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G. Kumaraswamy ¹, Repudi Lalitha ¹, D. Sudheer Kumar ¹

Affiliations
1 Department of Pharmaceutical Analysis, Care College of Pharmacy, Athamakur (Mdl), Oglapur(V), Warangal (Dist), Telangana, IN

Abstract

The present work was aimed at method development and validation for simultaneous estimation of Lansoprazole and Aspirin by UV-Spectrophotometric method in pharmaceutical dosage form. In UV method Methanol as solvent and λmax of Lansoprazole and Aspirin were found to be 232nm and 222nm respectively. Concentration ranges were found to be 4-20μg/mL for both drugs. The R² values were found to be 0.996 and 0.999 for Lansoprazole and Aspirin respectively. The method was validated statistically and by recovery studies. Percentage Assay and Recovery were found to be 95-105% for Lansoprazole and Aspirin. LOD and LOQ ranges were found to be 0.177 and 0.539μg/mL and 0.298 and 0.903μg/mL for Lansoprazole and Aspirin repectively. This method was validated using ICH guidelines.

Keywords

Aspirin, Lansoprazole, UV-Spectrophotometric Method, Simultaneous Equation, Validation.

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G. Kumaraswamy ¹, M. A. Zeeshan Hamza ², R. Suthakaran ²

Affiliations
1 Jawaharlal Nehru Technological University Kakinada, Kakinada -533003.A.P., IN
2 Department of Pharmaceutical analysis & QA, Teegala Ramreddy College of Pharmacy, Meerpet, Hyderabad, 500097, Telangana, IN

Abstract

The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium tablets .The chromatographic separation was achieved on a Inertsil C18 ODS(4.6 x 250mm, 5􀀀m) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins. The retention time of Dicloxacillin Sodium and Cefpodoxime Proxetil were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.

Keywords

RP-HPLC, Cefpodoxime Proxetil, Dicloxacillin Sodium, Tablet Dosage Forms, RP-HPLC Method.

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M. A. Zeeshan Hamza ¹, G. Kumaraswamy ², Gandla Lalitha ³, R. Suthakaran ¹

Affiliations
1 Department of Pharmaceutical Analysis and QA, Teegala Ramreddy College of Pharmacy, Meerpet, Hyderabad-500097, Telangana, IN
2 Pharmaceutical Analysis, Jawaharlal Nehru Technological University Kakinada, Kakinada- 533003. A.P., IN
3 Chilkur Balaji College of Pharmacy, Aziz Nagar, Hyderabad, Telangana, IN

Abstract

The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium tablets. The chromatographic separation was achieved on a Inertsil C18 ODS (4.6 x 250mm, 5μm) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10 mins.The retention time of Dicloxacillin Sodium and Cefpodoxime Proxetil were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.

Keywords

RP-HPLC, Cefpodoxime Proxetil, Dicloxacillin Sodium, Tablet Dosage Forms, RP-HPLC Method.

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G. Kumaraswamy ¹, Gandla Lalitha ², K. Swetha ³, R. Suthakaran ³, G. Ramesh Babu ⁴

Affiliations
1 Department of Pharmaceutical Analysis, Jawaharlal Nehru Technological University Kakinada, Kakinada-533003, Andhra Pradesh, IN
2 Chilkur Balaji College of Pharmacy, Aziz Nagar, Hyderabad, Telangana, IN
3 Department of Pharmaceutical Analysis and QA, Teegala Ram Reddy College of Pharmacy, Meerpet, Hyderabad-500097, Telangana, IN
4 Chaithanya College of Pharmacy, Markapur, Prakasham (Dist), A.P., IN

Abstract

The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Pseudoephedrine and Terfinadine tablets .The chromatographic separation was achieved on a Inertsil C18 ODS (4.6×250 mm, 5 μm) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins.The retention time of Pseudoephedrine and Terfinadine were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.

Keywords

RP-HPLC Method, Pseudoephedrine and Terfinadine, Tablet Dosage Forms, PDA Detection.

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K. Anandakumar ¹, G. Kumaraswamy ¹, T. Ayyappan ¹, A. S. K. Sankar ¹, D. Nagavalli ¹

Affiliations
1 Adhiparasakthi College of Pharmacy, Melmaruvathur-603319, Tamil Nadu, IN

Abstract

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Eszopiclone (ESZ) in bulk and in tablet dosage form. Isocratic elution at a flow rate of 1 ml/ min was employed on a Phenomenax Luna C₁₈ column (150×4.6 mm; 5 μ) at ambient temperature. The mobile phase consisted of Acetonitrile: phosphate buffer adjusted to pH 2.5 (25:75% v/v). The UV detection wavelength was 304 nm and 20 μl of sample was injected. The retention time for ESZ was 3.92 min. The method obeys Beer's law in the concentration range of 4-24 μg/ml. The amount of Eszopiclone Present in the formulation was found to be 99.60±1.5389. The % recovery was in the range between 99.25% and 99.86%. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the standard analytical procedures and statistical parameters. The method was successfully applied for routine analysis of ESZ in bulk and in formulation.

Keywords

Eszopiclone, RP-HPLC, UV Detection and External Standard Calibration Method.

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