Informatics Publishing Limited

Mukund Nagarnaik ¹, Arun Sarjoshi ¹, D. G. Kshirsagar ¹, Pranjali Linge ¹, Shital Bhore ¹, Zeba Qureshi ¹, Girish Pandya ¹

Affiliations
1 Research and Development Division, Qualichem Laboratories Pvt. Ltd, 4,North Bazar Road, Gokulpeth Market, Nagpur 440010, IN

Abstract

Ayurveda is a traditional medicine used worldwide, consisting principally of herbs and regulated under various legislation in each country. The Ayurvedic products are perceived as safe by consumer because of natural ingredients, but their use is not free from risks. This study aimed to assess the microbiological contamination of some herbal products commonly used in Ayurvedic medicine, produced in India. About 20 Ayurvedic drugs bought from different Indian shops in the form of powder, tablets, capsules, syrup, cream were analysed for total aerobic bacterial count as well as total fungi in the form of yeast and mould..Some pathogens like E. coli has also been estimated. The Ayurvedic products tested are of some concern for consumer, especially those sold in herbalist's shops and street markets. The risk seem to be not related to the original plant, but probably to contamination of the products by bacteria and fungi and possibly by chemical substances derived from the production process. Products of industrial origin seem to be substantially safe, suggesting that complying with standard controls procedures during production and distribution process leads to better product quality and safety. In this study attempt is also made to in estimating measurement uncertainty for microbiological analysis of drug samples. Customers are increasingly demanding from microbiological labs to provide MU results along with routine analysis of their products .This could serve for al those engaged in similar analysis work. Microbiological results suggest that the products of industrial origin, bought from city drugstores, were less contaminated than those from local production available in street markets.

Keywords

Microbial Analysis, Herbal Medicines, Ayurvedic, Uncertainty.

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Mukund Nagarnaik ¹, Arun Sarjoshi ¹, Pranjali Linge ¹, Shital Bhore ¹, Girish Pandya ²

Affiliations
1 Qualichem Laboratories Pvt. Ltd., 4, North Bazar Road, Gokulpeth Market, Nagpur 440010, IN
2 Research and Development Division, Qualichem Laboratories Pvt. Ltd., 4, North Bazar Road, Gokulpeth Market, Nagpur 440010, IN

Abstract

Personal care products contain variable amounts of natural material such as nutrients that support microbial growth. Cosmetic products such as shampoo, hand and body lotion, facial wash, and liquid soaps were obtained from the local market and analyzed for their microbial content. In this study out of forty cosmetic products analyzed, twenty were found to contain yeast and mould and gave total bacterial count. However these were within the limits of BIS 14648. No pathogens were found in the samples. Some raw materials such as xanthan gum and veegum ultra clay were also analysed for microbial content.

Keywords

Microbial Analysis, Cosmetics, Xanthan Gum, Veegum Ultra.

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Mukund Nagarnaik ¹, Anil Dhakulkar ¹, Arun Sarjoshi ¹, Girish Pandya ¹

Affiliations
1 Research and Development Division, Qualichem Laboratories Pvt. Ltd., 4,North Bazar Road, Gokulpeth Market, Nagpur 440010, IN

Abstract

A reverse phase HPLC method was developed for quantitative estimation of nicotine in nicotine polacrilex chewing gums excessively used in nicotine replacement therapy (NRT) adopted to replace the nicotine from cigarettes used by smokers. Further the method was validated to ensure the feasibility of the method for its application in routine analysis. Parameters such as specificity, linearity, quantitation limits, precision, and accuracy were determined. The minimum detection limit was 0.25 μg/ml and LOQ was 0.75 μg/ml. Studies were also carried out to estimate the uncertainty associated with the analytical method by applying a bottom-up approach. All data appearing in this study complies with NABL 17025 requirements. It was implemented in our laboratory as a routine method and our laboratory was accredited. The uncertainty in each step was estimated.. The expanded uncertainty in the estimation of nicotine was ±0.06

Keywords

Nicotine Gum, RP-HPLC, Validation, Uncertainty.

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