Informatics Publishing Limited

Endang Suprihati ¹, Wiwik Misaco Yuniarti ²

Affiliations
1 Department of Parasitology, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya-60115, ID
2 Department of Clinical Science, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya-60115, ID

Abstract

Aim: The objective of this research was to determine the species and strains of Leucocytozoon caulleryi and study the phylogenetics of L. caulleryi of broiler chickens in endemic areas in Indonesia.
Materials and Methods: Blood samples were collected from broiler chickens originated from endemic area in Indonesia, i.e., Pasuruan, Lamongan, Blitar, Lumajang, Boyolali, Purwokerto, and Banjarmasin in 2017. Collected blood was used for microscopic examination, sequencing using BLAST method to identify the nucleotide structure of cytochrome b (cyt b) gene that determines the species, and the phylogenetics analysis of L. caulleryi that infected broiler chickens in endemic areas in Indonesia, using Mega 5 software.
Results: The results showed that Plasmodium sp. and L. caulleryi were infected broiler chickens in endemic areas in Indonesia. L. caulleryi in one area had very close phylogenetic relations with those in other areas. The genetic distance between L. caulleryi taxa from various endemic areas is very close (<5%).
Conclusion: There is a very close phylogenetics among strains of L. caulleryi that infected broiler chickens in various endemic areas in Indonesia.

Keywords

Broiler Chickens, Cytochrome B Gene, Endemic, Indonesia, Leucocytozoon caulleryi.

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Muhammad Rama Imam Saputra ¹, April H. Wardhana ², Mufasirin ³, Setiawan Koesdarto ³, Lucia Tri Suwanti ³, Nunuk Dyah Retno Lastuti ³, Endang Suprihati ³

Affiliations
1 Disease and Veteriner Public Health Science, Airlangga University, ID
2 Department of Parasitology, Veterinery Center of Bogor, ID
3 Department of Parasitology, Airlangga University, ID

Abstract

Trypanosomiasis or surra is a disease caused by blood parasite of Trypanosoma evansi. Trypanosomiasis disease in Indonesia includes Strategic Diseases of Infectious Diseases (SDID). The purpose of this study is to detect Trypanosomiasis in horses in Bima, Indonesia. A total of 90 horse blood samples were used for the study. Sample examination using blood smears technique with Giemsa staining and Card Agglutination Test of Trypanosoma evansi (CATT). The result of examination of blood samples by microscope obtained 3 positive samples infected T. evansi and by CATT obtained 1 positive samples containing antibodies against T. evansi. It was concluded that trypanosomiasis in horses in Bima was 3.3%.

Keywords

Bima, Blood Smears, Card Agglutination Test Of Trypanosoma Evansi (CATT), Horse, Trypanosoma Evansi.

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