Informatics Publishing Limited

V. Rajamanickam ¹, Dharmaraj Santhosam ¹, D. Sridharan ¹, A. Thenmozhi ¹

Affiliations
1 Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil-626190, Tamil Nadu, IN

Abstract

A simple, reverse phase high performance liquid chromatographic (HPLC) method was developed for the determination of Risperidone and Trihexyphenidyl hydrochloride in combined tablet dosage form. A octadecyl silane (ODS) C₁₈ Phenomenex Gemini (250 mm X 4.6 mm, i.d., 5μ) column , with mobile phase of 0.3% ortho phosphoric acid buffer, acetonitrile and methanol (50:30:20% v/v/v) adjusted to pH3 with triethylamine was used. The flow rate was 1 mL/minute and the effluent was monitored at 220 nm. The retention times for Risperidone and Trihexyphenidyl hydrochloride were 2.685±0.012 and 5.912±0.030 minutes respectively. The linearity range was found to be 4.8 μg/mL to 19.2 μg/mL for Risperidone and 3.2 μg/mL to 12.8 μg/mL for Trihexyphenidyl hydrochloride. The proposed method was validated as per ICH guidelines.

Keywords

Risperidone, Trihexyphenidyl Hydrochloride, RP-HPLC, Tablets.

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