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Jadhav, Ghanshyam B.
- Analgesic and Antiinflammatory Activity of Amarwel Extracts on Experimentally Induce Pain and Inflammation on Animals
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Materials and Methods: Petroleum ether extract, methanol extract, and aqueous extracts of Cuscuta reflexa (PECR, MECR, and AECR respectively) at three dose levels of 50, 200 and300 mg/kg body weight of an animal by oral route were used for biological activities. The analgesic activity of was determined using hot plate analgesia, acetic acidinduced writhing response and formalin test. The anti-inflammatory activity of was determined using models like carrageenan, serotonin and histamine-induced paw edema models along with cotton pellet induced granuloma. Probable mechanism involved in the antiinflammatory effect of methanol and aqueous extracts was evaluated with various tests like ulcerogenicity test; acetic acid induced vascular permeability test, and leukocyte migration test using a single dose of 300 mg/kg orally.
Results: The HPTLC analysis showed presence of quercetin in MECR and AECR (0.121 and 0.071 mg% respectively). Acute oral toxicity test revealed the LD50 of >2 g/kg. PECR, MECR and AECR (200 and 300 mg/kg p.o.) significantly (P<0.05) increased latency against thermal stimulus, decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, MECR and AECR (200 and 300 mg/kg p.o.) exhibited significant (P<0.01) antiinflammatory effect against carrageenan and mediator-induced paw edema.
Conclusion: Results suggest significant analgesic and anti-inflammatory effects produced by MECR and AECR.
Authors
Affiliations
1 KCTS R. G. Sapkal College of Pharmacy, A/p-Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, Maharashtra, IN
2 Bhujbal Knowledge City, MET's Institute of Pharmacy, Adgaon, Nashik, Maharashtra, IN
3 KCT'S RGS College of Pharmacy, Anjaneri, Nashik, Maharashtra, IN
4 SNJB's SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra, IN
1 KCTS R. G. Sapkal College of Pharmacy, A/p-Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, Maharashtra, IN
2 Bhujbal Knowledge City, MET's Institute of Pharmacy, Adgaon, Nashik, Maharashtra, IN
3 KCT'S RGS College of Pharmacy, Anjaneri, Nashik, Maharashtra, IN
4 SNJB's SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 6, No 2 (2014), Pagination: 112-117Abstract
Objective: To study Analgesic and anti-inflammatory activity of Amarwel extracts on experimentally induce pain and inflammation on animalsMaterials and Methods: Petroleum ether extract, methanol extract, and aqueous extracts of Cuscuta reflexa (PECR, MECR, and AECR respectively) at three dose levels of 50, 200 and300 mg/kg body weight of an animal by oral route were used for biological activities. The analgesic activity of was determined using hot plate analgesia, acetic acidinduced writhing response and formalin test. The anti-inflammatory activity of was determined using models like carrageenan, serotonin and histamine-induced paw edema models along with cotton pellet induced granuloma. Probable mechanism involved in the antiinflammatory effect of methanol and aqueous extracts was evaluated with various tests like ulcerogenicity test; acetic acid induced vascular permeability test, and leukocyte migration test using a single dose of 300 mg/kg orally.
Results: The HPTLC analysis showed presence of quercetin in MECR and AECR (0.121 and 0.071 mg% respectively). Acute oral toxicity test revealed the LD50 of >2 g/kg. PECR, MECR and AECR (200 and 300 mg/kg p.o.) significantly (P<0.05) increased latency against thermal stimulus, decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, MECR and AECR (200 and 300 mg/kg p.o.) exhibited significant (P<0.01) antiinflammatory effect against carrageenan and mediator-induced paw edema.
Conclusion: Results suggest significant analgesic and anti-inflammatory effects produced by MECR and AECR.
Keywords
Dodder, Hot Plate, Writhing, Formalin Test, Carrageenan, Granuloma, Quercetin.
References
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- Free Radical Scavenging and Antioxidant Activity of Punica Granatum Linn
Abstract Views :250 |
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Authors
Affiliations
1 KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, University of Pune, Maharashtra, IN
1 KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist-Nashik, University of Pune, Maharashtra, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 4, No 2 (2014), Pagination: 51-54Abstract
Free radical scavenging activity of Punica granatum Linn. was carried out by DPPH (1, 1-diphenyl-2-picryl hydrazyl) method, Reducing power assay, Scavenging activity against hydrogen peroxide, Total antioxidant activity, Estimation of total flavonoid content in vitro methods. Punica granatum Linn (PG) is rich source of two types of polyphenolic compounds: anthocynins such as delphidin, cyanidin, and pelargonidin which give the fruit and the juice its red colour; and hydrolysable tannins such as punicalin, pedunculagin, gallic and ellagic acid esters of glucose which accounts for 92% of the antioxidant activity of the whole fruit. Oxidative stress (ost) depicts the existence of products called free radicals (molecules possessing an unpaired electron) and reactive oxygen species (ros), which are formed, in normal physiology but become deleterious when not being quenched by a cascade of antioxidants systems (aos). Antioxidant plays an important role in inhibiting and scavenging free radicals thus providing protection to human against infections and degenerative diseases. In the present study Punica granatum Linn found the excellent antioxidant activity as measured by various methods such as DPPH radical scavenging activity, reducing power assay, H2O2 scavenging activity and total antioxidant activity.Keywords
Antioxidant, DPPH, Flavonoids, Oxidative Stress.References
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- Spectrophotometric Determination of and Validation of Zidovudine Concentration in Bulk and Dosage Form
Abstract Views :223 |
PDF Views:2
Authors
Affiliations
1 KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist- Nashik, Maharashtra, IN
2 Dept. of Pharmaceutical Chemistry, M.V.P's College of Pharmacy, Gangapur Road, Nashik, Maharashtra, IN
1 KCT’s R. G. Sapkal College of Pharmacy, Anjaneri, Tal-Trimbakeshwar, Dist- Nashik, Maharashtra, IN
2 Dept. of Pharmaceutical Chemistry, M.V.P's College of Pharmacy, Gangapur Road, Nashik, Maharashtra, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 4, No 2 (2014), Pagination: 51-53Abstract
A simple, accurate, precise, sensitive and a highly selective ultra violet spectrophotometric method has been developed for the simultaneous estimation of in bulk and solid dosage form. The estimation of Zidovudine was carried out at 266 nm. The developed method was validated for linearity, range, precision, recovery studies and interference study for mixture. All these parameters showed the adaptability of the method for the quality control analysis of the drug in bulk and in marketed formulations.Keywords
Zidovudine, UV- Method, Spectrometric Method, Determination.- Synthesis of Novel Protein Tyrosine Phosphatases 1b inhibitors 2, 5-Disubstituted Oxadiazole
Abstract Views :187 |
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Authors
Affiliations
1 KCT’S RGS College of Pharmacy, Anjaneri, Nashik, Maharashtra, IN
2 SNJB’s SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra, IN
1 KCT’S RGS College of Pharmacy, Anjaneri, Nashik, Maharashtra, IN
2 SNJB’s SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra, IN