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Semwal, Deepak Kumar
- Speckle-Type POZ Protein Acts as a Predictive Biomarker for MTOR Target Therapy in Renal Cell Carcinoma
Abstract Views :402 |
PDF Views:152
Authors
Ashutosh Chauhan
1,
Rani Ojha
1,
Deepak Kumar Semwal
2,
Satyendra Prasad Mishra
3,
Ruchi Badoni Semwal
4
Affiliations
1 Department of Urology, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, IN
2 Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
3 Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
4 Department of Pharmaceutical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, ZA
1 Department of Urology, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, IN
2 Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
3 Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
4 Department of Pharmaceutical Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, ZA
Source
Current Science, Vol 111, No 12 (2016), Pagination: 2014-2019Abstract
Renal cell carcinoma (RCC) develops resistance to most of the conventional therapies. In recent years inhibitors are being used to interfere with growth of cancer cells at molecular level and are found to be the most effective treatment option. However, some patients have to deal with severe side effects. Till date there have been no specific predictive biomarkers available to predict the target response and patient's stratification. We evaluate the expression of speckletype POZ protein (SPOP) as a predictive biomarker in the presence of mTOR target therapy. Tissue samples were collected after nephrectomies from patients with RCC. Further primary culture was established from tissues of low- and high-grade RCC. Working concentration of inhibitors (CCI-779 and SB203580) was selected by MTT assay on A-498 cell line. Western blot was performed to study the p38MAPK, mTOR and SPOP protein expression. Primary culture showed more than 70% positivity for pan-cytokeratin. The concentration of 20 μM of CCI-799 and 25 μM SB203580 caused 30% and 20% cell death respectively. Expression of SPOP protein was decreased in CCI-779-treated cells. The combined treatment of CCI-779 and SB203580 had more inhibitory effects on SPOP in all cells types. However, SB203580 had no effect on SPOP expression. The results underline that, SPOP could be used as a potential predictive biomarker to assess the response of therapy with mTOR inhibitor (CCI-779).Keywords
Protein Expression, Predictive Biomarker, Renal Cell Carcinoma, Target Therapy.- In vitro Micropropagation, Total Phenolic Content and Comparative Antioxidant Activity of Different Extracts of Sesbania grandiflora (L.) Pers.
Abstract Views :499 |
PDF Views:176
Authors
Krishnamoorthy Vinothini
1,
Masilamani Sri Devi
1,
Veronica Shalini
1,
Sudharshan Sekar
2,
Ruchi Badoni Semwal
3,
Pandian Arjun
3,
Deepak Kumar Semwal
4
Affiliations
1 Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, IN
2 Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park-2006, ZA
3 Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, ZA
4 Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
1 Department of Biotechnology, Jeppiaar Engineering College, Old Mamallapuram Road, Rajiv Gandhi Salai, Chennai 600 119, IN
2 Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park-2006, ZA
3 Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria 0001, ZA
4 Department of Phytochemistry, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
Source
Current Science, Vol 113, No 06 (2017), Pagination: 1142-1147Abstract
Sesbania grandiflora (L.) Pers. is a common traditional medicinal plant used in bronchitis, anaemia,headache, fever, ophthalmia, nasal catarrh, leprosy, inflammation, gout and rheumatism. The present study aimed to assess plant regeneration and plantlets development in vitro using explants of S. grandiflora together with the estimation of total phenolic contentand antioxidative activity of various extracts obtained from the plant. Murashige and Skoog (MS) basal medium added with different concentrations of plant growth regulators (PGRs) was used for plant tissue culture, whereas ferric reducing antioxidant power(FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)assays were used to evaluate the antioxidant potential of different extracts of the plant. In the presence of 6-benzylaminopurine (BAP; 0.1 mg/l), the highest level(85.41%) of seed germination was achieved while the highest callus formation (96.6%) was recorded with 2,4-dichlorophenoxyacetic acid (2,4-D; 0.5 mg/l). Inaddition, the highest shoot induction, shoot formation and shoot elongation were observed with BAP(0.1 mg/l), indole-3-butyric acid (3 mg/l) and naphthaleacetic acid + BAP (0.4 + 0.2 mg/l) respectively.The extract of dried calluses showed highest contents of proline (110.94 mg/g), phenol (16.42 mg/g) and flavonoid (22.22 mg/g), and also highest antioxidant potential with FRAP and DPPH assays. From the present study, we may conclude that the MS basal medium supplemented with PGRs is effective for the commercial production of S. grandiflora.Keywords
Antioxidant Activity, In vitro Micropropagation, Phenolic Content, Plant Growth Regulators, Sesbania grandiflora.References
- Shyamala Gowri, S. and Vasantha, K., Free radical scavenging and antioxidant activity of leaves from Agathi (Sesbania grandiflora L.) Pers. Am.-Eurasian J. Sci. Res., 2010, 5, 114–119.
- Anon., The Wealth of India: A Dictionary of Raw Materials and Industrial Products, CSIR (Govt of India), New Delhi, 1972, pp. 275–299.
- Shareef, H., Rizwani, G. H., Zia-ul-Haq, M., Ahmad, S. and Zahid, H., Tocopherol and phytosterol profile of Sesbania grandiflora (Linn.) seed oil. J. Med. Plants Res., 2012, 6, 3478–3481.
- Sheakh, A. A., Sayyed, Z., Siddiqui, A. R., Pratapwar, A. S. and Sheakh, S. S., Wound healing activity of Sesbania grandiflora Linn. flower ethanolic extract using excision and incision wound model in wistar rats. Int. J. PharmTech Res., 2011, 3, 895–898.
- Arjun, P., Investigations on microprogation and isolation of active compounds from Tribulus terrestris Linn. Ph D thesis, Faculty of Sciences, University of Madras, Chennai, 2011.
- Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant., 1962, 15, 473–497.
- Malaisamy, M. and Mohan, N., Impact of ozone on morphological, physiological, and biochemical changes in cow pea (Vigna unguiculata [L.] Walp.). Ozone: Sci. Eng., 2014, 36, 36–42.
- Arjun, P., Samwal, D. K., Samwal, R. B., Anita Blessy Vijayan and Krishnamoorthy, M., Quality retention and shelf-life improvement of fresh-cut apple, papaya, carrot and cucumber by chitosan-soy based edible coating. Curr. Nutr. Food Sci., 2015, 11, 282–291.
- Kim, D. O., Jeong, S. W. and Lee, C. Y., Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem., 2003, 81, 321–326.
- Xu, B. J. and Chang, S. K. C., A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J. Food Sci., 2007, 72, 159–166.
- Nagarajan, S., Arjun, P., Raaman, N., Anup, S., Elizabeth Sobhia, M. and Mohan Das, T., Selective synthesis of sugar-based β-lactum derivatives: docking studies and its biological evaluation. Tetrahydron, 2012, 68, 3037–3045.
- Asghari, G. and Lockwood, B. G., Stereospecific biotransformation of phenylethyl propionate by cell cultures of Peganum harmala L. Iran. Biomed. J., 2002, 6, 43–46.
- Mohan, J., Kumar, V. V., Aparna, V. and Vaidya, R. P., Somatic embryogenesis and plant regeneration in Tribulus terrestris L. Phytomorphology, 2000, 50, 307–311.
- Gurel, E. and Gurel, S., Plant regeneration from unfertilized ovaries of sugar beet (Beta vulgaris L.) cultured in vitro. Traditional J. Bot., 1998, 22, 233–238.
- Sharma, P. and Rajam, Genotype, explant and position effects on organogenesis and somatic embryogenesis in egg plant (Solanum melongena L.). J. Exp. Bot., 1995, 46, 135–141.
- Pande, D., Malik, S., Bora, M. and Srivastava, P. S., Rapid protocol for in vitro micropropagation of Lepidium sativum Linn. and enhancement in the yield of lepidine. In Vitro Cell. Dev. Biol., 2002, 38, 451–455.
- Szabados, L. and Savoure, A., Proline: a multifunctional amino acid. Trends Plant Sci., 2009, 15, 89–97.
- Uttara, B., Singh, A. V., Zamboni, P. and Mahajan, R., Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr. Neuropharmacol., 2009, 7, 65–74.
- Harborne, J. B. and Williams, C. A., Advances in flavonoid research since 1992. Phytochemistry, 2000, 55, 481–504.
- Benzie, I. F. and Strain, J. J., The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power the FRAP assay. Anal. Biochem., 1996, 239, 70–76.
- Arjun, P., Saranya Sivan, P. S., Mohana Priya, S., Krishnamoorthy, M. and Balasubramanian, K., Antioxidant and antimicrobial activity of Nelumbo nucifera Gaertn. leaf extracts. J. Acad. Indust. Res., 2012, 1, 15–18.
- Muthu Kumar, T., Mary Violet Christy, A., Mangadu, A., Malaisamy, M., Sivaraj, C., Arjun, P. and Raaman, N., Antioxidant and anticancer activity of Helicteres isora dried fruit solvent extracts. J. Acad. Indust. Res., 2012, 1, 148–152.
- Hariprasath, L., Jegadeesh, R., Arjun, P. and Raaman, N., In vitro propagation of Senecio candicans DC and comparative antioxidant properties of aqueous extracts of the in vivo plant and in vitro derived callus. S. Afr. J. Bot., 2015, 98, 134–141.
- Balasundram, N., Sundram, K. and Samman, S., Phenolic compounds in plant and agri-industrial byproducts: antioxidant activity, occurrence, and potential uses. Food Chem., 2006, 99, 191–203.
- Duh, P. D., Tu, Y. Y. and Yen, G. C., Antioxidant activity of aqueous extract of Hamjyur (Chrsanthemum morifolium Ramat). Lebensm. Wiss. Technol., 1999, 32, 269–277.
- Natural Molecules Having Anti-SARS-CoV Activity – Cannot They Be Effective Against SARS-CoV-2?
Abstract Views :413 |
PDF Views:144
Authors
Deepak Kumar Semwal
1,
Ashutosh Chauhan
2,
Ruchi Badoni Semwal
3,
Debabrata Sircar
4,
Partha Roy
4,
Jörg Lehmann
5
Affiliations
1 Department of Phytochemistry, Faculty of Biomedical Sciences, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
2 Department of Biotechnology, Faculty of Biomedical Sciences, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
3 Department of Chemistry, Pt Lalit Mohan Sharma Government Postgraduate College, Rishikesh 249 201, IN
4 Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, IN
5 Department of Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Perlickstr. 1, 04103 Leipzig, DE
1 Department of Phytochemistry, Faculty of Biomedical Sciences, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
2 Department of Biotechnology, Faculty of Biomedical Sciences, Uttarakhand Ayurved University, Harrawala, Dehradun 248 001, IN
3 Department of Chemistry, Pt Lalit Mohan Sharma Government Postgraduate College, Rishikesh 249 201, IN
4 Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, IN
5 Department of Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Perlickstr. 1, 04103 Leipzig, DE