, A. K. (1995). Effect of time of sowing on the incidence of mungbean yellow mosaic virus disease and whitefly (Bemisia tabaci Genn.) population in green gram. Anns. Agric. Res., 16 (4): 483-484.
- Nene, Y. L. (1973). Viral diseases of some warm weather pulse crops in India; Plant Dis. Rep., 57: 463-467.
- Panduranga, G. S., Reddy, P. K. and Rajashekar, H. (2012). Survey for incidence of mungbean yellow mosaic virus (MYMV) in mungbean [Vigna radiata (L.) Wilczek]. Environ. & Ecol., 30 (3): 1030-1033.
- Qazi, J., Ilyas, M., Manseor, S. and Briddan, R.W. (2007). Legume yellow mosaic virus: genetically isolated begomovirus mole. Plant Patholo., 8 (4) : 343-348.
- Rosen, R., Kanakala, S., Kliot, A., Pakkianathan, B.C. , Farich, B.A. and Ghanim, M. (2015). Persistant, circulative transmission of begomoviruses by whitefly vectors. Curr. Opn Virol., 15: 1-8.
- Salam, S.A., Patil, M. S. and Byadgi, A.S. (2011). Satus of mungbean yellow mosaic virus disease incidence on greengram. Karnataka J. Agric. Sci., 24 (2): 247-248.
- Singh, D.P. (1991). Genetics and breeding of pulse crops. Kalyani Publ, Ludhiana, India, pp. 6-11.
- Singh, R.A. and Gurha, S.N. (1994). Influence of cropping seasons on the incidence of yellow mosaic disease in mungbean genotypes. Indian J. Pulse Res., 7 (12): 206-208.
- Usharani, K. S., Surendranath, B., Haq, Q. M. R. and Malathi V. G. (2004). Yellow mosaic virus infecting soybean in Northern India is distinct from the species infecting soybean in southern and western India. Curr. Sci., 86:845-850.
- Varma, A., Dhar, A. K. and Mandal, B. (1992). MYMV transmission and control in India. In: Green SK, Kim D, editors. Mungbean yellow mosaic disease. Taipei: Asian Vegetable Research and Development Centre; p. 8-27.
- Anonymos (2016). www. Indiastat. com. (2015-2016) Ministry of Agriculture and Farmers welfare, Govt. of India.
- Standardization of Inoculation Technique to Identify the Sources of Resistance against Stem and Pod Rot of Groundnut
Abstract Views :423 |
PDF Views:0
Authors
Poornima
1,
Gururaj Sunkad
2
Affiliations
1 Department of Plant Pathology, AICRP (Sunflower), MARS, Raichur (Karnataka), IN
2 Department of Plant Pathology, University of Agricultural Sciences, Raichur (Karnataka), IN
1 Department of Plant Pathology, AICRP (Sunflower), MARS, Raichur (Karnataka), IN
2 Department of Plant Pathology, University of Agricultural Sciences, Raichur (Karnataka), IN
Source
International Journal of Plant Protection, Vol 13, No 1 (2020), Pagination: 58-61Abstract
A technique was standardized to screen for resistance to stem rot (Sclerotium rolfsii Sacc.) in groundnut (Arachis hypogaea L.). A total of seven different inoculation techniques were screened by using susceptible genotype TMV-2. The techniques involved inoculation of 10 day old groundnut plants raised in pots by spreading mycelial propagules of S. rolfsii grown on sorghum grain medium (SGM) on soil surface, Inoculum spread on the soil surface and covered with groundnut leaf debris, Inoculum placed around the collar region, Inoculum placed around the collar region and covered with groundnut leaf debris, Inoculum mixed in the soil, Inoculum mixed in the soil and covered with groundnut leaf debris, Agar disc method. Among these techniques inoculum spread on the soil surface and covered with groundnut leaf debris was found to be most efficient in getting highest per cent incidence of stem rot (84.86%) and pod rot (70.48%).Keywords
Groundnut, Stem, Pod Rot, S.rolfsii.References
- Anonymous (2014). Status paper on oilseeds, National Mission on Oilseeds and Oil Palm (NMOOP), New Delhi. pp. 24-25.
- Beute, M.K. and Kabana, R.R. (1979a).Effect of volatile compounds form remoistened plant tissues on growth and germination of sclerotia of Sclerotium rolfsii. Phytopathol., 69 : 802-805.
- Beute, M.K. and Kabana, R.R. (1979b).Effect of wetting and the presence of groundnut tissues on germination of sclerotia of Sclerotium rolfsii produced in soil. Phytopathol., 69: 869-872.
- Ghewande, M.P., Desai, S. and Basu, M.S. (2002).Diagnosis and management of major diseases of groundnut, N.R.C.G., Junagadh. p. 11.
- Pande, S., Rao, J.N., Reddy, M.V. and Mcdonald, D. (1994). Development of a greenhouse screening technique for stem rot resistance in groundnut. Internat. Arachis Newsltr., 14: 23-24.
- Patil, M.B., Patil, G.D and Wani, P.V. (1977).Varietal reaction of groundnut against Sclerotium rolfsii. Indian phytopath 30: 562.
- Patil, M.B. and Rane, M.S. (1983).Studies on host range effect of plant age on susceptibility and varietal reaction of groundnut to Sclerotium rolfsii. Indian J. Mycol. Pl. Pathol., 13(2): 183-186.
- Sennoi, R., Jogloy, S., Saksirirat, W., Kesmala, T., Singkham, N. and Patanothai, A. (2012).Levels of Sclerotium rolfsii inoculum influence identification of resistant genotypes in Jerusalem artichoke. African. J. Microbiol. Res., 6(38): 6755-6760.
- Shew, B.B., Wynne, J.C. and Beute, M.K. (1987).Field microplot and greenhouse evaluation for resistance to Sclerotium rolfsii in peanut. Pl. Dis., 71 : 188-191.
- Shokes, F.M., Rozalski, K., Gorbet, D.W., Brenneman, T.B. and Berger, D.A. (1996).Techniques for inoculation of peanut with Sclerotium rolfsiiin the greenhouse and field. Peanut Sci., 23: 124-128.
- Antagonistic potential and growth promoting activities of novel indigenous strains of Trichoderma
Abstract Views :103 |
PDF Views:68
Authors
Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences, Raichur-584104, India ., IN
2 Department of Plant Pathology, University of Agricultural Sciences, Raichur-584104, India., IN
1 Department of Plant Pathology, University of Agricultural Sciences, Raichur-584104, India ., IN
2 Department of Plant Pathology, University of Agricultural Sciences, Raichur-584104, India., IN
Source
Journal of Biological Control, Vol 36, No 2 & 3 (2022), Pagination: 112 - 119Abstract
Rice (Oryza sativa L.), as a cereal grain, is the most widely consumed staple food for a large part of the world’s human population, especially in Asia and Africa. Rice production is often subjected to several biotic and abiotic stresses. Rice sheath blight is one of the most destructive diseases causing economic losses in rice yields and affecting quality worldwide. Twenty soil samples were collected from the rhizosphere of rice crop from different regions of Northern Karnataka. Trichoderma spp. were isolated from the rhizospheric soil samples. The antagonistic potential of Trichoderma spp. was studied using dual culture technique. Among twenty strains of Trichoderma the highest inhibition (>50%) was recorded in nine strains which ranged from 54.20 to 65.10 per cent. Trichoderma harzianum recorded highest seed germination (100%), root length (13.73 cm), shoot length (8.64 cm) and seedling vigour index. In pot culture experiment, the Trichoderma strains significantly improved the growth parameters such as root length, shoot length and number of tillers per plant. Among the different treatments, seed treatment + root dipping + foliar spray with T. harzianum strain was highly effective in increasing the shoot length, root length and seedling vigour index at 30, 60 and 90 days after sowing. This strain was found highly effective in inhibiting the pathogen and promoting the growth of rice plants.Keywords
Plant growth promotion, rice, sheath blight, Trichoderma strainsReferences
- Baki AAA, Anderson JD. 1973.Vigour determination in soybean seed by multiple criteria. Crop Sci, 13: 630-633. https://doi.org/10.2135/cropsci1973.0011183X00 1300060013x
- Broadbent P, Baker KF, Water-worth Y. 1971. Bacteria and action mycetes antagonistic to fungal root pathogens in Australian soils. Aust J Biol Sci, 24: 925-944. https:// doi.org/10.1071/BI9710925 PMid:5003563
- Das BC, Bhuyan SA, Bora LC. 1995. Comparative efficacy of Trichoderma viride in suppressing sheath blight of rice by different methods of application. Plant Health. 1: 7-11.
- Das BC, Khairuzzaman ASM, Bora LC. 1998. Biological seed treatment for management of sheath blight of rice. J Mycol Plant Pathol, 28: 45-47.
- International Seed Testing Association. 1993. International rules for seed testing. Seed Sci Technol, 24.
- Kumari A, Kumar R, Maurya S, Pandey PK. 2016. Antagonistic potential of different isolates of Trichoderma against Rhizoctonia solani. Europena J Expt Biol, 6(2): 22489215.
- Panse VG, Sukathme PV. 1985. Statistical methods for agricultural workers. ICAR Publications, New Delhi, pp. 145-155.
- Patil RK, Goyal SN, Patel BA, Patel RG, Patel DJ, Singh RV, Pankaj, Dhawn SC, Gour SH. 2003. Integrated management of stalk rot disease and phyto-nematodes in Rabi maize. Proceedings of National Symposium on Biodiversity and Management of Nematodes in Cropping Systems for Sustainable Agriculture, Jaipur, pp. 250-254.
- Ravi S, Valluvaparidasan V, Jeyalakshmi C, Doraiswamy S. 1999. Effect of biocontrol agents on seed borne Colletotrichumin French bean. Plant Dis Res, 14(2): 146-151.
- Roy AK. 1979. Susceptibility of rice plants to sheath blight at different stages of growth. KAVAKA. 7: 25-26.
- Seema M, Devaki NS. 2012. In vitro evaluation of biological control agents against Rhizoctonia solani. J Agric Technol, 8: 233-40.
- Sharma KK, Singh US. 2014. Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma. J Nat Appl Sci, 6(2): 451-456.https://doi.org/10.31018/jans.v6i2.481
- Sunder S, Singh R, Dodan DS. 2003. Standardization of inoculation methods and management of sheath blight of rice. Indian J Plant Pathol, 21: 92-96.
- Vidhyasekaran P, Rabindran R, Muthamilan M, Nayar K, Rajappan K, Subramanian N,Vasumathi K. 1997.
- Development of powder formulation of Pseudomonas fluorescens for control of rice blast. Plant Pathol, 46:
- -297. https://doi.org/10.1046/j.1365-3059.1997.d01-27.x
- Zheng Z, Shetty K. 2000. Enhancement of pea (Pisum sativum) seedling vigour and associated phenolic
- content by extracts of apple pomace fermented with Trichoderma spp. Process Biochem, 36: 79-84. https://
- doi.org/10.1016/S0032-9592(00)00183-7 .
- Exploitation of indigenous fluorescent pseudomonads against stem and pod rot of groundnut caused by Sclerotium rolfsii
Abstract Views :116 |
PDF Views:79
Authors
Affiliations
1 Department of Pathology, University of Agricultural Sciences, Raichur - 584104, Karnataka, India ., IN
1 Department of Pathology, University of Agricultural Sciences, Raichur - 584104, Karnataka, India ., IN
Source
Journal of Biological Control, Vol 36, No 2 & 3 (2022), Pagination: 120 - 129Abstract
Stem and pod rot of groundnut is an economically important soil borne disease caused by Sclerotium rolfsii Sacc. The present study aimed to evaluate the indigenous bacterial bio control agent Pseudomonas fluorescens strains having multiple traits related to bio control and plant growth promoting activity. Healthy rhizospheric soil from groundnut field was collected from different North Karnataka districts. Nineteen strains of P. fluorescens were isolated by serial dilution technique on King’s B medium. The antagonistic potential of nineteen strains was tested against S. rolfsii by using dual culture technique. The per cent inhibition was highly variable among the nineteen strains of P. fluorescens ranging from 2.59 to 75.18 per cent. Maximum mycelial inhibition of S. rolfsii was recorded by the strain PF-2 (75.18%) followed by PF-3 (72.96%), PF-6 (69.62%) and least inhibition was recorded by PF-13 (2.59%). Five superior strains of P. fluorescens showed fluorescens under UV light, yellowish green pigmentation, rod shaped cells under microscope. Five superior strains were subjected for various biochemical tests and all the isolates were positive for biochemical characterization such as Gram staining, endospore production, catalase, starch hydrolysis, urease test, casein hydrolysis and gelatin liquefy action and negative for KOH and in dole test. Further, these strains were subjected for plant growth promoting traits such as HCN production, IAA production, siderophore production and volatile compounds production. Among these, the strainsviz., PF-2 and PF-3 were scored as strong with respect to antagonism and growth promotion. The strainsPF-6, PF-7 and PF-10 were scored as moderate with light brown colour. Among the tested strains of P. fluorescens, the isolates PF-2 and PF-3 were recorded higher production of siderophore, isolates PF-6, PF-7 and PF-10 showed moderate production of siderophore. The strain PF-2 of P. fluorescens showed highest per cent of mycelial inhibition of S. rolfsii indicating higher production of volatile compounds, followed by PF-3 and PF-6. Whereas, the least mycelial inhibition was recorded by the isolate PF-10 indicating less production of volatile compounds.Keywords
Groundnut, management, Pseudomonas, sclerotium rotReferences
- Anonymous, Department of economics and statistics, GOI.2022, Annual report (2021- 22).
- Blazevic DJ, Ederer GM. 1975. Principles of biochemical tests in diagnostic microbiology. New York: Wiley and Company, 5: 13-45.
- Cappuccino JC, Sherman N. 1992. In: Microbiology. A Laboratory Manual, NewYork. pp. 125-179.
- Cartwright DK, Benson DM. 1985. Biological control of rhizoctonia stem rot of poinsettia in polyfoam rooting cubes with Pseudomonas cepacia and Paecilomyces lilacinus. Biol Control, 5(2): 237-244. https://doi.
- org/10.1006/bcon.1995.1029
- Charulatha R, Harikrishnan H, Manoharan PT, Shanmugaia V. 2013. Characterization of groundnut rhizosphere Pseudomonas sp. VSMKU 2013 for Control of Phytopathogens. Microbiol Res Agroeco Manage. 121127. https://doi.org/10.1007/978-81-322-1087-0_8
- Cook RJ, Baker KF. 1983. The nature and practice of biological control of plantpathogens. American Phytopathol Soc St Pau Minnesota. pp. 539.
- Eckford MQ. 1927. Thermophile bacteria in milk. American J Hygiene, 7: 200-202. https://doi.org/10.1093/ oxfordjournals.aje.a120412
- Grichar VJ, Bosweel TE. 1987. Comparison of lorsban and tilt with terrachlor for control of southern blight on peanut the texas. Agriculture Experiment Station. pp. 4534.
- Mallesh SB, Lingaraju S, Benagi VI, Basamma K, Kumari, Vinaya, H. 2009. Native plant promoting rhizobacteria for the suppression of pathogens of pomegranate (Punica granatum). 2nd International Symposium on Pomegranate and Minor including Mediterranean fruits (ISPMMF-2009), UAS, Dharwad, India.
- Mayee CD, Datar VV. 1988. Diseases of groundnut in the tropics. Rev Trop Plant Pathol. 5: 85-118.
- Neilands JB. and Schwayn A. 1987. Siderophores in relation to plant growth and disease. Ann. Rev Pl Physiol. 37: 187-208.
- Nirmala JL, Reddy ES. 2014. Evaluation of plant growth promoting attributes and biocontrol potential of native fluorescent Pseudomonas spp. against Aspergillus niger causing collar rot of ground nut. Inter J Plant An Env Sci, 4(4): 256-262.
- Priyanka, Geeta, Goudar P, Nath JN, Patil PV. 2017.Isolation, characterization and antagonistic activity of fluorescent pseudomonads. Int J Curr Microbiol Appl Sci, 6(12): 3883-3898. https://doi.org/10.20546/ ijcmas.2017.612.449
- Rakh RR, Raut LS, Dalvi AV, Manwar AV. 2011. Biological control of Sclerotium rolfsii, causing stem rot of groundnut by Pseudomonas monteilii. Recent Res Sci Technol, 3(3): 26-34
- Roopa KP,Krishnaraj, PU. 2017. Effect of Actinobacteria and Pseudomonas spp. against Sclerotium rolfsii in groundnut. Int J Curr Microbiol Appl Sci, 6(10): 229-245.https://doi.org/10.20546/ijcmas.2017.610.029
- Sallam EK, Pervaiz A, Abbasi. 2013. Isolation, characterization, and formulation of antagonistic bacteria for the management of seedlings damping off and root rotdisease of cucumber. Canadian J Microbiol, 60(1): 25-33. https://doi.org/10.1139/cjm-2013-0675 PMid:24392923
- Saravanan T, Muthuswamy M, Marimuthu T. 2013. Development of integrated approach manage the
- fusarium wilt of banana. Crop Protect, 22(9): 1117- 1123. https://doi.org/10.1016/S0261-2194(03)00146-7
- Seeley HW, Vandermark PJ. 1970. Microbe in Action, A laboratory manual of microbiology, DB Taraporvala
- Sons and Company Pvt, Ltd, Mumbai. pp. 86-95.
- Shreedevi S. 2017. Investigations on major soil borne diseases of groundnut with special emphasis on dry root rot caused by Rhizoctonia bataticola (Taub.) Butler. MSc. Thesis, Univ Agril Sci, Raichur, Karnataka. pp. 52-54.
- Shruthi TH. 2017. Ecofriendly management of wilt of pomegranate caused by Cerotocystis fimbriata Ellis and Halst. through bioagents. MSc Thesis, Univ Agril Sci, Raichur, Karnataka. pp. 55.
- Indigenous Bacterial Endophytic Pgpms of Chickpea: Characterization and Hidden Antagonistic Potential Against Rhizoctonia Bataticola Causing Dry Root Rot of Chickpea
Abstract Views :150 |
PDF Views:80
Authors
Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences, Raichur – 584104, Karnataka, IN
1 Department of Plant Pathology, University of Agricultural Sciences, Raichur – 584104, Karnataka, IN
Source
Journal of Biological Control, Vol 36, No 4 (2022), Pagination: 222-233Abstract
Chickpea (Cicer arietinum L.) is grown in more than 50 countries. India is the largest chickpea-producing country accounting for 64% of the global chickpea production. However, the production is contrained by the dry root rot disease caused by Rhizoctonia bataticola. Considering this problem, the investigation was carried out to isolate, characterize and the antagonistic potential of indigenous endophytic PGPMs for one of the components in the integrated management of dry root rot of chickpeas in eco-friendly manner. Hence, the isolation of thirty endophytic PGPMs was carried from chickpea by using the spread plate technique. The cultural characters and Gram’s staining reaction confirmed that the endophytic PGPMs isolated from chickpea plant tissues were bacteria. Among thirty bacterial strains, eight showed more than 50% of mycelial inhibition of the pathogen. Out of eight strains, five highly superior strains were selected and subjected for 16S rDNA gene sequencing using the universal primers (16Sr DNA F and 16Sr DNA R), which produced amplified products of size 1500 bp. nBLAST results of 16S rDNA gene sequence revealed that all the endophytic bacterial PGPMs showed homology with genus Bacillus but with different species. The five potential strains namely, BEPGPM-5, BEPGPM-9, BEPGPM-27, BEPGPM-28, and BEPGPM-30 were identified and confirmed as B. tropicus, B. pacificus, B. cereus, B. subtilis, respectively, based on molecular technique.Keywords
Bacillus cereus, B. pacificus, B. subtilis, B. tropicus, chickpea, dry root rotReferences
- Bhavani D, Anday M, and Kumar K. 2015. Chickpea endophytic bacteria inhibiting dry root rot fungus Rhizoctonia bataticola. Int. J Sci Eng Res, 6(2): 83–85.
- Chiranjeevi N, Kumar MR, Padmodaya B, Venkateswarlu NC, Sudhakar P, Jayalakshmi Devi RS, and Jyothsna MK. 2020. In vitro evaluation of endophytic bacteria for their efficacy against chickpea dry root rot causing pathogen (Rhizoctonia bataticola (Taub.) Butler. Int J Curr Microbiol Appl Sci, 9(12): 2028–2043. https://doi.org/10.20546/ijcmas.2020.912.240
- Etesami H. 2020. Plant microbe interactions in plants and stress tolerance. Elsevier. 102–112. https://doi.org/10.1016/B978-0-12-818204-8.00018-7
- Hadimani B. 2018. Studies on isolation of endophytes and their efficacy against soil borne fungal pathogens in tomato. PhD, Thesis. Univ. Agic. Sci., Dharwad, Karnataka (India).
- Khan RA, Bhat TA and Kumar K. 2013. Screening of chickpea (Cicer arietinum L.) germplasm lines against dry root rot caused by Rhizoctonia bataticola (taub.) Butler. Asian J Pharm Clin Res. 6: 211–212.
- Lopes MJS, Dias-Filho MB and Gurgel ESC. 2021. Successful plant growth-promoting microbes: Inoculation methods and abiotic factors. Front Sustain Food Syst., 5: 200–216. https://doi.org/10.3389/fsufs.2021.606454
- Mitra D, Anđelkovic S, Panneerselvam P, Manisha SA, Vasic T, Ganeshamurthy AN, Verma D, Poonam Radha TK and Divya J. 2019. Plant Growth Promoting Microorganisms (PGPMs) helping in sustainable agriculture: Current perspective. Int J Agril Sci Vet Med., S(2): 50–74.
- Salo EN and Novero A. 2021. Identification and characterisation of endophytic bacteria from coconut (Cocos nucifera) tissue culture. Tropical Life Sci Res., 31(1): 57–68. PMid: 32963711 PMCid: PMC7485530. https://doi.org/10.21315/tlsr2020.31.1.4
- Schaad NW. 1992. Xanthomonas. In: Laboratory guide for identification of plant pathogenic bacteria, II Ed. International Book Distributing Co. Lucknow. p. 165.
- Sharma M and Pande S. 2013. Unravelling effects of temperature and soil moisture stress response on development of dry root rot [Rhizoctonia bataticola (Taub.)] Butler in chickpea. American J Plant Sci., 4: 584–589. https://doi.org/10.4236/ajps.2013.43076
- Vincent JM. 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nat. 150: 850. PMid: 20343980. https://doi.org/10.1038/159850b0
- Xu SJ and Kim BS. 2014.Biocontrol of Fusarium crown and root rot and promotion of growth of tomato by Paenibacillus strains isolated from soil. Mycobiol., 42: 158–166. PMid: 25071385 PMCid: PMC4112232. https://doi.org/10.5941/MYCO.2014.42.2.158