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Sahni, Divya
- Isolation and Identification of Vibrio Species by Multiplex PCR and Investigation of its Prevention by Using Indian Spices
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Authors
Affiliations
1 Department of Microbiology, Sai Nath University, Ranchi (Jharkhand), IN
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), IN
3 Indian Council of Agricultural Research, Central Soil Salinity Research Institute Regional Research Station, Lucknow (U.P.), IN
1 Department of Microbiology, Sai Nath University, Ranchi (Jharkhand), IN
2 Division of Biotechnology, Cyto Gene Research and Development, Lucknow (U.P.), IN
3 Indian Council of Agricultural Research, Central Soil Salinity Research Institute Regional Research Station, Lucknow (U.P.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 307-314Abstract
The present study focuses the incidence of Vibrio spp. in water bodies which are used by people in surroundings for their daily needs. Vibrio constitute of both pathogenic and non-pathogenic species, thus, for differentiating between them a rapid method named multiplex PCR assay has been for the study. The method used was to first determine the physical and chemical constituents of all the 15 water samples (pond water) collected from different regions of Uttar Pradesh. Isolation of bacteria was done on selective media (TCBS) which showed both green and yellow colonies. These colonies were cultured separately on non selective media. All the pure cultures obtained were thus, used to extract their DNA using Phenol chloroform method. DNA was then visualized on agarose gel and quantified using double beam spectrophotometer. Samples with higher yield of DNA were used for amplification using species specific primers by multiplex PCR. The results were documented and thus, study derived the information that most of the waterbodies showed presence of pathogenic Vibrio species while few of them showed presence of non-pathogenic Vibrio spp.Keywords
mPCR, Vibrio, Chemical Parameters, Water, Amplification, Species Specific.
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- Pesticides use and its Effect on Soil Bacteria and Fungal Populations, Microbial Biomass Carbon and Enzymatic Activity
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Authors
Affiliations
1 ICAR-Central Soil Salinity Research Institute, Regional Research Station, Lucknow 226 002, IN
2 ICAR-National Centre for integrated Pest Management, New Delhi 110 012, IN
3 Krishi Vigyan Kendra, Sitapur II, Katia, Sitapur 261 145, IN
1 ICAR-Central Soil Salinity Research Institute, Regional Research Station, Lucknow 226 002, IN
2 ICAR-National Centre for integrated Pest Management, New Delhi 110 012, IN
3 Krishi Vigyan Kendra, Sitapur II, Katia, Sitapur 261 145, IN
Source
Current Science, Vol 116, No 4 (2019), Pagination: 643-649Abstract
The indiscriminate use of chemical pesticides disturbs the soil environment, thereby affecting flora and fauna including soil microflora, and also the physicochemical properties of the soil, viz. pH, salinity, alkalinity, leading to poor soil fertility. With the application of pesticides, possibilities exist that these chemicals may exert certain effects on non-target soil microorganisms. The soil microbial biomass plays an important role in the soil ecosystem, where they fulfill a crucial role in nutrient cycling and decomposition. A series of field experiments were conducted at farmer’s field at Sitapur, UP with different modules of integrated pest management (IPM) including use of safe formulations. The impact of application of these pesticides on the soil properties including soil microbial populations, microbial biomass carbon and enzymatic activity was assessed. It was observed that soil microbial biomass C content and dehydrogenase activity was influenced by IPM interventions.Keywords
Dehydrogenase, Fungal Population, Microbial Biomass C, Pesticides, Soil Bacteria.Full Text
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