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Kandaswamy, Deivanayagam
- Identification TIMP-1 and TIMP-2 in Human Radicular Dentine - In Vitro Study
Abstract Views :436 |
PDF Views:117
Authors
Kandaswamy Eswar
1,
Rubin Mohamed Ismail
1,
Hannah Rosaline
1,
Nagendrababu Venkateshbabu
1,
Deivanayagam Kandaswamy
1
Affiliations
1 Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, IN
1 Department of Conservative Dentistry and Endodontics, Sri Ramachandra University, IN
Source
Journal of Academy of Dental Education, Vol 1, No 2 (2014), Pagination: 12-16Abstract
Aim: To identify TIMP – 1 and TIMP – 2 in human radicular dentine using confocal laser scanning microscopy. Materials and Methods: Thirty freshly extracted non carious human single ischolar_mained pre molars were obtained and stored in isotonic saline at -70°C prior to use. All the teeth were decoronated at the CEJ using a diamond. Teeth were divided into 2 groups (Group 1: TIMP-1 analysis n = 15; Group 2: TIMP-2 analysis n = 15). Teeth were sectioned using a hard tissue microtome, mounted and viewed under confocal laser scanning microscopy. Results: TIMP-1 and TIMP-2 were detected in radicular dentine and were seen to be distributed more towards the inner dentine layer closer to the pulp. Conclusion: Due to a shorter half life of TIMP-1 and 2 as compared to the MMP, there is a need to use MMP inhibitors prior to obturation of the ischolar_main canal.Keywords
Bond Degradation, Collagenolysis, Confocal Laser Scanning Microscopy, Matrix Metalloproteinase, Radicular Dentine, Tissue Inhibitors of Metalloproteinase.Full Text
References
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- An Invitro Comparison of Dissolution of Human Pulp in Sodium Hypochlorite, Ethanolic Extract and Digestive Secretion of Insectivorous Plants
Abstract Views :486 |
PDF Views:0
Authors
Ramachandran Tamilselvi
1,
Hannah Rosaline
2,
Ilango Porkodi
3,
Arunakumari Veronica
4,
Deivanayagam Kandaswamy
2
Affiliations
1 Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Narayanapuram, Pallikaranai, Chennai, Tamil Nadu, IN
2 Department of Conservative Dentistry & Endodontics, Sri Ramachandra University, Chennai, IN
3 Department of Conservative Dentistry & Endodontics, Best Dental College, Madurai, IN
4 Department of Conservative Dentistry & Endodontics, Madha Dental College, Chennai, IN
1 Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Narayanapuram, Pallikaranai, Chennai, Tamil Nadu, IN
2 Department of Conservative Dentistry & Endodontics, Sri Ramachandra University, Chennai, IN
3 Department of Conservative Dentistry & Endodontics, Best Dental College, Madurai, IN
4 Department of Conservative Dentistry & Endodontics, Madha Dental College, Chennai, IN
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 942-946Abstract
The tissue dissolution property of irrigating solutions is important for successful endodontic treatment. Sodium hypochlorite (NaOCl) is a well-known tissue dissolvent, but no data have been published on herbal alternative to NaOCl. The purpose of this study was to investigate the pulp tissue dissolution capacity of sodium hypochlorite, ethanolic extract of Drosera rotundifolia and digestive fluid of Nepenthes khasiana. Freshly extracted intact vital teeth, extracted for orthodontic and impaction reasons were collected and stored at – 20 ˚C. The pulp tissues were removed by splitting of the teeth when required.
Method: The pulp tissues were randomly divided into 4 groups (n = 10), individual sample was weighed 7 mg each. The groups were as follows, Group I: 5% NaOCl, Group II: digestive fluid of Nepenthes khasiana, Group III: Drosera rotundifolia (ethanolic extract), Group IV: distilled water. The test solutions were added to tubes containing tissue samples, the tissues were removed from the test solutions at 5, 15, 20, 30 min, 1,2,3,4,5,6,7 hr blotted dry with the tissue paper and weighed using precision balance.
Results: The results showed complete dissolution of pulp tissue in Group I at 20 minutes, Group II at 7 hr, Group III and IV showed no dissolution of pulp at the investigated time intervals.
Conclusion: The digestive fluid of Nepenthes khasiana could be considered as an herbal alternative to NaOCl for pulp tissue dissolution as it completely dissolved the pulp tissue similar to 5% NaOCl, but at different time intervals.
