Informatics Publishing Limited

Basavaraj R. Tubaki ¹, Anupriya Verma ¹, Arun Sasidharan ¹, S. Sulekha ¹, T. N. Sathyaprabha ¹, D. Sudhakar ², C. R. Chandrashekar ³, G. S. Lavekar ⁴, Bindu M. Kutty ¹

Affiliations
1 Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Bengaluru 560 029, IN
2 Advanced Centre for Ayurveda, National Institute of Mental Health and Neurosciences, Bengaluru 560 029, IN
3 Department of Psychiatry, National Institute of Mental Health and Neurosciences, Bengaluru 560 029, IN
4 Central Council for Research in Ayurvedic Sciences, New Delhi 110 058, IN

Abstract

This study demonstrates the clinical efficacy of Manasamitra Vataka and Shirodhara (Ayurvedic treatments) over clonazepam in preserving slow wave sleep and promoting sleep quality in patients of generalized anxiety disorder (GAD) with co-morbid generalized social phobia. Whole night polysomnography was carried out to assess the sleep architecture and spindle- delta dynamics. The study highlights the sleep promoting and preserving nature of Manasamitra Vataka and Shirodhara in GAD patients with co-morbid generalized social phobia. Ayurvedic treatments were helpful in improving the subjective quality of sleep and preserve sleep organization. Further studies are needed to confirm the potential of Ayurvedic interventions as a treatment of choice in the management of anxiety disorders.

Keywords

Clonazepam, Generalized Anxiety Disorder, Manasamitra vataka, Shirodhara, Sleep Architecture.

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R. Manikandan ¹, P. Kanimozhi ¹, N. Balakrishnan ¹, V. Balasubramani ¹, D. Sudhakar ¹, V. Udayasuriyan ¹

Affiliations
1 Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

A novel synthetic cry2AX1 gene was codon optimized and a sequence encoding cotton rbcS1b transit peptide was fused upstream of coding sequence. The fusion cry2AX1 gene, driven by maize ubiquitin1 promoter was cloned in a pUH plant transformation vector. Agrobacterium mediated transformation was carried out with pUH-ctp-2AX1 construct using leaf discs of tobacco as model plant. Screening by PCR revealed presence of cry2AX1 gene in all nine putative transformants and expression of cry2AX1 protein in PCR positive T₀ tobacco plants ranged from 1.5 to 10.0 ng/g. The detached leaf bit bioassay of tobacco transformants with Helicoverpa armigera showed 30 per cent mortality even at lower level of cry2AX1 expression. The results indicated a newly developed construct was functionally expressed in tobacco plant.

Keywords

Tobacco, Transformation, Insect Resistance, Helicoverpa armigera.

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